Economic threshold for soybean aphid (Hemiptera: Aphididae)

Soybean aphid, Aphis glycines Matsumura (Hemiptera: Aphididae), reached damaging levels in 2003 and 2005 in soybean, Glycine max (L.) Merrill, in most northern U.S. states and Canadian provinces, and it has become one of the most important pests of soybean throughout the North Central region. A common experimental protocol was adopted by participants in six states who provided data from 19 yield-loss experiments conducted over a 3-yr period. Population doubling times for field populations of soybean aphid averaged 6.8 d +/- 0.8 d (mean +/- SEM). The average economic threshold (ET) over all control costs, market values, and yield was 273 +/- 38 (mean +/- 95% confidence interval [CI], range 111-567) aphids per plant. This ET provides a 7-d lead time before aphid populations are expected to exceed the economic injury level (EIL) of 674 +/- 95 (mean +/- 95% CI, range 275-1,399) aphids per plant. Peak aphid density in 18 of the 19 location-years occurred during soybean growth stages R3 (beginning pod formation) to R5 (full size pod) with a single data set having aphid populations peaking at R6 (full size green seed). The ET developed here is strongly supported through soybean growth stage R5. Setting an ET at lower aphid densities increases the risk to producers by treating an aphid population that is growing too slowly to exceed the EIL in 7 d, eliminates generalist predators, and exposes a larger portion of the soybean aphid population to selection by insecticides, which could lead to development of insecticide resistance.     

Intertidal meiofaunal biodiversity with respect to different algal habitats: a test using phytal ostracodes from Southern California

Rocky intertidal algae harbor a diverse invertebrate meiofauna of arthropods, nematodes and other invertebrates. Despite its ecological importance, relatively little is known about the diversity and composition of this important component of intertidal biodiversity. In this study, we quantified species composition, abundance and distribution of ostracodes, an important group of phytal meiofauna, at two different intertidal areas in southern California. In total, we recovered 22 ostracode species from three different orders (16 podocopids, five myodocopids and one platycopid), nearly a quarter of which could not be assigned to existing taxa. The abundance of ostracodes differed significantly among algal types, with structurally complex algae bearing many more ostracodes per gram of algae than simple forms (blade-like algae and the surfgrass Phyllospadix). Although most ostracode species were recovered from multiple kinds of algae, different algae harbored distinct assemblages that could be discriminated statistically on the basis of relative abundances of ostracode species. This segregation of the ostracode fauna according to algal species is evident even over very short spatial scales (<1 m). Finally, ostracode samples from turf-forming algae were more species rich than samples from other kinds of macroalgae. Since turf-forming algae are easily damaged by human trampling, this component of ostracode biodiversity may be particularly vulnerable to anthropogenic impacts on the intertidal habitat. Electronic supplementary material The online version of this article (doi:) contains supplementary material, which is available to authorized users. Handling editor: K. Martens     

Essential bacterial functions encoded by gene pairs

To address the need for new antibacterials, a number of bacterial genomes have been systematically disrupted to identify essential genes. Such programs have focused on the disruption of single genes and may have missed functions encoded by gene pairs or multiple genes. In this work, we hypothesized that we could predict the identity of pairs of proteins within one organism that have the same function. We identified 135 putative protein pairs in Bacillus subtilis and attempted to disrupt the genes forming these, singly and then in pairs. The single gene disruptions revealed new genes that could not be disrupted individually and other genes required for growth in minimal medium or for sporulation. The pairwise disruptions revealed seven pairs of proteins that are likely to have the same function, as the presence of one protein can compensate for the absence of the other. Six of these pairs are essential for bacterial viability and in four cases show a pattern of species conservation appropriate for potential antibacterial development. This work highlights the importance of combinatorial studies in understanding gene duplication and identifying functional redundancy.     

