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161.
Md Robiul Karim Sumei Zhang Fuchun Jian Jiacheng Li Chunxiang Zhou Longxian Zhang Mingfei Sun Guangyou Yang Fengcai Zou Haiju Dong Jian Li Farzana Islam Rume Meng Qi Rongjun Wang Changshen Ning Lihua Xiao 《International journal for parasitology》2014
Non-human primates (NHPs) are commonly infected with Cryptosporidium spp. and Giardia duodenalis. However, molecular characterisation of these pathogens from NHPs remains scarce. In this study, 2,660 specimens from 26 NHP species in China were examined and characterised by PCR amplification of 18S rRNA, 70 kDa heat shock protein (hsp70) and 60 kDa glycoprotein (gp60) gene loci for Cryptosporidium; and 1,386 of the specimens by ssrRNA, triosephosphate isomerase (tpi) and glutamate dehydrogenase (gdh) gene loci for Giardia. Cryptosporidium was detected in 0.7% (19/2660) specimens of four NHP species including rhesus macaques (0.7%), cynomolgus monkeys (1.0%), slow lorises (10.0%) and Francois’ leaf monkeys (6.7%), belonging to Cryptosporidium hominis (14/19) and Cryptosporidium muris (5/19). Two C. hominis gp60 subtypes, IbA12G3 and IiA17 were observed. Based on the tpi locus, G. duodenalis was identified in 2.2% (30/1,386) of specimens including 2.1% in rhesus macaques, 33.3% in Japanese macaques, 16.7% in Assam macaques, 0.7% in white-headed langurs, 1.6% in cynomolgus monkeys and 16.7% in olive baboons. Sequence analysis of the three targets indicated that all of the Giardia-positive specimens belonged to the zoonotic assemblage B. Highest sequence polymorphism was observed at the tpi locus, including 11 subtypes: three known and eight new ones. Phylogenetic analysis of the subtypes showed that most of them were close to the so-called subtype BIV. Intragenotypic variations at the gdh locus revealed six types of sequences (three known and three new), all of which belonged to so-called subtype BIV. Three specimens had co-infection with C. hominis (IbA12G3) and G. duodenalis (BIV). The presence of zoonotic genotypes and subtypes of Cryptosporidium spp. and G. duodenalis in NHPs suggests that these animals can potentially contribute to the transmission of human cryptosporidiosis and giardiasis. 相似文献
162.
163.
The importance of plant–soil feedback (PSF) has long been recognized, but the current knowledge on PSF patterns and the related mechanisms mainly stems from laboratory experiments. We aimed at addressing PSF effects on community performance and their determinants using an invasive forb Solidago canadensis. To do so, we surveyed 81 pairs of invaded versus uninvaded plots, collected soil samples from these pairwise plots, and performed an experiment with microcosm plant communities. The magnitudes of conditioning soil abiotic properties and soil biotic properties by S. canadensis were similar, but the direction was opposite; altered abiotic and biotic properties influenced the production of subsequent S. canadensis communities and its abundance similarly. These processes shaped neutral S. canadensis–soil feedback effects at the community level. Additionally, the relative dominance of S. canadensis increased with its ability of competitive suppression in the absence and presence of S. canadensis–soil feedbacks, and S. canadensis‐induced decreases in native plant species did not alter soil properties directly. These findings provide a basis for understanding PSF effects and the related mechanisms in the field conditions and also highlight the importance of considering PSFs holistically. 相似文献
164.
