Fungal diversity on submerged wood in a tropical stream and an artificial lake

Two collections of submerged wood were made from the Mushroom Research Centre in northern Thailand. One collection comprising 100 samples was made from an artificial lake, which had been made by damming a stream running through a secondary forest. The other collection comprising 90 samples was made from an adjacent non dammed shallow stream running through a similar forest. A total of 68 fungal taxa were recorded during the study and the Shannon–Weiner index (H′) was applied to evaluate the diversities of freshwater fungi. Sørensen’s index (S′) was calculated to evaluate the similarity of different fungal communities. The variation of freshwater fungi on submerged wood between lentic and lotic habitat is discussed and compared with previous studies. A dramatic decrease in species richness and diversity, with significantly changed species composition were observed in the artificial lake as compared to the non dammed stream.     

Use of a new strategy to isolate and characterize 436 Drosophila cDNA clones corresponding to RNAs detected in adult heads but not in early embryos

We describe a new strategy for producing tissue-specific cDNA libraries and subsequently identifying tissue-specific clones. This method was used to screen for cDNA clones corresponding to RNAs expressed in the Drosophila head that cannot be detected in the early embryo. RNA blots were used to assess the spatial and temporal patterns of expression of these RNAs. The ensemble of 436 head-not-embryo clones identified roughly 700 distinct RNAs that are differentially expressed in the Drosophila head. The RNA expression patterns can be classified into five major categories. it is argued that this ensemble of clones represents a large fraction of all genes differentially expressed in the adult head, but not detected in the early embryo. Many of these genes are likely to encode eye- and nervous system-specific products.     

Studies on the amphisphaeriales I. The clypeosphaeriaceae

The Clypeosphaeriaceae is retained to include sixteen genera.Apioclypea, Ceratostomella, Clypeophysalospora, Clypeosphaeria, Crassoascus, Jobellisia, Oxydothis andUrosporella are described and illustrated, whileApiorhynchostoma, Brunneiapiospora, Capsulospora, Duradens, Frondispora, Leiosphaerella, Pseudovalsaria andStereosphaeria are discussed. Genera in the Clypeosphaeriaceae are distinguished from those in, the Amphisphaeriaceae (sensu stricto). Their ascomata are immersed and typically clypeate. Asci are 8-spored, unitunicate, cylindrical, with a J+ or J−, apical ring. Ascospores are usually ellipsoidal or cylindrical, brown or hyaline, unicellular, pseudoseptase or septate, at times with appendages or germ pores. The Clypeosphaeriaceae is considered a member of the Amphisphaeriales and the differences between the Clypeosphaeriaceae and Amphisphaeriaceae are discussed. We are unsure if the Clypeosphaeriaceae as we define it is monophyletic.     

Morphometry of the coronary microvasculature of the canine left ventricle

The objective of this study was to test for the presence of transmural gradients of various components of the coronary microvasculature of the canine left ventricle. In order to achieve study objectives, the heart and coronary circulation were fixed in a reproducible state of myocardial and vascular tone (diastolic cardiac arrest and maximal coronary vasodilation). Morphometric methods which treat the coronary microvasculature as anisotropically arranged structures were applied for quantitative structural analysis. Eight dog hearts were fixed with a glutaraldehyde-cacodylate-buffered fixative by retrograde perfusion of the aorta with the heart in diastolic arrest and with maximal coronary vasodilation. Tissue samples were taken from areas near to the anterior and posterior papillary muscles from the subendocardium, subepicardium, and intermediate transmural locations. Morphometric results showed a homogeneously arranged array of microvascular and myocardial components with no significant differences in any of the primary morphometric measurements, down to the ultrastructural level, in myocytes relative to transmural location. The results suggest that transmural differences in coronary blood flow are not due to transmural structural differences but rather are due to physiological regulatory mechanisms of coronary blood flow. Further, the results indicate that failure to correct for anisotropy of myocardial structures can lead to erroneous conclusions concerning the structural basis of function in the heart.     

The influence of various steroids on the binding of methyltrienolone (R1881) to human placenta: evidence for a second steroid-binding site which stabilizes the R1881 binding site

Specificity studies of the binding of R1881 to crude placental homogenate gave surprising results in that certain steroids increased the binding of [3H]R1881 rather than displacing it. While data for 'competing', i.e. displacing, steroids were similar to those reported by other authors, there have been no previous reports of increased binding due to added steroids. This increased binding was due mainly to an increase in capacity (about 10-fold). These data suggest that the placental steroid-binding protein is unusual in that there is a second steroid-binding site whose occupancy increases the stability of the protein, thereby increasing its capacity to bind R1881.     

Anamorphic fungi from freshwater habitats in China: Dictyosporium tetrasporum and Exserticlava yunnanensis spp. nov., and two new records for Pseudofuscophialis lignicola and Pseudobotrytis terrestris

This article describes two new anamorphic fungi from freshwater habitats, Dictyosporium tetrasporum sp. nov. and Exserticlava yunnanensis sp. nov., based on morphological characters. Both species are illustrated with light micrographs and compared with similar taxa. Pseudofuscophialis lignicola and Pseudobotrytis terrestris are reported as new records from freshwater habitats.