Insulin controls subcellular localization and multisite phosphorylation of the phosphatidic acid phosphatase, lipin 1

Brain, liver, kidney, heart, and skeletal muscle from fatty liver dystrophy (fld/fld) mice, which do not express lipin 1 (lipin), contained much less Mg(2+)-dependent phosphatidic acid phosphatase (PAP) activity than tissues from wild type mice. Lipin harboring the fld(2j) (Gly(84) --> Arg) mutation exhibited relatively little PAP activity. These results indicate that lipin is a major PAP in vivo and that the loss of PAP activity contributes to the fld phenotype. PAP activity was readily detected in immune complexes of lipin from 3T3-L1 adipocytes, where the protein was found both as a microsomal form and a soluble, more highly phosphorylated, form. Fifteen phosphorylation sites were identified by mass spectrometric analyses. Insulin increased the phosphorylation of multiple sites and promoted a gel shift that was due in part to phosphorylation of Ser(106). In contrast, epinephrine and oleic acid promoted dephosphorylation of lipin. The PAP-specific activity of lipin was not affected by the hormones or by dephosphorylation of lipin with protein phosphatase 1. However, the ratio of soluble to microsomal lipin was markedly increased in response to insulin and decreased in response to epinephrine and oleic acid. The results suggest that insulin and epinephrine control lipin primarily by changing localization rather than intrinsic PAP activity.     

The microneme proteins EtMIC4 and EtMIC5 of Eimeria tenella form a novel, ultra-high molecular mass protein complex that binds target host cells

Eimeria tenella, in common with other parasitic protozoa of the phylum Apicomplexa, invades host cells using an actinomyosin-powered "glideosome" complex and requires the secretion of adhesive proteins from the microneme organelles onto the parasite surface. Microneme proteins of E. tenella include EtMIC4, a transmembrane protein that has multiple thrombospondin type I domains and calcium-binding epidermal growth factor-like domains in its extracellular domain, and EtMIC5, a soluble protein composed of 11 tandemly repeated domains that belong to the plasminogen-apple-nematode superfamily. We show here that EtMIC4 and EtMIC5 interact to form an oligomeric, ultrahigh molecular mass protein complex. The complex was purified from lysed parasites by non-denaturing techniques, and the stoichiometry was shown to be [EtMIC4](2):[EtMIC5](1), with an octamer of EtMIC4 bound non-covalently to a tetramer of EtMIC5. The complex is formed within the parasite secretory pathway and is maintained after secretion onto the surface of the parasite. The purified complex binds to a number of epithelial cell lines in culture. Identification and characterization of this complex contributes to an overall understanding of the role of multimolecular protein complexes in specific interactions between pathogens and their hosts during infection.     

Stable-isotope probing with multiple growth substrates to determine substrate specificity of uncultivated bacteria

Stable-isotope probing (SIP) has been used to determine which microorganisms in a complex environmental sample are capable of metabolizing a labeled substrate. We hypothesized that DNA-based stable-isotope probing with a combination of a (13)C-labeled carbon source and a second, unlabeled carbon source could be combined with analyses of the entire gradient of separated DNA to provide information concerning the utilization of a mixture of environmentally relevant compounds by uncultivated organisms. As a test of the method, we evaluated the response of a microbial community in a laboratory bioreactor treating contaminated soil to two polycyclic aromatic hydrocarbons (PAH). The compounds were added either individually as [U-(13)C]phenanthrene or [U-(13)C]pyrene, or as a mixture in which one was labeled with (13)C and the other was unlabeled. After ultracentrifugation of DNA extracted from a given incubation, fractions containing DNA enriched with varying levels of (13)C were examined by denaturing-gradient gel electrophoresis (DGGE) and by real-time quantitative PCR (qPCR) for 16S rRNA genes belonging to organisms in groups of bacteria previously associated with PAH degradation by single-compound SIP. Four groups of bacteria (three uncultivated) were followed in this study. Two of the uncultivated groups showed evidence for simultaneous or sequential utilization of both compounds while the remaining two appeared to assimilate carbon from only one of the compounds. DNA-based SIP therefore appears to be useful to evaluate the selectivity among substrates in a mixture by uncultivated microbes.