全文获取类型
收费全文 | 589501篇 |
免费 | 62735篇 |
国内免费 | 785篇 |
专业分类
653021篇 |
出版年
2018年 | 5562篇 |
2016年 | 7474篇 |
2015年 | 9927篇 |
2014年 | 11922篇 |
2013年 | 16266篇 |
2012年 | 19092篇 |
2011年 | 19938篇 |
2010年 | 13352篇 |
2009年 | 12141篇 |
2008年 | 17595篇 |
2007年 | 18319篇 |
2006年 | 17195篇 |
2005年 | 16293篇 |
2004年 | 16479篇 |
2003年 | 15552篇 |
2002年 | 15171篇 |
2001年 | 25298篇 |
2000年 | 25448篇 |
1999年 | 19889篇 |
1998年 | 7218篇 |
1997年 | 7269篇 |
1996年 | 6813篇 |
1995年 | 6482篇 |
1994年 | 6112篇 |
1993年 | 6211篇 |
1992年 | 16556篇 |
1991年 | 16531篇 |
1990年 | 16186篇 |
1989年 | 15664篇 |
1988年 | 14603篇 |
1987年 | 13752篇 |
1986年 | 12968篇 |
1985年 | 12888篇 |
1984年 | 10605篇 |
1983年 | 9199篇 |
1982年 | 6740篇 |
1981年 | 6055篇 |
1980年 | 5743篇 |
1979年 | 9968篇 |
1978年 | 8038篇 |
1977年 | 7149篇 |
1976年 | 6675篇 |
1975年 | 7805篇 |
1974年 | 8645篇 |
1973年 | 8416篇 |
1972年 | 7672篇 |
1971年 | 7065篇 |
1970年 | 6059篇 |
1969年 | 5871篇 |
1968年 | 5435篇 |
排序方式: 共有10000条查询结果,搜索用时 0 毫秒
961.
Alexis L. Moore Joseph E. Budny Anthony P. Russell Michael T. Butcher 《Journal of morphology》2013,274(1):35-48
Evaluation of the relationships between muscle structure and digging function in fossorial species is limited. Badgers and other fossorial specialists are expected to have massive forelimb muscles with long fascicles capable of substantial shortening for high power and applying high out‐force to the substrate. To explore this hypothesis, we quantified muscle architecture in the thoracic limb of the American badger (Taxidea taxus) and estimated the force, power, and joint torque of its intrinsic musculature in relation to the use of scratch‐digging behavior. Architectural properties measured were muscle mass, belly length, fascicle length, pennation angle, and physiological cross‐sectional area. Badgers possess hypertrophied shoulder flexors/humeral retractors, elbow extensors, and digital flexors. The triceps brachii is particularly massive and has long fascicles with little pennation, muscle architecture consistent with substantial shortening capability, and high power. A unique feature of badgers is that, in addition to elbow joint extension, two biarticular heads (long and medial) of the triceps are capable of applying high torques to the shoulder joint to facilitate retraction of the forelimb throughout the power stroke. The massive and complex digital flexors show relatively greater pennation and shorter fascicle lengths than the triceps brachii, as well as compartmentalization of muscle heads to accentuate both force production and range of shortening during flexion of the carpus and digits. Muscles of most functional groups exhibit some degree of specialization for high force production and are important for stabilizing the shoulder, elbow, and carpal joints against high limb forces generated during powerful digging motions. Overall, our findings support the hypothesis and indicate that forelimb muscle architecture is consistent with specializations for scratch‐digging. Quantified muscle properties in the American badger serve as a comparator to evaluate the range of diversity in muscle structure and contractile function that exists in mammals specialized for fossorial habits. J. Morphol. 2013. © 2012 Wiley Periodicals, Inc. 相似文献
962.
Valuable biological information can be obtained by monitoring the movement of organisms. However, the choice of monitoring
method becomes highly restricted when following small organisms (<100 mm), especially in aquatic ecosystems. Stable isotopes
are being increasingly used in this respect but rarely at the local spatial scale, i.e. 10–1000 s of metres. We sought to
identify movement of small fishes between a main river channel and its tributary. Little overlap in isotope baseline was detected
between the two channels despite some temporal variability in δ15N of baseline indicator organisms in the main river. The individuals of two small cyprinid fish species (Leuciscus souffia and Alburnoides bipunctatus) of all the size classes (40–100 mm) caught within the tributary showed considerable heterogeneity in δ15N values. Classification and discriminant analysis on isotope-derived data distinguished two significantly different groups.
Moreover, this result was supported by further sampling of fish caught in the main river (in May and December 2006). Alternative
hypotheses, such as dietary differences, biological factors, temporal shifts and spatial differences in diet, did not explain
δ15N variability. This application of stable isotopes at a relatively small spatial and temporal scales further demonstrates
its potential as a tool for ecologists. 相似文献
963.
964.
965.
