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91.
Colorectal cancer is the third most commonly diagnosed cancer in the world. Monoclonal antibody (mAb) CO17‐1A recognizes the tumor‐associated antigen GA733‐2, a cell surface glycoprotein highly expressed in colorectal carcinoma cells which is applicable for preventing and curing colorectal cancer. In this study, we tried to produce a new recombinant anti‐colorectal cancer large single chain (lsc) mAb based on mAb CO17‐1A in the baculovirus‐insect cell protein expression system. Two kinds of recombinant lsc mAbs were generated where variable light chain (VL) and heavy chain (HC) of mAb CO17‐1A were fused together by an interchain linker. The only difference between the two mAbs is based on fusion of an ER retention signal (KDEL) at its C‐terminus of HC. Polymerase chain reaction analysis verified the presence of both recombinant genes in the bacmid for generating viral expression vectors in insect cells. Western blot confirmed the expression of lsc mAbs in baculovirus‐infected insect cells. Cell enzyme linked immunosorbent assay (ELISA) showed that the mAbs from cell lysates bound to SW480 and SW620 human colorectal cancer cells. These results indicate that the baculovirus insect expression system can produce anti‐colorectal lsc mAb recognizing human colorectal cancer cells. 相似文献
92.
93.
Hwang JH Kim JY Cha MR Ryoo IJ Choo SJ Cho SM Tsukumo Y Tomida A Shin-Ya K Hwang YI Yoo ID Park HR 《Journal of cellular physiology》2008,215(1):243-250
Glucose deprivation, a pathophysiological cell condition, causes up-regulation of GRP78 and induction of etoposide resistance in human cancer cells. The induction of drug resistance can be partly explained by the fact that GRP78 can block activation of caspase-7 induced by treatment with etoposide. Therefore, downregulating GRP78 expression may be a novel strategy anticancer drug development. Based on that premise, we established a screening program for anticancer agents that exhibit preferential cytotoxic activity for etoposide-resistant cancer cells under glucose-deprived conditions. We recently isolated an active compound, AR-054, from the culture broth of Streptomyces sp., which prevents stress-induced etoposide resistance in vitro. AR-054 was identified as piericidin A, a prototypical compound, by ESI-MS analysis and various NMR spectroscopic methods. Here, we showed that piericidin A suppressed the accumulation of GRP78 protein and was also highly toxic to etoposide-resistant HT-29 cells, with IC50 values for colony formation of 6.4 and 7.7 nM under 2-deoxyglucose supplemented and glucose-deprived conditions, respectively. Interestingly, piericidin A had no effect under normal growth conditions. Therefore, we suggest that piericidin A prevents up-regulation of GRP78, and exhibits cytotoxicity in glucose-deprived HT-29 cells that are resistant to etoposide. 相似文献
94.
Extracellular matrix proteins protect small cell lung cancer cells against apoptosis: a mechanism for small cell lung cancer growth and drug resistance in vivo. 总被引:29,自引:0,他引:29
T Sethi R C Rintoul S M Moore A C MacKinnon D Salter C Choo E R Chilvers I Dransfield S C Donnelly R Strieter C Haslett 《Nature medicine》1999,5(6):662-668
Resistance to chemotherapy is a principal problem in the treatment of small cell lung cancer (SCLC). We show here that SCLC is surrounded by an extensive stroma of extracellular matrix (ECM) at both primary and metastatic sites. Adhesion of SCLC cells to ECM enhances tumorigenicity and confers resistance to chemotherapeutic agents as a result of beta1 integrin-stimulated tyrosine kinase activation suppressing chemotherapy-induced apoptosis. SCLC may create a specialized microenvironment, and the survival of cells bound to ECM could explain the partial responses and local recurrence of SCLC often seen clinically after chemotherapy. Strategies based on blocking beta1 integrin-mediated survival signals may represent a new therapeutic approach to improve the response to chemotherapy in SCLC. 相似文献
95.
