全文获取类型
收费全文 | 2622篇 |
免费 | 336篇 |
国内免费 | 2篇 |
出版年
2021年 | 26篇 |
2019年 | 28篇 |
2017年 | 27篇 |
2016年 | 33篇 |
2015年 | 44篇 |
2014年 | 74篇 |
2013年 | 93篇 |
2012年 | 109篇 |
2011年 | 93篇 |
2010年 | 82篇 |
2009年 | 75篇 |
2008年 | 107篇 |
2007年 | 115篇 |
2006年 | 99篇 |
2005年 | 104篇 |
2004年 | 111篇 |
2003年 | 72篇 |
2002年 | 87篇 |
2001年 | 79篇 |
2000年 | 79篇 |
1999年 | 62篇 |
1998年 | 52篇 |
1997年 | 42篇 |
1996年 | 33篇 |
1995年 | 30篇 |
1994年 | 30篇 |
1993年 | 26篇 |
1992年 | 65篇 |
1991年 | 64篇 |
1990年 | 56篇 |
1989年 | 65篇 |
1988年 | 51篇 |
1987年 | 47篇 |
1986年 | 40篇 |
1985年 | 49篇 |
1984年 | 44篇 |
1983年 | 36篇 |
1982年 | 32篇 |
1981年 | 27篇 |
1980年 | 27篇 |
1979年 | 31篇 |
1978年 | 27篇 |
1977年 | 25篇 |
1976年 | 35篇 |
1975年 | 31篇 |
1974年 | 26篇 |
1973年 | 24篇 |
1972年 | 39篇 |
1969年 | 25篇 |
1968年 | 24篇 |
排序方式: 共有2960条查询结果,搜索用时 15 毫秒
991.
Kuwabara A Backhaus A Malinowski R Bauch M Hunt L Nagata T Monk N Sanguinetti G Fleming A 《Plant physiology》2011,156(4):2196-2206
Understanding the relationship of the size and shape of an organism to the size, shape, and number of its constituent cells is a basic problem in biology; however, numerous studies indicate that the relationship is complex and often nonintuitive. To investigate this problem, we used a system for the inducible expression of genes involved in the G1/S transition of the plant cell cycle and analyzed the outcome on leaf shape. By combining a careful developmental staging with a quantitative analysis of the temporal and spatial response of cell division pattern and leaf shape to these manipulations, we found that changes in cell division frequency occurred much later than the observed changes in leaf shape. These data indicate that altered cell division frequency cannot be causally involved in the observed change of shape. Rather, a shift to a smaller cell size as a result of the genetic manipulations performed correlated with the formation of a smoother leaf perimeter, i.e. appeared to be the primary cellular driver influencing form. These data are discussed in the context of the relationship of cell division, growth, and leaf size and shape. 相似文献
992.
993.
994.
995.
Economic threshold for soybean aphid (Hemiptera: Aphididae) 总被引:9,自引:0,他引:9
Ragsdale DW McCornack BP Venette RC Potter BD MacRae IV Hodgson EW O'Neal ME Johnson KD O'Neil RJ DiFonzo CD Hunt TE Glogoza PA Cullen EM 《Journal of economic entomology》2007,100(4):1258-1267
Soybean aphid, Aphis glycines Matsumura (Hemiptera: Aphididae), reached damaging levels in 2003 and 2005 in soybean, Glycine max (L.) Merrill, in most northern U.S. states and Canadian provinces, and it has become one of the most important pests of soybean throughout the North Central region. A common experimental protocol was adopted by participants in six states who provided data from 19 yield-loss experiments conducted over a 3-yr period. Population doubling times for field populations of soybean aphid averaged 6.8 d +/- 0.8 d (mean +/- SEM). The average economic threshold (ET) over all control costs, market values, and yield was 273 +/- 38 (mean +/- 95% confidence interval [CI], range 111-567) aphids per plant. This ET provides a 7-d lead time before aphid populations are expected to exceed the economic injury level (EIL) of 674 +/- 95 (mean +/- 95% CI, range 275-1,399) aphids per plant. Peak aphid density in 18 of the 19 location-years occurred during soybean growth stages R3 (beginning pod formation) to R5 (full size pod) with a single data set having aphid populations peaking at R6 (full size green seed). The ET developed here is strongly supported through soybean growth stage R5. Setting an ET at lower aphid densities increases the risk to producers by treating an aphid population that is growing too slowly to exceed the EIL in 7 d, eliminates generalist predators, and exposes a larger portion of the soybean aphid population to selection by insecticides, which could lead to development of insecticide resistance. 相似文献
996.
997.
