全文获取类型
收费全文 | 257266篇 |
免费 | 16086篇 |
国内免费 | 155篇 |
出版年
2018年 | 2701篇 |
2017年 | 2813篇 |
2016年 | 4982篇 |
2015年 | 8086篇 |
2014年 | 8505篇 |
2013年 | 9500篇 |
2012年 | 9307篇 |
2011年 | 7162篇 |
2010年 | 5690篇 |
2009年 | 5268篇 |
2008年 | 5333篇 |
2007年 | 5550篇 |
2006年 | 5280篇 |
2005年 | 10993篇 |
2004年 | 9874篇 |
2003年 | 8009篇 |
2002年 | 5630篇 |
2001年 | 9310篇 |
2000年 | 8879篇 |
1999年 | 7571篇 |
1998年 | 2281篇 |
1997年 | 2187篇 |
1996年 | 1890篇 |
1992年 | 6827篇 |
1991年 | 7009篇 |
1990年 | 6861篇 |
1989年 | 6753篇 |
1988年 | 6340篇 |
1987年 | 5976篇 |
1986年 | 5445篇 |
1985年 | 5565篇 |
1984年 | 4320篇 |
1983年 | 3593篇 |
1982年 | 2332篇 |
1981年 | 2083篇 |
1980年 | 2038篇 |
1979年 | 4103篇 |
1978年 | 3179篇 |
1977年 | 2780篇 |
1976年 | 2603篇 |
1975年 | 3245篇 |
1974年 | 3686篇 |
1973年 | 3630篇 |
1972年 | 3431篇 |
1971年 | 3155篇 |
1970年 | 2792篇 |
1969年 | 2719篇 |
1968年 | 2511篇 |
1967年 | 2339篇 |
1966年 | 1931篇 |
排序方式: 共有10000条查询结果,搜索用时 31 毫秒
961.
962.
I M Badalova B N Mishan'kin I Iu Suchkov 《Zhurnal mikrobiologii, epidemiologii, i immunobiologii》1987,(5):24-28
In two different strains of cholera vibrios two recA-dependent plasmids, pVib I (1.9-2.2 Md) and pVib II (5.2-5.8 Md), have been detected. These plasmids determine the synthesis of vibriocin, coagulase and fibrinolysin, which has been established by the cotransformation of the DNA of plasmids pVib I and pBR322 and by the transfer mobilization with the use of plasmid RP4. 相似文献
963.
964.
965.
966.
The steady-state kinetics of Pseudomonas aeruginosa cytochrome oxidase were studied. Reduced cytochrome c551 and azurin from the same bacteria were used as the electron-donating substrates, while dioxygen served as the electron acceptor. Oxidized cytochrome c551 and azurin exhibited product inhibition of the reaction. However, apo-azurin and azurin derivatives in which the copper was substituted by the redox-inert ions Ni2+, Co2+, Cd2+ and Zn2+, did not show any effect on the kinetics. These observations implied that complex formation between the substrates or the products and the enzyme is not a rate-limiting step and is not the cause for product inhibition. The integrated rate law for a reaction scheme in which we assumed that complex formation was not rate limiting was fitted to the complete reaction traces. The results suggested that it is the low thermodynamic driving force, expressed in the small differences in redox potential between the substrates and heme c of the enzyme, which cause the observed product inhibition. 相似文献
967.
968.
Christian Renz Silke Oeljeklaus S?ren Grinhagens Bettina Warscheid Nils Johnsson Thomas Gronemeyer 《PloS one》2016,11(2)
The septins are a conserved family of GTP-binding proteins that, in the baker''s yeast, assemble into a highly ordered array of filaments at the mother bud neck. These filaments undergo significant structural rearrangements during the cell cycle. We aimed at identifying key components that are involved in or regulate the transitions of the septins. By combining cell synchronization and quantitative affinity-purification mass-spectrometry, we performed a screen for specific interaction partners of the septins at three distinct stages of the cell cycle. A total of 83 interaction partners of the septins were assigned. Surprisingly, we detected DNA-interacting/nuclear proteins and proteins involved in ribosome biogenesis and protein synthesis predominantly present in alpha-factor arrested that do not display an assembled septin structure. Furthermore, two distinct sets of regulatory proteins that are specific for cells at S-phase with a stable septin collar or at mitosis with split septin rings were identified.Complementary methods like SPLIFF and immunoprecipitation allowed us to more exactly define the spatial and temporal characteristics of selected hits of the AP-MS screen. 相似文献
969.
970.