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991.
Ahn HJ Kim YS Lee CH Cho EW Yoo HS Kim SH Ko JH Kim SJ 《Journal of biotechnology》2011,151(2):225-230
Aberrant glycosylation of human tissue inhibitor of metalloproteinase-1 (TIMP-1) by N-acetylglucosaminyltransferase-V (GnT-V) was previously reported to be related to cancer progression. Here, we report on the antibodies recognizing the structural features initiated by an addition of N-linked β(1,6)-N-acetylglucosamine (GlcNAc) branch by GnT-V on TIMP-1. Two glycoforms of TIMP-1, TIMP1-L produced in Lec4 cells without GnT-V activity and TIMP1-B in GnT-V overexpressing transfectant cells, were purified from culture supernatant and used for generation of antibodies. TIMP1-L was injected in the left hind footpad of mice as decoy and TIMP1-B in the right hind footpad as immunogen. Phage-displayed scFv library was constructed from the B cells retrieved from the right popliteal lymph nodes and subjected to panning and screening. Phage ELISA of individual clones revealed the scFv clones with preferential binding activity to TIMP1-B, and they were converted into an scFv-Fc format for further characterization of binding specificity. Glycan binding assay of an antibody, 1-9F, revealed its differential specificity toward an extension of glycan structure initiated with β(1,6)-GlcNAc linkage and terminally decorated with a sialic acid. This study demonstrates feasibility of a new strategy combining decoy immunization with phage display for the efficient generation of antibodies tracking down structural features of different glycoforms. 相似文献
992.
Choi JH Yoo KY Lee CH Yi SS Yoo DY Seong JK Yoon YS Hwang IK Won MH 《Cellular and molecular neurobiology》2011,31(2):285-292
N-methyl-d-aspartate receptor (NR) is involved in activity-dependent synaptic plasticity, such as associative long-term potentiation,
and in related central functions, such as learning and memory. In this study, we observed effects of treadmill exercise on
NR1 and doublecortin (DCX, a marker for neuroblast differentiation) in the subgranular zone of the dentate gyrus (DG). At
6 weeks of age, rats were put on a treadmill with or without running for 1 h/day for 5 consecutive days at 22 m/min for 5 weeks.
Exercise increased NR1 immunoreactivity and protein level in the hippocampus. To identify the correlations between NR and
neuroblasts, we intraperitoneally administered a NR antagonist, MK-801, to the exercised rats. MK-801 treatment reduced NR1
protein level in the hippocampus of the exercised rats. In addition, in the MK-801-treated group, the number of DCX cells
was significantly decreased in the subgranular zone of the DG. These results suggest that NR may be one of the important factors
that modulate neuroblast differentiation during exercise in rats. 相似文献
993.
Lee CH Yoo KY Choi JH Park JH Kim DH Park JH Hwang IK Cho JH Kim YM Won MH 《Cellular and molecular neurobiology》2011,31(3):449-457
In this study, the authors examined the difference of phosphorylated extracellular signal-regulated kinase 1/2 (pERK1/2) in
the hippocampal CA1 region (CA1) between adult and aged gerbils after 5 min of transient cerebral ischemia. Delayed neuronal
death in the CA1 of the aged group was much slower than that in the adult group after ischemia/reperfusion (I/R). pERK1/2
immunoreaction was observed in the CA1 region of the sham-operated adult gerbil. pERK1/2 immunoreactivity and protein levels
in the ischemic CA1 region of the adult group were markedly increased 4 days after I/R, and then reduced up to 10 days after
I/R. In contrast, pERK1/2 immunoreaction was hardly detected in the CA1 region of sham-operated aged gerbils, and the immunoreactivity
increased from 1 day after the ischemic insult, and still observed until 10 days post-ischemia. In addition, pERK1/2-immunoreaction
was expressed in astrocytes in the ischemic CA1 region: The expression in the ischemia-operated aged gerbils was later than
that in the ischemia-operated adult gerbils. These results indicate that different patterns of ERK1/2 immunoreactivity may
be associated with different processes of delayed neuronal death in adult and aged animals. 相似文献
994.
