湖南类石蛛科—新种(蛛形纲:蜘蛛目)

记述了中国湖南省类石蛛科垣蛛属一新种;大围垣蛛Ariadna daweiensis sp.nov。模式标本保存于湖南师范大学生命科学学院,文中量度单位为mm。     

重组人干扰素—β的中试研究

从大肠杆菌中大规模纯化新型干扰素β(IFN-βser17).首先建立IFN-βser17发酵和纯化工艺,连续大量纯化三批,并对纯化终产品进行全面鉴定.建立了稳定的发酵和纯化工艺流程,中试三批菌产量平均为4.33g/L,IFNβser17的表达量平均为20%,经破碎、粗提、精制后IFN-βser17的比活达2×107IU/mg以上,纯度超过96%,其他检定项目也均符合规程要求.     

气相色谱与触角电位检测器联用技术及其应用

介绍了气相色谱与触角电位检测器联用技术的工作原理、优点及其在昆虫性信息素或其它信息物质鉴定方面的应用。根据作者应用此技术进行赤松毛虫和桑尺蛾性信息素研究的经验 ,重点介绍了气相色谱与触角电位联用技术在实践操作中应注意的问题     

扬子地台西南缘早石炭世早期有孔虫化石带

扬子地台西南缘是早石炭世有孔虫动物群主要发育地区之一。本文通过滇东沾益、黔南独山、桂北罗城、环江、桂林、阳朔及灵川等10条剖面系统研究,首次从泥盆系顶部至维宪统底部建立了Quasiendothyra带,Bisphaera带,Chernyshinella带,Nodochernyshinella带(进而又分为N.tumulosa亚带和S pinotournayellina亚带）,Tuberendothyra带,Spinoendothyra带(分为Paradainella亚带和Psudodainella亚带Dainella带,Globoendothyra带,Palaeopaastaffella带及Eoparastaffella带等10个带和4个亚带,对每个带（及亚带）进行了有孔虫动物群详细分析。本文阐述了以N.tumulosa为代表的节结状次生沉积物有孔虫类出现,是早石炭早期有孔虫演化的一个重要阶段。通过Spinotournayellina,原始的tuberendothyrid,进化的tuberendothyrid至Spinoendothyra的出现。结合其它节结状和无节结状有孔虫层序特征详细分析了Tn2b-Tn3a间隔的有孔虫类一次次更新、演替关系,本文描述Spinotournayellina,Palaeospinoendothyra,Pseudoinflatoendothyra及Pseudodainella等4个新属及它们的模式种,简述了这些分类单位宏演化关系。对沿袭了半个世纪的岩关阶涵义作了修正,正限即为石炭系底界,始于Bisphaera带底界,上限止于Eoparastaffella带底界,并扼要地阐述了与西欧狄南盆地,北美同期地层对比关系。     

多效唑对花生叶片多胺含量及衰老的调节作用

通过对大田条件下生殖生长期花生喷施不同浓度的多效唑,研究了多效唑对叶片内源多受及其它衰老指标的影响,结果证明多效唑对内源多胺的影响较复杂,多效唑降低了幼嫩叶片的多胺含量,却提高了成熟叶片衰老阶段的多胺含量,并起到延缓衰老的作用,讨论了多效唑影响多胺含量的可能原因。     

山丹黄参的分布及微量元素含量研究

山丹黄参是伞形科迷果芹属的迷果芹（Sphallerocarpus gracilis)经测定,该植物含有大量人体必需的各种微量元素,其中P,Mg、Ｋ、Ｃu、Ｃr、Ｃo含量比党参（Codonopsis pilosula)和当归（Ａngelica sinensis)还高,Ｚn,Mn的水平也不低,是一种营养丰富的绿色食品,具有广泛的应用与开发前景。     

A simplified diphenylamine colorimetric method for growth quantification

Cell growth needs to be monitored in biological studies and bioprocess optimization. In special circumstances, such as microbial fermentations in media containing insoluble particles, accurate cell growth quantification is a challenge with current methods. Only the Burton method is applicable in such circumstances. The original Burton method was previously simplified by adopting a two-step sample pretreatment in perchloric acid procedure to eliminate the need for DNA extraction. Here, we further simplified the Burton method by replacing the previous two-step perchloric acid pretreatment with a new and one-step diphenylamine reagent pretreatment. The reliability and accuracy of this simplified method were assessed by measuring the biomass of four model microorganisms: Escherichia coli, Streptomyces clavuligerus, Saccharomyces cerevisiae, and Trichoderma reesei grown in normal media or those containing solid particles. The results demonstrate that this new simplified method performs comparably to the conventional methods, such as OD₆₀₀ or the previously modified Burton method, and is much more sensitive than the dry weight method. Overall, the new method is simple, reliable, easy to perform, and generally applicable in most circumstances, and it reduces the operation time from more than 12 h (for the previously simplified Burton method) to about 2 h.