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Cheremnykh E. G. Osipov A. V. Starkov V. G. Trang Nguyen Thi Thuy Khoa Nguyen Cuu Anh Hoang Ngoc Dung Le Tien Tsetlin V. I. Utkin Yu. N. 《Doklady. Biochemistry and biophysics》2022,503(1):98-103
Doklady Biochemistry and Biophysics - To search for compounds with antiprotozoal activity, effects of snake venoms on the ciliates Tetrahymena pyriformis was studied. T. pyriformis from subkingdom... 相似文献
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Dong Tu Nguyen Tuan Cuong Ngo Huy Hoang Tran Thi Phuong Lan Nguyen Binh Minh Nguyen Kouichi Morita & Masahiko Ehara 《FEMS microbiology letters》2009,298(1):37-43
Autoagglutinable strains of Vibrio cholerae O1 (seven nonfimbriate strains and one fimbriate strain) were transformed to obtain resistance to ampicillin. Two distinct mechanisms were found in these strains. One was operating in nonfimbriate strains by reducing OmpU protein production and the other was operating in a fimbriate strain (Bgd17) by newly overproducing cpxP protein. The twitching motility in the fimbriate Bgd17 strain disappeared depending on the production of cpxP protein, suggesting that fimbriation of V. cholerae O1 is controlled by a two-component signal transduction system. 相似文献
855.
Renal cytosolic extracts from rats of different ages and mononephrectomized rats were incubated with gamma-[32P]ATP and analysed by high resolution two-dimensional electrophoresis and autoradiography. Extracts from new-born and young rats showed a great number of phosphorylated proteins migrating between the origin and Mr 52,000. Among these proteins, the group co-migrating with phosphorylase b (Mr 97,000) was particularly evident in new-born and days-old rats. In extracts from mature rats, other proteins of lower molecular weight, particularly those migrating between Mr 60,000 and 44,000, became intensely phosphorylated. The number and intensity of phosphorylated proteins from extracts of normal and nephrectomized rats, however, did not vary. Activity of cAMP-dependent protein kinase and [3H]cAMP binding was also modified during neonatal development but not in compensatory renal growth. Since cAMP-PK and protein phosphorylation are known to be regulated in response to hormonal stimulations, these results may provide good indications for the understanding of hormonal involvement in kidney growth. 相似文献
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Son Hoang Minh Duc Hoang Minh Masuda Yoshimitsu Honjoh Ken-ichi Miyamoto Takahisa 《Applied microbiology and biotechnology》2018,102(23):10259-10271
Applied Microbiology and Biotechnology - Shiga toxin-producing Escherichia coli (STEC) O157:H7 and extended-spectrum beta-lactamase (ESBL) producing E. coli (ESBLEC) are important bacteria of... 相似文献
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Duc Hoang Bingyu Kuang George Liang Zhao Wang Seongkyu Yoon 《Biotechnology progress》2023,39(2):e3313
Therapeutic protein productivity and glycosylation pattern highly rely on cell metabolism. Cell culture medium composition and feeding strategy are critical to regulate cell metabolism. In this study, the relationship between toxic metabolic inhibitors and their nutrient precursors was explored to identify the critical medium components toward cell growth and generation of metabolic by-products. Generic CHO metabolic model was tailored and integrated with CHO fed-batch metabolomic data to obtain a cell line- and process-specific model. Flux balance analysis study was conducted on toxic metabolites cytidine monophosphate, guanosine monophosphate and n-acetylputrescine—all of which were previously reported to generate from endogenous cell metabolism—by mapping them to a compartmentalized carbon utilization network. Using this approach, the study projected high level of inhibitory metabolites accumulation when comparing three industrially relevant fed-batch feeding conditions one against another, from which the results were validated via a dose-dependent amino acids spiking study. In the end, a medium optimization design was employed to lower the amount of supplemented nutrients, of which improvements in critical process performance were realized at 40% increase in peak viable cell density (VCD), 15% increase in integral VCD, and 37% increase in growth rate. Tight control of toxic by-products was also achieved, as the study measured decreased inhibitory metabolites accumulation across all conditions. Overall, the study successfully presented a digital twin approach to investigate the intertwined relationship between supplemented medium constituents and downstream toxic metabolites generated through host cell metabolism, further elucidating different control strategies capable of improving cellular phenotypes and regulating toxic inhibitors. 相似文献
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Hung Le Dinh Nguyen Hoang Thi Trang Trang Vo Thi Dieu 《Journal of applied phycology》2021,33(3):1819-1824
Journal of Applied Phycology - The red alga Kappaphycus striatus “sacol” brown strain is an economically important species and extensively cultivated at Vanphong Bay, Vietnam. To... 相似文献