Various environmental oxidative stresses are sensed by redox-sensitive regulators through cysteine thiol oxidation or modification. A few zinc-containing anti-sigma (ZAS) factors in actinomycetes have been reported to respond sensitively to thiol oxidation, among which RsrA from Streptomyces coelicolor is best characterized. It forms disulfide bonds upon oxidation and releases bound SigR to activate thiol oxidative stress response genes. Even though numerous ZAS proteins exist in bacteria, features that confer redox sensitivity to a subset of these have been uncharacterized. In this study, we identified seven additional redox-sensitive ZAS factors from actinomycetes. Comparison with redox-insensitive ZAS revealed characteristic sequence patterns. Domain swapping demonstrated the significance of the region K(33)FEHH(37)FEEC(41)SPC(44)LEK(47) that encompass the conserved HX(3)CX(2)C (HCC) motif. Mutational effect of each residue on diamide responsive induction of SigR target genes in vivo demonstrated that several residues, especially those that flank two cysteines (E39, E40, L45, E46), contribute to redox sensitivity. These residues are well conserved among redox-sensitive ZAS factors, and hence are proposed as redox-determinants in sensitive ZAS. H37A, C41A, C44A and F38A mutations, in contrast, compromised SigR-binding activity significantly, apparently affecting structural integrity of RsrA. The residue pattern around HCC motif could therefore serve as an indicator to predict redox-sensitive ZAS factors from sequence information. 相似文献
995.
Lee CH Yoo DY Park OK Park JH Yi SS Yoon YS Won MH Hwang IK 《Neurochemical research》2011,36(10):1767-1775
Newly generated neurons in the dentate gyrus differentiate into mature granule cells. In the present study, we observed the effects of adrenalectomy (ADX) and corticosterone replacement therapy (CRT) on cell death, cell proliferation and neuroblast differentiation in the subgranular zone of the hippocampal dentate gyrus (SZDG). For this, the animals received vehicle or CRT after ADX, and were sacrificed 5 or 42 days later. Plasma corticosterone levels were very low in the adrenalectomized groups, whereas CRT after ADX significant increased serum corticosterone levels at 42 days, not 5 days, after ADX. ADX induced some neuronal damage in the dentate gyrus at 5 days post-ADX. CRT did not significantly reduce the neuronal damage at 5 days post-ADX; however, neuronal damage was not shown at 42 post-ADX with CRT. Ki67 (a marker for cell proliferation) and doublecortin (DCX, a marker for neuronal differentiation) immunoreaction was detected in the SZDG. ADX transiently increased cell proliferation and neuroblast differentiation 5 days after ADX, not 42 days, after ADX, and the CRT 42 days after ADX prominently decreased cell proliferation and neuroblast differentiation in the dentate gyrus. These results suggest that adrenal corticosteroid hormone is not essential for cell proliferation and neuroblast differentiation in long-term period after ADX. 相似文献
996.
Hwang IK Yi SS Yoo KY Park OK Yan B Song W Won MH Yoon YS Seong JK 《Neurochemical research》2011,36(8):1526-1532
In the present study, we investigated the effects of treadmill exercise in early and chronic diabetic stages on parvalbumin
(PV) immunoreactivity in the subgranular zone of the dentate gyrus of Zucker diabetic fatty (ZDF) and its lean control rats
(ZLC). To investigate the effects, ZLC and ZDF rats at 6 or 23 weeks of age were put on a treadmill with or without running
for 1 h/day/5 consecutive days at 16–22 m/min for 5 weeks or 12–16 m/min for 7 weeks, respectively. Physical exercise in pre-diabetic
rats prevented onset of diabetes, while exercise in rats at chronic diabetic stage significantly reduced blood glucose levels.
In addition, physical exercise in the pre-diabetic rats significantly increased PV immunoreactive fibers in the strata oriens
and radiatum of the CA1-3 region and in the polymorphic and molecular layers of the dentate gyrus compared to that in sedentary
controls. However, in rats at chronic stages, PV immunoreactivity was slightly increased in the CA1-3 region as well as in
the dentate gyrus compared to that in the sedentary controls. These results suggest that physical exercise has differential
effects on blood glucose levels and PV immunoreactivity according to diabetic stages. Early exercise improves diabetic phenotype
and PV immunoreactive fibers in the rat hippocampus. 相似文献
997.
Lee HJ Choi JH Ahn JH Lee CH Yoo KY Hwang IK Kim JS Kim C Lee YL Shin HC Won MH 《Neurochemical research》2011,36(3):435-442
We investigated distribution and age-related changes in two isoforms of GABA synthesizing enzymes, glutamic acid decarboxylase (GAD) 65 and 67, in the lumbar levels (L(5)-L(6)) of the dog spinal cord. Male German shepherds were used at 1-2 years (young adult dogs) and 10-12 years (aged dogs) of age. GAD65 immunoreaction was observed in neuropil, not in cell bodies, in all laminae of the adult lumbar spinal cord: Many punctate GAD65-immunoreactive structures were shown in all laminae. The density of GAD65 immunoreactive structures was highest in laminae I-III, and lowest in lamina VII. In the aged dog, the distribution pattern of GAD65 immunoreactivity was similar to that in the adult dog; however the density of GAD65-immunoreactive structures and its protein levels were significantly increased in the aged lumbar spinal cord. GAD67 immunoreaction in the adult dog was also distributed in all laminae of the lumbar spinal cord like GAD65; however, we found that small GAD67-immunoreactive cell bodies were observed in laminae II, III and VIII. In the aged dogs, GAD67 immunoreactivity and its protein levels were also increased compared to those in the adult group. In conclusion, our results indicate that the distribution of GAD65-immunoreactive structures is different from GAD67-immunoreactive structures and that their immunoreactivity in the aged dogs is much higher than the adult dogs. 相似文献
998.