深远海浮游动物生态学研究进展 总被引:5,自引:0,他引:5
深远海浮游生态系统依据水深的差别可以被划分为几个子系统,包括上层、中层、深层和深渊层等。目前大量的研究结果表明在海洋上层,由于光照、温度、海冰和营养盐补充等因素的影响,浮游生物群落往往呈现出显著的时空变化。但在海洋中层及更深的区域,传统观点认为随着水深的增加,海洋的理化环境趋向于稳定,在这一区域生态系统的时空变化要弱于海洋上层。同时受调查技术和经费的限制,人们对于这一广阔区域内浮游生态系统时空变化规律的认识要局限的多。随着我国海洋科学的发展和海洋强国战略的实施,海洋科学研究也逐渐由过去的以近海研究为主发展到当前的近海、边缘海和深远海研究协同发展。但与我国近海浮游生态学过去数十年间积累的大量研究成果相比,我国科学家对深远海,特别是中层、深层和深渊层浮游生态学方面的研究极为缺乏。从大洋浮游动物群落的垂直分布及其变化、种间关系与生态位分化、深海浮游动物群落在碳沉降和生物地球化学循环中的作用等多个角度全面总结了当前国内外深海浮游生态学的研究进展,同时介绍过去十余年来深海浮游生态学研究技术手段上的巨大进步,以期为今后国内同行的研究提供参考和借鉴。 相似文献
165.
基于MapInfoProfessional 6 .0软件和野外调查所积累的资料 ,首先找出最佳“投资 效益比”的珍稀濒危物种密集分布区。随后 ,利用GAP分析的方法 ,对龙门河地区现状植被类型图、管理规划图、珍稀濒危物种密集分布区图进行了叠加分析 ,结果表明 :该地区规划管理的gap主要由 3部分组成 ,即原管理方法中不限制人为干扰的区域、原保护区域内没有包含的自然植被类型所属分布区、珍稀濒危物种密集分布区中没有禁止人为干扰的区域。其总面积为 1 736hm2 ,占龙门河地区总面积的 36 .81 %。综合考虑对植物多样性的保护及当地居民的生活所需 ,为该地区今后的管理重点提出了合理化建议 相似文献
166.
Cedric Hermans Ping Dong Marjorie Robin Michel Jadoul Alfred Bernard Andrew D. Bersten 《Biomarkers》2013,18(6):461-471
Increased leakage of surfactant proteins A and B (SP-A and SP-B) and Clara cell secretory protein (CC16) from the air spaces into the circulation occurs in a range of respiratory conditions. However, circulating levels depend not only on the rate of entry into the circulation, but also on the rate of clearance. In order to clarify the role of the kidney in the clearance of these proteins, serum levels were related to markers of glomerular filtration in 54 non-smoking patients with varying degrees of renal dysfunction, none of whom had respiratory disease or were receiving dialysis at the time of sampling. Serum SP-A was related to SP-B (r=0.53, p<0.001) and to CC16 (r=0.33, p<0.02). Similarly, SP-B was related to CC16 (r=0.39, p<0.004). Stepwise multiple linear regression analysis suggested that serum SP-A and SP-B are influenced by age (~20 and ~25% of variance, respectively), whereas CC16 is determined by renal function and, to a lesser extent, by body weight (~63% of variance in total). We conclude that CC16 is cleared from blood by the renal route, whereas SP-A and SP-B are not. Serum SP-A and SP-B are influenced by age, which we speculate reflects increased damage to the alveolocapillary barrier. 相似文献
167.
鲨鱼软骨血管生成抑制因子的纯化和功能 总被引:25,自引:0,他引:25
以中国东海鲸鲨软骨为原料,通过盐抽提、丙酮分级沉淀、离子交换层折、分子筛层析、高效液相色谱等步骤,获得鲨鱼软骨血管生成抑制因子-I(shark cartilgae angiogenesis inhibitory factor-I,SCAIF-I),对其分子量、抑制血管生成及抑制肿瘤生长活性进行了研究。结果显示SCAIF-I分子量18kD,在细胞和整体水平上显著抑制新血管生成,显著抑制小鼠肿瘤的生长 相似文献
168.
