全文获取类型
收费全文 | 15956篇 |
免费 | 1663篇 |
国内免费 | 1998篇 |
出版年
2024年 | 69篇 |
2023年 | 270篇 |
2022年 | 543篇 |
2021年 | 862篇 |
2020年 | 626篇 |
2019年 | 798篇 |
2018年 | 662篇 |
2017年 | 541篇 |
2016年 | 709篇 |
2015年 | 1046篇 |
2014年 | 1225篇 |
2013年 | 1270篇 |
2012年 | 1501篇 |
2011年 | 1378篇 |
2010年 | 889篇 |
2009年 | 800篇 |
2008年 | 916篇 |
2007年 | 810篇 |
2006年 | 743篇 |
2005年 | 657篇 |
2004年 | 589篇 |
2003年 | 599篇 |
2002年 | 515篇 |
2001年 | 298篇 |
2000年 | 247篇 |
1999年 | 191篇 |
1998年 | 123篇 |
1997年 | 106篇 |
1996年 | 94篇 |
1995年 | 63篇 |
1994年 | 97篇 |
1993年 | 53篇 |
1992年 | 53篇 |
1991年 | 39篇 |
1990年 | 40篇 |
1989年 | 33篇 |
1988年 | 30篇 |
1987年 | 22篇 |
1986年 | 18篇 |
1985年 | 32篇 |
1984年 | 13篇 |
1983年 | 15篇 |
1982年 | 15篇 |
1981年 | 3篇 |
1979年 | 4篇 |
1978年 | 2篇 |
1977年 | 2篇 |
1973年 | 2篇 |
1971年 | 2篇 |
1961年 | 1篇 |
排序方式: 共有10000条查询结果,搜索用时 15 毫秒
41.
本文论证了菲波纳斯(Fibonacci)级数与菊花花冠结构的关系,发现了花冠的通用数学模式:花冠(数目)=(5×倍数)十F_n(F_(n-1)+F_(n-2)) 相似文献
42.
几种细胞分裂素诱导石竹外植体直接再分化成花芽的机理 总被引:4,自引:0,他引:4
用MS+2,4-D0.1mg/L为基本培养基,分别附加不同浓度的ZT、BA和KT,对石竹的叶片、茎和花等外植体直接再分化成花芽的试验表明:叶片的外植体在有BA时可再分化出只有红色花瓣的不正常花芽;在含ZT的培养基中再分化出具有1~2片叶或无叶的花芽,其花有两性花,雌花和仅单个雌蕊的不正常花,两性花可发育成结籽的果实;KT未能诱导花芽分化。茎外植体,3种CTK都不能诱导出花芽。花等外植体仅在KT3mg/L的培养中再分化出无叶的不正常花芽,ZT和BA的处理均不能诱导出花芽。 相似文献
43.
Structures of wild-type and mutant signal sequences of Escherichia coli ribose binding protein. 总被引:2,自引:1,他引:1
下载免费PDF全文
![点击此处可从《Biophysical journal》网站下载免费的PDF全文](/ch/ext_images/free.gif)
The structure of a chemically synthesized 25-residue-long functional signal peptide of Escherichia coli ribose binding protein was compared with that of a nonfunctional mutant-signal peptide using circular dichroism and two-dimensional 1H NMR in solvents mimicking the amphiphilic environments. The functional peptide forms an 18-residue-long alpha-helix starting from the NH2-terminal region and reaching to the hydrophobic stretch in a solvent consisting of 10% dimethylsulfoxide, 40% water, and 50% trifluoroethanol (v/v). The nonfunctional mutant peptide, which contains a Pro at position 9 instead of a Leu in the wild-type peptide, does not have any secondary structure in that solvent but forms a 12-residue-long alpha-helix within the hydrophobic stretch in water/trifluoroethanol (50:50, v/v) solvent. It seems that the Pro-9 residue in the nonfunctional peptide disturbs the helix propagation from the hydrophobic stretch to the NH2-terminal region. Because both of these peptides have stable helices within the hydrophobic stretch, it may be concluded that the additional 2 turns of the alpha-helix in the NH2-terminal region of the wild-type signal peptide is important for its function. 相似文献
44.
The infectivities of Steinernema carpocapsae, S. glaseri, S. scapterisci, and Heterorhabditis bacteriophora to Japanese beetle larvae, Popillia japonica, and house cricket adults, Acheta domesticus, were compared using external exposure and hemocoelic injection. Only H. bacteriophora and S. glaseri caused high P. japonica mortality after external exposure. When nematodes were injected, P. japonica had a strong encapsulation and melanization response to all species except S. glaseri. Heterorhabditis bacteriophora and S. carpocapsae were able to overcome the immune response, but S. scapterisci was not. All species except S. scapterisci were able to kill and reproduce within the host. Only S. scapterisci and S. carpocapsae caused A. domesticus mortality after external exposure. When nematodes were injected, A. domesticus had a strong immune response to all species except S. scapterisci. Steinernema carpocapsae effectively overcame the strong immune response and caused high host mortality, but S. glaseri and H. bacteriophora did not. Steinernema scapterisci caused high host mortality and reproduced, S. glaseri and H. bacteriophora caused low host mortality but only S. glaseri reproduced, and S. carpocapsae was able to kill the host but reproduced poorly. Most (ca. 90%) of the S. carpocapsae in the hemocoel of P. japonica became encapsulated and melanized within 8 hours postinjection. The symbiotic bacterium, Xenorhabduf nematophilus, was often released before this encapsulation and melanization. 相似文献
45.
