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In this paper we report the results obtained from experiments with a database of emotional speech in English in order to find the most important acoustic features to estimate Emotion Primitives which determine the emotional content on speech. We are interested in exploiting the potential benefits of continuous emotion models, so in this paper we demonstrate the feasibility of applying this approach to annotation of emotional speech and we explore ways to take advantage of this kind of annotation to improve the automatic classification of basic emotions.  相似文献   
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Montezuma quail (Cyrtonyx montezumae) inhabit oak (Quercus spp.)-juniper (Juniperus spp.) woodlands throughout Mexico and the southwestern United States. In Texas, USA, Montezuma quail occur in the Edwards Plateau and Trans-Pecos Mountains and Basins (Trans-Pecos), 2 ecoregions with contrasting juniper patterns. Ashe juniper (Juniperus ashei) dominates in the Edwards Plateau and has been increasing over decades, whereas alligator juniper (Juniperus deppeana) is a co-dominant in the Trans-Pecos and appears to have stable Montezuma quail populations. Our objectives were to compare between ecoregions the relative abundance and habitat use of Montezuma quail in relation to juniper and quantify the influence of juniper on key features of Montezuma quail habitat (grass height, grass cover, forb cover, and forb species richness). We conducted a study from March–August 2018‒2020 in the Edwards Plateau (Kinney and Edwards counties) and Trans-Pecos ecoregions (Jeff Davis County) to evaluate these objectives. We conducted call-back surveys to estimate relative abundance (number of detections/hr) of Montezuma quail and identify used locations. We collected vegetation data at a micro-scale (16-m) at used (n = 32–30 points) and random locations (n = 70–60 points) in each ecoregion. Relative abundance of Montezuma quail was considerably lower in the Edwards Plateau (0.06 ± 0.01 detections/survey hr; ± SE) than in the Trans-Pecos (1.10 ± 0.30 detections/survey hr). In addition, Montezuma quail selected areas of low Ashe juniper cover (<23% cover), density (<7 trees/80 m2), and height (<2 m) in the Edwards Plateau but selected areas of high alligator juniper cover (>18% cover), density (>4 trees/80 m2), and height (2–8 m) in the Trans-Pecos. Moreover, Ashe juniper cover had a significant, negative influence on herbaceous features, whereas alligator juniper exerted little to no influence. Our results suggest that these 2 juniper species have contrasting effects on Montezuma quail space use and their habitat, possibly as a result of the contrasting influence of these junipers on herbaceous understory. Creating habitat for Montezuma quail in the Edwards Plateau generally will involve the reduction of Ashe juniper and creating patches that possess juniper trees of small stature (<2 m) with low amounts of cover (<23%) and density (<7 trees/80 m2), whereas management in the Trans-Pecos will require site-specific assessments based on current alligator juniper influence. © 2021 The Wildlife Society.  相似文献   
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A novel hypothesis to better understand the evolution of gigantism in active marine predators and the diversity of body sizes, feeding strategies and thermophysiologies of extinct and living aquatic vertebrates is proposed. Recent works suggest that some aspects of animal energetics can act as constraining factors for body size. Given that mass-specific metabolic rate decreases with body mass, the body size of active predators should be limited by the high metabolic demand of this feeding strategy. In this context, we propose that shifts towards higher metabolic levels can enable the same activity and feeding strategy to be maintained at bigger body sizes, offering a satisfactory explanation for the evolution of gigantism in active predators, including a vast quantity of fossil taxa. Therefore, assessing the metabolic ceilings of living aquatic vertebrates and the thermoregulatory strategies of certain key extinct groups is now crucial to define the energetic limits of predation and provide quantitative support for this model.  相似文献   
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Background  

Silver nanoparticles have proven to exert antiviral activity against HIV-1 at non-cytotoxic concentrations, but the mechanism underlying their HIV-inhibitory activity has not been not fully elucidated. In this study, silver nanoparticles are evaluated to elucidate their mode of antiviral action against HIV-1 using a panel of different in vitro assays.  相似文献   
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The growth and botulinic toxin production of Clostridium argentinense G 89 HT in co-culture with Pseudomonas mendocina were studied using two culture systems with carbon sources of rapid and slow utilization. Growth with glucose in homogeneous co-culture presented a dual-phase progression, with the toxin produced in the slow-growing phase. The extended 50 h growth period of the second phase at low specific growth rate was attributed to the combined metabolization of glucose and a complex carbon source of the alginate type produced by P. mendocina under strongly reducing conditions. With dextrin, the co-culture grew at the lower specific growth rate (μ = 0.03 h−1) for a period lasting 80 h. This fully enhanced the production of toxin with a specific toxicity 2.5 times higher than with glucose in a homogeneous system and 10.7 higher than that of C. argentinense G 89 HT single culture. The heterogeneous co-culture obtained with a dialysis membrane physically separating both bacteria (thereby eliminating the metabolic interaction) produced the lowest levels of growth and toxin of all the cases analyzed.  相似文献   
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