Xiaohan Sun Feng Wang Rong Cui Xiao Liu Xiangxiang Li Jibin Dong Lu Sun Siqi Qin Renqing Wang Peiming Zheng Hui Wang 《Ecology and evolution》2020,10(12):5270-5280
Vitex negundo L. var. heterophylla (Franch.) Rehder (Lamiaceae) is an important tree species for soil and water conservation, yet the reproductive ecology of this species remains to be elucidated. To investigate the reproductive traits of V. negundo var. heterophylla, the phenology, morphological characteristics (a suite of characters was assessed: floral morphology, nectar production, pollen viability, and stigma receptivity) and mating system of this species were systematically revealed for the first time in this study. Phenological observations, morphological measurements, and nectar production analysis were conducted during anthesis. Pollen viability and stigma receptivity at different flowering stages were measured by biochemical methods. Finally, genetic analysis based on SSR markers was used to reveal the mating system; outcrossing index and pollen‐ovule ratio were also calculated to help analysis. V. negundo var. heterophylla showed several obvious characteristics of outcrossing, such as abundant and attractive flowers, secreting nectar, and emitting scent. In addition, mechanisms such as homogamy and a short anther‐stigma distance that can promote self‐fertilization were also identified in this species. The coexistence of selfing and outcrossing characteristics demonstrates a predominantly outcrossed mixed mating system (outcrossing rate, t = 95%). The scientific information provided by this study may contribute to conservation of V. negundo var. heterophylla from a reproductive perspective. 相似文献
169.
随着全球塑料循环体系的变革升级,提高塑料的回收利用不仅可以减少塑料在生命周期中的碳排放,还可以解决废塑料潜在的生态环境危害。文中介绍了2019年国家自然科学基金组织间国际 (地区) 合作研究项目“废塑料资源高效生物降解转化的关键科学问题与技术 (MIXed plastics biodegradation and UPcycling using microbial communities,MIX-UP)”。该项目聚焦“塑料污染”这一全球化的问题,围绕中欧双方确定的“塑料生物降解菌群”研究领域,联合中欧双方14家优势科研单位,开展实质性的重大前沿合作研究。针对废塑料生物降解中存在的解聚与重塑两个难题,项目以难降解石油基塑料 (PP、PE、PUR、PET和PS) 以及生物可降解塑料 (PLA和PHA) 的混合废塑料作为研究对象,从塑料微生物降解途径解析及关键元件的挖掘与改造、塑料高效降解混菌/多酶体系的构建与功能调控、塑料降解物的高值化炼制途径设计与利用策略3个方面展开研究。本项目将突破废塑料生物降解转化中高效降解元件挖掘、塑料降解物高值化利用的关键科学问题与技术,探索一条废塑料资源化、高值化、循环化、低碳化的新塑料循环路线,建立以“降塑再造”为核心理念的废塑料生物炼制体系,丰富我国固废资源化生物技术利用平台。项目的实施不仅有助于提升我国塑料 (生物) 循环经济的理论基础和关键技术水平,还可以推动我国与国际科研院所的多边交流与合作,促进我国在生物技术领域的创新发展,助力我国碳中和目标的实现。 相似文献
170.
Naixiong Peng Zejian Zhang Yaomin Wang Minlong Yang Jiqing Fan Qinjun Wang Ling Deng Dong Chen Yuefeng Cai Qihui Li Xisheng Wang Wei Li 《Journal of cellular and molecular medicine》2021,25(22):10627-10637
Prostate cancer is the second most frequent malignancy in men worldwide, and its incidence is increasing. Therefore, it is urgently required to clarify the underlying mechanisms of prostate cancer. Although the long non-coding RNA LINC00115 was identified as an oncogene in several cancers, the expression and function of LINC00115 in prostate cancer have not been explored. Our results showed that LINC00115 was significantly up-regulated in prostate cancer tissues, which was significantly associated with a poor prognosis for prostate cancer patients. Functional studies showed that knockdown LINC00115 inhibited cell proliferation and invasion. In addition, LINC00115 served as a competing endogenous RNA (ceRNA) through sponging miR-212-5p to release Frizzled Family Receptor 5 (FZD5) expression. The expression of miR-212-5p was noticeably low in tumour tissues, and FZD5 expression level was down-regulated with the knockdown of LINC00115. Knockdown LINC00115 inhibited the Wnt/β‑catenin signalling pathway by inhibiting the expression of FZD5. Rescue experiments further showed that LINC00115 inhibits prostate cancer cell proliferation and invasion via targeting miR-212-5p/ FZD5/ Wnt/β-catenin axis. The present study provided clues that LINC00115 may be a promising novel therapeutic target for prostate cancer patients. 相似文献