全文获取类型
收费全文 | 672613篇 |
免费 | 76922篇 |
国内免费 | 226篇 |
专业分类
749761篇 |
出版年
2016年 | 7354篇 |
2015年 | 9506篇 |
2014年 | 11716篇 |
2013年 | 16665篇 |
2012年 | 18581篇 |
2011年 | 19185篇 |
2010年 | 12864篇 |
2009年 | 12036篇 |
2008年 | 17190篇 |
2007年 | 18172篇 |
2006年 | 16906篇 |
2005年 | 16323篇 |
2004年 | 16393篇 |
2003年 | 15862篇 |
2002年 | 15386篇 |
2001年 | 29004篇 |
2000年 | 29629篇 |
1999年 | 23270篇 |
1998年 | 8192篇 |
1997年 | 8616篇 |
1996年 | 8198篇 |
1995年 | 7817篇 |
1994年 | 7789篇 |
1993年 | 7745篇 |
1992年 | 20243篇 |
1991年 | 20295篇 |
1990年 | 19825篇 |
1989年 | 19426篇 |
1988年 | 18103篇 |
1987年 | 17307篇 |
1986年 | 16154篇 |
1985年 | 16439篇 |
1984年 | 13694篇 |
1983年 | 12028篇 |
1982年 | 8988篇 |
1981年 | 8347篇 |
1980年 | 7921篇 |
1979年 | 13515篇 |
1978年 | 10579篇 |
1977年 | 9690篇 |
1976年 | 9153篇 |
1975年 | 10371篇 |
1974年 | 10988篇 |
1973年 | 10753篇 |
1972年 | 10178篇 |
1971年 | 8967篇 |
1970年 | 7894篇 |
1969年 | 7428篇 |
1968年 | 6813篇 |
1967年 | 6022篇 |
排序方式: 共有10000条查询结果,搜索用时 0 毫秒
61.
62.
63.
Trigger finger is a relatively common clinical entity, most frequently caused by stenosing tenosynovitis. Several other conditions not related to tenosynovitis also have been described as a cause of triggering, and these have been reviewed. We present a rare anomaly of the fourth lumbrical muscle insertion as a cause of triggering of the right little finger. This was completely relieved following excision of the anomalous muscle. This rare anatomic variant should be added to the list of potential causes of trigger finger. 相似文献
64.
65.
66.
Metabolic pathways involved in the oxidation of isopropanol into acetone by the intact rat 总被引:3,自引:0,他引:3
Isopropanol administered in a large (6 g/kg, orally) as well as in a lower dose (1 g/kg, I.P.) is slowly oxidized into acetone by the intact rat. Using two inhibitors, 3 amino-1,2,4-triazole and pyrazole, investigations on the hepatic enzymatic system involved in the oxidation of isopropanol show that catalase does not play an important part in this pathway, contrary to alcohol dehydrogenase which is the major enzyme responsible for this oxidation. Although isopropanol oxidation is mainly catalysed in the liver through alcohol dehydrogenase, no alteration of the hepatic extramitochondrial redox state occurs after the administration of a large as well as of a lower dose of isopropanol. From these experiments it may be concluded that alterations of the liver NAD+/NADH ratio, which seem to play an important part in the ethanol induced fatty liver, are not involved in the isopropanol induced one. 相似文献
67.
68.
We have previously shown that replacing the P1-site residue (Ala) of chicken ovomucoid domain 3 (OMCHI3) with a Met or Lys results in the acquisition of inhibitory activity toward chymotrypsin or trypsin, respectively. However, the inhibitory activities thus induced are not strong. In the present study, we introduced additional amino acid replacements around the reactive site to try to make the P1-site mutants more effective inhibitors of chymotrypsin or trypsin. The amino acid replacement Asp-->Tyr at the P2' site of OMCHI3(P1Met) resulted in conversion to a 35000-fold more effective inhibitor of chymotrypsin with an inhibitor constant (K(i)) of 1. 17x10(-11) M. The K(i) value of OMCHI3(P1Met, P2'Ala) indicated that the effect on the interaction with chymotrypsin of removing a negative charge from the P2' site was greater than that of introducing an aromatic ring. Similarly, enhanced inhibition of trypsin was observed when the Asp-->Tyr replacement was introduced into the P2' site of OMCHI3(P1Lys). Two additional replacements, Asp-->Ala at the P4 site and Arg-->Ala at the P3' site, made the mutant a more effective inhibitor of trypsin with a K(i) value of 1. 44x10(-9) M. By contrast, Arg-->Ala replacement at the P3' site of OMCHI3(P1Met, P2'Tyr) resulted in a greatly reduced inhibition of chymotrypsin, and Asp-->Ala replacement at the P4 site produced only a small change when compared with a natural variant of OMCHI3. These results clearly indicate that not only the P1-site residue but also the characteristics, particularly the electrostatic properties, of the amino acid residues around the reactive site of the protease inhibitor determine the strength of its interactions with proteases. Furthermore, amino acids with different characteristics are required around the reactive site for strong inhibition of chymotrypsin and trypsin. 相似文献
69.
Z Zhong A Toukdarian D Helinski V Knauf S Sykes J E Wilkinson C O'Bryne T Shea C DeLoughery R Caspi 《Applied and environmental microbiology》2001,67(12):5771-5779
An agar-degrading marine bacterium identified as a Microscilla species was isolated from coastal California marine sediment. This organism harbored a single 101-kb circular DNA plasmid designated pSD15. The complete nucleotide sequence of pSD15 was obtained, and sequence analysis indicated a number of genes putatively encoding a variety of enzymes involved in polysaccharide utilization. The most striking feature was the occurrence of five putative agarase genes. Loss of the plasmid, which occurred at a surprisingly high frequency, was associated with loss of agarase activity, supporting the sequence analysis results. 相似文献
70.
J R Silkensen A P Skubitz K M Skubitz M E Rosenberg 《The journal of peptide research》1999,54(5):449-457
Expression of the glycoprotein clusterin is markedly increased following tissue injury. One function of clusterin is to promote cell interactions which are perturbed in these pathologic settings. Clusterin causes cell aggregation and adhesion in vitro yet the molecular mechanism for this effect is not known. In order to identify the active site(s) of clusterin, 34 peptides, each 15 amino acid residues in length, were synthesized from hydrophilic regions of human clusterin. When studied individually, none of the peptides caused aggregation of LLC-PK1 cells, a porcine renal epithelial cell line. However, two out of the 34 peptides inhibited clusterin-induced cell aggregation in a dose-dependent manner. Scrambled versions of these two 'active' peptides did not inhibit cell aggregation. Seven peptides promoted cell adhesion. In conclusion, these findings provide evidence for novel amino acid sequences mediating clusterin-induced renal cell interactions. 相似文献