S.M.M. Karim S.S. Ratnam A.L. Kim K.C. Yeo H.T. Choo 《Prostaglandins & other lipid mediators》1982,23(2):257-263
16 phenoxy-ω-17,18,19,20 tetranor PGE2 methylsulfonylamide (Sulprostone) was used for termination of second trimester pregnancy in four groups of 30 patients. The drug was administered in intramuscular doses of either 0.5 mg four hourly or 1.0 mg 8 hourly. In two groups of 30 patients a medium size sterile laminaria was inserted into the cervical canal eight hours before the start of prostaglandin treatment. In the group treated with 1.0 mg sulprostone eight hourly, 96.7% of those with laminaria and 86.7% without laminaria aborted in respective mean times of 11.2 hrs and 17.5 hrs. All 30 patients (100%) in the laminaria group treated with 0.5 mg sulprostone four hourly aborted within 30 hours in a mean time of 10.4 hours compared with 26 patients (86.7%) in a mean time of 16.7 hours in the group without laminaria.One patient receiving 0.5 mg sulprostone four hourly (no laminaria) sustained a cervical tear requiring repair. The incidence of nausea, vomiting, diarrhoea, cold and shivering was low and similar in the four groups. 相似文献
96.
Siew Woh Choo Avirup Dutta Guat Jah Wong Wei Yee Wee Mia Yang Ang Cheuk Chuen Siow 《PloS one》2016,11(4)
Mycobacteria have been reported to cause a wide range of human diseases. We present the first whole-genome study of a Non-Tuberculous Mycobacterium, Mycobacterium sp. UM_CSW (referred to hereafter as UM_CSW), isolated from a patient diagnosed with bronchiectasis. Our data suggest that this clinical isolate is likely a novel mycobacterial species, supported by clear evidence from molecular phylogenetic, comparative genomic, ANI and AAI analyses. UM_CSW is closely related to the Mycobacterium avium complex. While it has characteristic features of an environmental bacterium, it also shows a high pathogenic potential with the presence of a wide variety of putative genes related to bacterial virulence and shares very similar pathogenomic profiles with the known pathogenic mycobacterial species. Thus, we conclude that this possible novel Mycobacterium species should be tightly monitored for its possible causative role in human infections. 相似文献
97.
In this paper, we propose an adversary model to facilitate forensic investigations of mobile devices (e.g. Android, iOS and Windows smartphones) that can be readily adapted to the latest mobile device technologies. This is essential given the ongoing and rapidly changing nature of mobile device technologies. An integral principle and significant constraint upon forensic practitioners is that of forensic soundness. Our adversary model specifically considers and integrates the constraints of forensic soundness on the adversary, in our case, a forensic practitioner. One construction of the adversary model is an evidence collection and analysis methodology for Android devices. Using the methodology with six popular cloud apps, we were successful in extracting various information of forensic interest in both the external and internal storage of the mobile device. 相似文献
98.
Moderate Weight Loss Improves Body Composition and Metabolic Function in Metabolically Unhealthy Lean Subjects 下载免费PDF全文
99.
K.H. Andy Choo 《American journal of human genetics》1997,61(6):1225-1233
100.
P. Y. Jui T. M. Choo K. M. Ho T. Konishi R. A. Martin 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1997,94(5):549-556
A study was conducted on a two-row/six-row cross of barley to (1) determine the yield potential, (2) detect epistasis and
genetic correlations, (3) estimate the heritabilities of six agronomic traits, and (4) study the effect of the V locus on the agronomic traits in the barley cross. The effects of five other marker loci (Re2, s, R, Est1, and Est5) on the six agronomic traits were also studied. One hundred and ninety doubled-haploid (DH) lines were derived from a ‘Leger’/CI9831
cross using the bulbosum method. The DH lines and the two parents were tested for grain yield, test weight, seed weight, plant
height, lodging, and heading/maturity at two locations in Eastern Canada in 1993. Additive×additive epistasis and genetic
correlations were detected for some of the agronomic traits. Many of the heritability estimates were high; however, significant
progress in yield improvement would be difficult to achieve because of a low mean yield of the DH lines. Under the growing
conditions in Eastern Canada, six-row lines outyielded two-row by 20–27%. Six-row lines, however, were associated with low
test weight, low seed weight, and severe lodging. Some two-row lines yielded higher than the two-row parent CI9831, but none
of the six-row lines yielded higher than the six-row parent ‘Leger’. The R, s, and Est5 loci were associated with the six agronomic traits, but the Est1 locus was apparently not associated with the agronomic traits. The effect of the Re2 locus was probably due to its close linkage with the V locus. Further studies are needed to determine if superior six-row lines can be developed from two-row/six-row crosses.
Received: 19 September 1996 / Accepted: 18 October 1996 相似文献