作者在武陵山地区的鱼类寄生线虫调查工作中,发现一种毛细线虫,经研究,为一新种,定名为岁新毛细线虫,新种(Neocapillariaphoxinisp.nov.)。根据其特征,为此建立一新属──新毛细线虫属。 相似文献
46.
武陵山地区鱼类寄生杆咽属线虫两新种 总被引:1,自引:0,他引:1
本文报道在武陵山地区的白甲鱼[(Varicorkinus(Onychostoma)simus(SauvageetDabry)]和沪溪直口峻(RectorisluxiensisWuetYao)中发现的两种杆咽属(Rhabdochona)线虫新种,定名为白甲鱼杆咽线虫,新种(RhabdOchonaonyrkostomisp.nov.)和短咽杆咽线虫,新种(RhabdOchonabrevichonasp.nov.),并对两新种进行了测量和详细的描述。 相似文献
47.
野生大豆种子蛋白含量差异的生理及结构基础的探讨 总被引:1,自引:0,他引:1
利用SDS-聚丙烯酰胺凝胶电泳、电子显微镜、蛋白及酰脲含量测定等技术,对高蛋白含量(50.7% )的50359 和低蛋白含量(40.8% )的50305 两个野生大豆在种子发育过程中贮藏蛋白积累的速率、蛋白组分合成的起始时间、蛋白体发育的进程以及幼茎的酰脲含量进行了比较研究。结果表明:野生大豆50359的高蛋白含量是与其种子发育过程中较高的植株酰脲含量、较早较快的贮藏蛋白合成及积累速率,液泡中高效的蛋白贮藏方式以及蛋白体在子叶细胞中占有较大体积相联系的 相似文献
48.
水平回转对水稻幼苗叶细胞的影响 总被引:9,自引:0,他引:9
对在模拟微重力装置上回转14 天的水稻幼苗叶细胞进行了亚显微形态、电子探针和细胞酶化学研究。发现叶细胞质膜上Ca2+ -ATP酶活性消失,膜内钙总量上升、膜外钙总量下降,细胞骨架变得疏松,细胞壁变薄并凹凸不平。叶绿体的基粒和线粒体的内嵴亦有部分变化。其变化机制,首先是细胞质膜上Ca2+ -ATP酶活性消失,膜上钙泵停止工作,跨膜钙浓度差减小,膜内钙浓度上升,微管、微丝聚合受阻,细胞骨架疏松,分泌泡移动失去导向,从而导致细胞壁变薄等状态 相似文献
49.
几种濒危植物及其近缘类群总DNA的提取与鉴定 总被引:122,自引:0,他引:122
用低pH 介质,高盐沉淀蛋白质方法成功地从银杉(Cathaya argyrophylla Chun etKuang)、矮牡丹(Paeonia suffruticosa var. spontanea)、南川升麻(Cim icifuga nanchuanensisHsiao)、裂叶沙参(Adenophora lobophylla)的同属种泡沙参(A. potaninii)等植物中提取和部分纯化了细胞总DNA,并对其产率、质量和纯度作了鉴定。此方法的关键是用了一个低pH提取介质,它能有效防止组织破碎及沉淀大量材料时的电离化作用及酚化合物的进一步氧化。所得DNA 不需经氯化铯梯度离心或柱层析,直接可用于限制性片断长度多态性(RFLP)及随机扩增的DNA多态性(RAPD)等分子水平的遗传标记。为检测濒危植物的遗传多样性提供了一套迅速、简便和可靠的技术方案 相似文献
50.
Shukuro Araki Shigehiro Yi Tatsufumi Murakami Susumu Watanabe Shinichi Ikegawa Kiyoshi Takahashi Ken-ichi Yamarnura 《Molecular neurobiology》1994,8(1):15-23
To analyze the pathologic processes of amyloid deposition in type I familial amyloidotic polyneuropathy (FAP), mice were made
transgenic by introducing the human mutant transthyretin (TTR) gene(MT-hMet 30). An inbred strain of mouse, C57 BL/6, was
chosen. Transgenic mice were killed using ether anesthesia at 3-mo intervals up to 24 mo after birth. In these transgenic
mice, amyloid deposition started in the gastrointestinal tract, cardiovascular system, and kidneys and extended to various
other organs and tissues with advancing age. The pattern of amyloid deposition was similar to that observed in human autopsy
cases of FAP, except for its absence in the choroid plexus and in the peripheral and autonomic nervous systems.
We extracted the amyloid fibrils from kidneys of these mice with a human mutant TTR gene and analyzed them immunochemically
and electronmicroscopically. Deposited amyloid was shown to be composed of human mutant TTR and mouse serum amyloid P component.
Amyloid fibril from transgenic mice was morphologically and immunohistochemically similar to that of human FAP.
The most striking pathologic feature of the transgenic mice was the absence of amyloid deposition in the peripheral and autonomic
nervous tissues. Thus, other intrinsic factors may be involved in amyloid deposition in the nervous tissues of human FAP. 相似文献