Jos C. García‐Borrn Berta L. Snchez‐Laorden Celia Jimnez‐Cervantes 《Pigment cell & melanoma research》2005,18(6):393-410
The melanogenic actions of the melanocortins are mediated by the melanocortin‐1 receptor (MC1R). MC1R is a member of the G‐protein‐coupled receptors (GPCR) superfamily expressed in cutaneous and hair follicle melanocytes. Activation of MC1R by adrenocorticotrophin or α‐melanocyte stimulating hormone is positively coupled to the cAMP signaling pathway and leads to a stimulation of melanogenesis and a switch from the synthesis of pheomelanins to the production of eumelanic pigments. The functional behavior of the MC1R agrees with emerging concepts in GPCR signaling including dimerization, coupling to more than one signaling pathway and a high agonist‐independent constitutive activity accounting for inverse agonism phenomena. In addition, MC1R displays unique properties such as an unusually high number of natural variants often associated with clearly visible phenotypes and the occurrence of endogenous peptide antagonists. Therefore MC1R is an ideal model to study GPCR function. Here we review our current knowledge of MC1R structure and function, with emphasis on information gathered from the analysis of natural variants. We also discuss recent data on the regulation of MC1R function by paracrine and endocrine factors and by external stimuli such as ultraviolet light. 相似文献
966.
Determining the kinetics of membrane pores from patch clamp data without measuring the open and closed times 总被引:1,自引:0,他引:1
We present a new and very time saving way to determine kinetic rate constants from patch clamp data by using the correlation functions utilized in analyzing experiments with photon correlations. 相似文献
967.
D I Medvedev I I Babichenko I Z Eremina A I Kravtsova 《Biulleten' eksperimental'no? biologii i meditsiny》1985,100(12):751-754
Electron microscopy of mouse neocortex was carried out during rehabilitation following long-term protein-caloric deficiency. Food rehabilitation led to partial recovery of brain ultrastructure, however, a high neuronal level of secondary lysosoms and lipofuscin bodies was preserved, with the changes in the spine apparatus and synaptic contacts persisting in neuropile. Carnitine addition to food during rehabilitation increased the number of free ribosomes in cortical neurons. A substantial development of granular endoplasmic reticulum was observed. A greater number of spine apparatus cisterns was detected, however, like in conventional rehabilitation, the width of synaptic clefts and postsynaptic densities remained narrower than in control mice. 相似文献
968.
K Haya B A Waiwood L Van Eeckhaute 《Comp. Biochem. Physiol. C, Comp. Pharmacol. Toxicol.》1985,82(2):323-329
Juvenile Atlantic salmon were held for 76 days at pH 4.7 during the period when the final stages of smoltification normally occur. Control salmon (pH 6.5) had significant increases in weight, length and liver somatic index which were not observed in those held at low pH. Condition factor decreased in both groups but to a significantly greater extent in the low pH group. After 15 days levels of ADP and glucose were higher and AEC, CP and glycogen were lower in muscles of salmon exposed to low pH. These qualitative differences were maintained until the end of the experiment. ATP and total adenylate concentrations in muscle were lower after 62 days of exposure to low pH compared to controls. The levels of ATP, total adenylates, AEC and glucose were consistently higher in livers of salmon exposed to low pH than those of controls. There were no differences in liver concentrations of AMP, CP and glycogen between the two treatments or with time of exposure within each treatment. The results suggest that exposure of juvenile Atlantic salmon to low pH increased gluconeogenesis and decreased food intake to the detriment of growth. 相似文献
969.
Reconstitution of RecBC DNase activity from purified Escherichia coli RecB and RecC proteins 总被引:8,自引:0,他引:8
I D Hickson C N Robson K E Atkinson L Hutton P T Emmerson 《The Journal of biological chemistry》1985,260(2):1224-1229
The Escherichia coli RecB protein, normally synthesized in low amounts, has been amplified by linkage of the recB gene to the phage lambda leftward promoter in an expression plasmid. From strains harboring this plasmid, RecB protein has been purified to homogeneity by a simple procedure which includes affinity chromatography on a column of RecC protein bound to agarose. The purified RecB protein has DNA-dependent ATPase activity but no exonuclease activity. RecC protein alone has neither ATPase nor exonuclease activity. However, when combined together, the RecB and RecC proteins show the ATP-dependent double-stranded exonuclease properties characteristic of the RecBC DNase. 相似文献
970.
Separation of Legionella pneumophila proteases and purification of a protease which produces lesions like those of Legionnaires' disease in guinea pig lung 总被引:11,自引:0,他引:11
Six discrete protease activities were recovered from the supernatant broth of Legionella pneumophila cultures by ion-exchange chromatography. One of these demonstrated in vitro activity against collagen, casein and gelatin. When administered into the lungs of guinea-pigs this protease elicited lesions which were pathologically similar to those seen in clinical and experimentally induced Legionnaires' disease. 相似文献