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41.
采用反相高效液相色谱法和电喷雾质谱法对我国野生笃斯越橘中花青素组分进行了分离和分析.制备野生越橘花青素的粗提物,经大孔树脂柱层析得到花青素样品,其得率为0.72%.用香草醛法测得其总黄烷醇含量为95%.采用Alltima C18反相色谱柱(250 mm×4.6 mm,5 μm),以2 %甲酸水溶液(A)和2 %甲酸/甲醇溶液(B)作为流动相,分2个梯度对花青素组分进行洗脱,将其主要洗脱峰在电喷雾正离子模式下进行分析,根据质谱的准分子离子[M+H](m/z)检测其聚合度.结果表明,我国野生越橘花青素的提取物被展开成8个主要的洗脱峰,以峰面积计算,主要洗脱峰总含量达85%.经质谱分析,判断为2个单体,3个二聚体,1个三聚体,2个四聚体.结果表明,我国野生笃斯越橘中含有较丰富的花青素,低聚体是其中的主要成分.本文首次报道了我国大兴安岭野生笃斯越橘花青素的组成情况,对于开展其后续的结构、功能、质量检测以及生产开发研究都具有一定的参考价值. 相似文献
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Qu Z Yang Z Cui N Zhu G Liu C Xu H Chanchevalap S Shen W Wu J Li Y Jiang C 《The Journal of biological chemistry》2000,275(41):31573-31580
Ion channels play an important role in cellular functions, and specific cellular activity can be produced by gating them. One important gating mechanism is produced by intra- or extracellular ligands. Although the ligand-mediated channel gating is an important cellular process, the relationship between ligand binding and channel gating is not well understood. It is possible that ligands are involved in the interactions of different protein domains of the channel leading to opening or closing. To test this hypothesis, we studied the gating of Kir2.3 (HIR) by intracellular protons. Our results showed that hypercapnia or intracellular acidification strongly inhibited these channels. This effect relied on both the N and C termini. The CO(2)/pH sensitivities were abolished or compromised when one of the intracellular termini was replaced. Using purified N- and C-terminal peptides, we found that the N and C termini bound to each other in vitro. Although their binding was weak at pH 7.4, stronger binding was seen at pH 6.6. Two short sequences in the N and C termini were found to be critical for the N/C-terminal interaction. Interestingly, there was no titratable residue in these motifs. To identify the potential protonation sites, we systematically mutated most histidine residues in the intracellular N and C termini. We found that mutations of several histidine residues in the C but not the N terminus had a major effect on channel sensitivities to CO(2) and pH(i). These results suggest that at acidic pH, protons appear to interact with the C-terminal histidine residues and present the C terminus to the N terminus. Consequentially, these two intracellular termini bound to each other through two short motifs and closed the channel. Thus, a novel mechanism for K(+) channel gating is demonstrated, which involves the N- and C-terminal interaction with protons as the mediator. 相似文献
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'Working' cardiomyocytes exhibiting plateau action potentials from human placenta-derived extraembryonic mesodermal cells 总被引:3,自引:0,他引:3
Okamoto K Miyoshi S Toyoda M Hida N Ikegami Y Makino H Nishiyama N Tsuji H Cui CH Segawa K Uyama T Kami D Miyado K Asada H Matsumoto K Saito H Yoshimura Y Ogawa S Aeba R Yozu R Umezawa A 《Experimental cell research》2007,313(12):2550-2562
The clinical application of cell transplantation for severe heart failure is a promising strategy to improve impaired cardiac function. Recently, an array of cell types, including bone marrow cells, endothelial progenitors, mesenchymal stem cells, resident cardiac stem cells, and embryonic stem cells, have become important candidates for cell sources for cardiac repair. In the present study, we focused on the placenta as a cell source. Cells from the chorionic plate in the fetal portion of the human placenta were obtained after delivery by the primary culture method, and the cells generated in this study had the Y sex chromosome, indicating that the cells were derived from the fetus. The cells potentially expressed 'working' cardiomyocyte-specific genes such as cardiac myosin heavy chain 7beta, atrial myosin light chain, cardiac alpha-actin by gene chip analysis, and Csx/Nkx2.5, GATA4 by RT-PCR, cardiac troponin-I and connexin 43 by immunohistochemistry. These cells were able to differentiate into cardiomyocytes. Cardiac troponin-I and connexin 43 displayed a discontinuous pattern of localization at intercellular contact sites after cardiomyogenic differentiation, suggesting that the chorionic mesoderm contained a large number of cells with cardiomyogenic potential. The cells began spontaneously beating 3 days after co-cultivation with murine fetal cardiomyocytes and the frequency of beating cells reached a maximum on day 10. The contraction of the cardiomyocytes was rhythmical and synchronous, suggesting the presence of electrical communication between the cells. Placenta-derived human fetal cells may be useful for patients who cannot supply bone marrow cells but want to receive stem cell-based cardiac therapy. 相似文献
47.