Rocky intertidal algae harbor a diverse invertebrate meiofauna of arthropods, nematodes and other invertebrates. Despite its
ecological importance, relatively little is known about the diversity and composition of this important component of intertidal
biodiversity. In this study, we quantified species composition, abundance and distribution of ostracodes, an important group
of phytal meiofauna, at two different intertidal areas in southern California. In total, we recovered 22 ostracode species
from three different orders (16 podocopids, five myodocopids and one platycopid), nearly a quarter of which could not be assigned
to existing taxa. The abundance of ostracodes differed significantly among algal types, with structurally complex algae bearing
many more ostracodes per gram of algae than simple forms (blade-like algae and the surfgrass Phyllospadix). Although most ostracode species were recovered from multiple kinds of algae, different algae harbored distinct assemblages
that could be discriminated statistically on the basis of relative abundances of ostracode species. This segregation of the
ostracode fauna according to algal species is evident even over very short spatial scales (<1 m). Finally, ostracode samples
from turf-forming algae were more species rich than samples from other kinds of macroalgae. Since turf-forming algae are easily
damaged by human trampling, this component of ostracode biodiversity may be particularly vulnerable to anthropogenic impacts
on the intertidal habitat.
Electronic supplementary material The online version of this article (doi:) contains supplementary material, which is available to authorized users.
Handling editor: K. Martens 相似文献
998.
Thomaides HB Davison EJ Burston L Johnson H Brown DR Hunt AC Errington J Czaplewski L 《Journal of bacteriology》2007,189(2):591-602
To address the need for new antibacterials, a number of bacterial genomes have been systematically disrupted to identify essential genes. Such programs have focused on the disruption of single genes and may have missed functions encoded by gene pairs or multiple genes. In this work, we hypothesized that we could predict the identity of pairs of proteins within one organism that have the same function. We identified 135 putative protein pairs in Bacillus subtilis and attempted to disrupt the genes forming these, singly and then in pairs. The single gene disruptions revealed new genes that could not be disrupted individually and other genes required for growth in minimal medium or for sporulation. The pairwise disruptions revealed seven pairs of proteins that are likely to have the same function, as the presence of one protein can compensate for the absence of the other. Six of these pairs are essential for bacterial viability and in four cases show a pattern of species conservation appropriate for potential antibacterial development. This work highlights the importance of combinatorial studies in understanding gene duplication and identifying functional redundancy. 相似文献
999.
Harris TE Huffman TA Chi A Shabanowitz J Hunt DF Kumar A Lawrence JC 《The Journal of biological chemistry》2007,282(1):277-286
Brain, liver, kidney, heart, and skeletal muscle from fatty liver dystrophy (fld/fld) mice, which do not express lipin 1 (lipin), contained much less Mg(2+)-dependent phosphatidic acid phosphatase (PAP) activity than tissues from wild type mice. Lipin harboring the fld(2j) (Gly(84) --> Arg) mutation exhibited relatively little PAP activity. These results indicate that lipin is a major PAP in vivo and that the loss of PAP activity contributes to the fld phenotype. PAP activity was readily detected in immune complexes of lipin from 3T3-L1 adipocytes, where the protein was found both as a microsomal form and a soluble, more highly phosphorylated, form. Fifteen phosphorylation sites were identified by mass spectrometric analyses. Insulin increased the phosphorylation of multiple sites and promoted a gel shift that was due in part to phosphorylation of Ser(106). In contrast, epinephrine and oleic acid promoted dephosphorylation of lipin. The PAP-specific activity of lipin was not affected by the hormones or by dephosphorylation of lipin with protein phosphatase 1. However, the ratio of soluble to microsomal lipin was markedly increased in response to insulin and decreased in response to epinephrine and oleic acid. The results suggest that insulin and epinephrine control lipin primarily by changing localization rather than intrinsic PAP activity. 相似文献
1000.
The microneme proteins EtMIC4 and EtMIC5 of Eimeria tenella form a novel, ultra-high molecular mass protein complex that binds target host cells 总被引:1,自引:0,他引:1
Periz J Gill AC Hunt L Brown P Tomley FM 《The Journal of biological chemistry》2007,282(23):16891-16898
Eimeria tenella, in common with other parasitic protozoa of the phylum Apicomplexa, invades host cells using an actinomyosin-powered "glideosome" complex and requires the secretion of adhesive proteins from the microneme organelles onto the parasite surface. Microneme proteins of E. tenella include EtMIC4, a transmembrane protein that has multiple thrombospondin type I domains and calcium-binding epidermal growth factor-like domains in its extracellular domain, and EtMIC5, a soluble protein composed of 11 tandemly repeated domains that belong to the plasminogen-apple-nematode superfamily. We show here that EtMIC4 and EtMIC5 interact to form an oligomeric, ultrahigh molecular mass protein complex. The complex was purified from lysed parasites by non-denaturing techniques, and the stoichiometry was shown to be [EtMIC4](2):[EtMIC5](1), with an octamer of EtMIC4 bound non-covalently to a tetramer of EtMIC5. The complex is formed within the parasite secretory pathway and is maintained after secretion onto the surface of the parasite. The purified complex binds to a number of epithelial cell lines in culture. Identification and characterization of this complex contributes to an overall understanding of the role of multimolecular protein complexes in specific interactions between pathogens and their hosts during infection. 相似文献