Park JH Yoo KY Lee CH Kim IH Shin BN Choi JH Park JH Hwang IK Won MH 《Neurochemical research》2011,36(6):1037-1045
Stress leads to changes in homeostasis and internal balance of the body and is known to be one of important factors in the
development of several diseases. In the present study, we investigated changes in glucocorticoid receptor (GR) and ionized
calcium-binding adapter molecule 1 (Iba-1) immunoreactivity in the adult and aged gerbil hippocampus after chronic restraint
stress. Serum corticosterone level was much higher in both the stress-groups than the control groups. No neuronal death was
found in all hippocampal subregions of the adult and aged gerbil after chronic restraint stress. GR immunoreactivity was decreased
in both the adult and aged groups after repeated restraint stress; however, GR immunoreactivity in the adult-stress-group
was decreased much more than that in the aged-stress-group. Iba-1 immunoreactive microglia were hypertrophied and activated
in the adult group after repeated restraint stress; in the aged-stress-group, there was no any significant change in Iba-1
immunoreactive microglia. In brief, level of GR, not Iba-1, in the hippocampus was much decreased in the adult gerbil compared
to the aged gerbil following chronic restraint stress. 相似文献
999.
Regulation of intestinal stem cell proliferation by human methyl-CpG-binding protein-2 in Drosophila
Lee SH Kim IJ Kim JG Park JS Kim YS Yamaguchi M Kim CM Yoo MA 《Cell structure and function》2011,36(2):197-208
Recent studies have suggested the involvement of epigenetic factors such as methyl-CpG-binding protein-2 (MeCP2) in tumorigenesis. In addition, cancer may represent a stem cell-based disease, suggesting that understanding of stem cell regulation could provide valuable insights into the mechanisms of tumorigenesis. However, the function of epigenetic factors in stem cell regulation in adult tissues remains poorly understood. In the present study, we investigated the role of human MeCP2 (hMeCP2), a bridge factor linked to DNA modification and histone modification, in stem cell proliferation using adult Drosophila midgut, which appears to be an excellent model system to study stem cell biology. Results show that enterocyte (EC)-specific expression of hMeCP2 in adult midgut using an exogenous GAL4/UAS expression system induced intestinal stem cell (ISC) proliferation marked by staining with anti-phospho-histone H3 antibody and BrdU incorporation assays. In addition, hMeCP2 expression in ECs activated extracellular stress-response kinase signals in ISCs. Furthermore, expression of hMeCP2 modulated the distribution of heterochromatin protein-1 in ECs. Our data suggests the hypothesis that the expression of hMeCP2 in differentiated ECs stimulates ISC proliferation, implying a role of MeCP2 as a stem cell regulator. 相似文献
1000.
Hee-Hang Kim Jong-Hyun Jung Dong-Ho Seo Suk-Jin Ha Sang-Ho Yoo Chung-Ho Kim Cheon-Seok Park 《World journal of microbiology & biotechnology》2011,27(12):2851-2856
Trehalose (α-d-glucopyranosyl α-d-glucopyranoside) is an important non-reducing disaccharide used in the food industry due to its mild sweetness (45% that
of sucrose), low cariogenicity, high glass transition temperature, low hygroscopicity, and protein protection properties.
In this study, we accomplished the production of trehalose from sucrose as a sole substrate using a novel dual-enzyme system,
in which amylosucrase (ASase) and maltooligosyltrehalose synthase-trehalohydrolase (MTSH) fusion enzyme were employed. The
biotransformation of sucrose to trehalose was confirmed by high-performance anion-exchange chromatography (HPAEC) analysis.
Trehalose was successfully produced by both simultaneous and sequential methods by using ASase and MTSH. A higher trehalose
production yield (3.15 ± 0.83 mM trehalose/20 mM sucrose) was observed in the sequential method than the simultaneous method
(1.43 ± 0.14 mM trehalose/20 mM sucrose), indicating that the production of maltooligosaccharides from sucrose by ASase was
an important step in the biosynthesis of trehalose. 相似文献