通过野外观察并采用杂交指数(OCI)测定、花粉/胚珠比(P/O)检测、人工控制授粉等方法,对长萼兰花蕉(Orchidantha chinensis var.longisepala(D.Fang) T.L.Wu)种群的繁育系统进行了研究,采用常规石蜡切片与扫描电子显微镜(SEM)观察了柱头与"V"形黏盘的结构与形态。结果表明,长萼兰花蕉单花花期一般为18 d,依其花部形态的变化可分为蕾期、花萼未反转期、花萼反转期、唇瓣枯萎期、花萼枯萎期5个时期;根据杂交指数值为4、P/O值为253.89 ±21.09、人工异花授粉结实率分别为45%(2014年)和75%(2015年),显示出长萼兰花蕉的繁育系统属于异交,且需要传粉者。石蜡切片观察到长萼兰花蕉黏盘区与柱头可授区之间是光滑的表皮细胞,结合人工授粉实验与分泌物含糖量测定结果表明,长萼兰花蕉的"V"形黏盘不具有可授性,其作用可能是分泌黏液附着在传粉者背部使其便于携带花粉。长萼兰花蕉整个花期环境湿冷、多雨且开花同步性较低,这些因素很可能造成其有效传粉媒介缺乏,影响了传粉成功;另一方面,长萼兰花蕉有性繁殖受到限制,其主要通过根状茎进行无性繁殖后代,所以分布范围比较狭窄。 相似文献
48.
Junli Hu Xiangui Lin Junhua Wang Xiangchao Cui Jue Dai Haiyan Chu Jiabao Zhang 《Applied microbiology and biotechnology》2010,88(3):781-787
The P efficiency, crop yield, and response of wheat to arbuscular mycorrhizal fungus (AMF) Glomus caledonium were tested in an experimental field with long-term (19 years) fertilizer management. The experiment included five fertilizer
treatments: organic amendment (OA), half organic amendment plus half mineral fertilizer (1/2 OM), mineral fertilizer NPK,
mineral fertilizer NK, and the control (without fertilization). AMF inoculation responsiveness (MIR) of wheat plants at acquiring
P were estimated by comparing plants grown in unsterilized soil inoculated with G. caledonium and in untreated soil containing indigenous AMF. Without AMF inoculation, higher crop yields but lower colonization rates
were observed in the NPK and two OA-inputted treatments, and NPK had significantly (P < 0.05) lower impacts on organic C and available P in soils and thereby P acquisition of wheat plants compared with OA and
1/2 OM. G. caledonium inoculation significantly (P < 0.05) increased colonization rates with the NPK and two P-deficient treatments but significantly (P < 0.05) increased vegetative biomass, crop yield, and P acquisition of wheat as well as soil alkaline phosphatase (ALP) activity,
only with the NPK treatment. This gave an MIR of ca. 45% on total P acquisition of wheat plants. There were no other remarkable
MIRs. It suggested that the MIR is determined by soil available P status, and rational combination of AMF with chemical NPK
fertilizer can compensate for organic amendments by improving P-acquisition efficiency in arable soils. 相似文献
49.
Antitumor mechanism of Se-containing polysaccharide, a novel organic selenium compound 总被引:1,自引:0,他引:1
Dejing Shang Qiao Cui Yang Li Zhi Yu Lei Wen Yuan Zhao Jianing Zhang 《Frontiers of Biology in China》2009,4(3):248-253
Recent studies on the inhibition of tumor growth by Se-containing polysaccharide were reviewed. Meanwhile, the possible molecular
mechanisms of the inhibition of tumor cell growth through antioxidation, induction of tumor cell apoptosis, blockade of cell
cycle, and enhancement of immunity by Se-containing polysaccharide were proposed. In the end, the potential application of
Se-containing polysaccharide in the prevention and treatment of tumor was elucidated. 相似文献
50.
海洋芽孢杆菌(Bacillus marinus)B-9987菌株抑制病原真菌机理的研究 总被引:3,自引:0,他引:3
摘要:【目的】:探讨海洋芽孢杆菌(Bacillus marinus)B-9987菌株的代谢产物BMME-1,对植物病原真菌茄链格孢菌的抑菌作用机理。【方法】分别使用分光光法、气相色谱-质谱GC-MS联用技术、红外光谱法等,检测了BMME-1处理病原真菌后,菌体渗透性、细胞壁及细胞膜成份的变化。【结果】BMME-1对茄链格孢菌的抑菌中浓度(MIC50)为6.2 mg/L,最小杀菌浓度(MFC)为50 mg/L,在MIC50浓度或高于此浓度处理靶标菌,将导致菌体蛋白质、核酸等大分子物质的外流;处理菌株葡聚糖结 相似文献