全文获取类型
收费全文 | 76235篇 |
免费 | 5550篇 |
国内免费 | 4916篇 |
专业分类
86701篇 |
出版年
2024年 | 155篇 |
2023年 | 1033篇 |
2022年 | 2396篇 |
2021年 | 4074篇 |
2020年 | 2619篇 |
2019年 | 3236篇 |
2018年 | 3168篇 |
2017年 | 2298篇 |
2016年 | 3268篇 |
2015年 | 4815篇 |
2014年 | 5547篇 |
2013年 | 5989篇 |
2012年 | 7035篇 |
2011年 | 6162篇 |
2010年 | 3723篇 |
2009年 | 3342篇 |
2008年 | 3742篇 |
2007年 | 3372篇 |
2006年 | 2911篇 |
2005年 | 2382篇 |
2004年 | 1959篇 |
2003年 | 1654篇 |
2002年 | 1401篇 |
2001年 | 1230篇 |
2000年 | 1221篇 |
1999年 | 1122篇 |
1998年 | 661篇 |
1997年 | 655篇 |
1996年 | 667篇 |
1995年 | 617篇 |
1994年 | 544篇 |
1993年 | 377篇 |
1992年 | 568篇 |
1991年 | 435篇 |
1990年 | 406篇 |
1989年 | 282篇 |
1988年 | 244篇 |
1987年 | 234篇 |
1986年 | 166篇 |
1985年 | 194篇 |
1984年 | 109篇 |
1983年 | 117篇 |
1982年 | 71篇 |
1981年 | 58篇 |
1980年 | 37篇 |
1979年 | 61篇 |
1977年 | 30篇 |
1974年 | 38篇 |
1973年 | 34篇 |
1972年 | 30篇 |
排序方式: 共有10000条查询结果,搜索用时 0 毫秒
991.
992.
A Fully Verified Theoretical Analysis of Contact‐Mode Triboelectric Nanogenerators as a Wearable Power Source 下载免费PDF全文
Harvesting mechanical energy from human activities by triboelectric nanogenerators (TENGs) is an effective approach for sustainable, maintenance‐free, and green power source for wireless, portable, and wearable electronics. A theoretical model for contact‐mode triboelectric nanogenerators based on the principles of charge conservation and zero loop‐voltage is illustrated. Explicit expressions for the output current, voltage, and power are presented for the TENGs with an external load of resistance. Experimental verification is conducted by using a laboratory‐fabricated contact‐mode TENG made from conducting fabric electrodes and polydimethylsiloxane/graphene oxide composite as the dielectric layer. Excellent agreements of the output voltage, current, and power are demonstrated between the theoretical and experimental results, without any adjustable parameters. The effects of the moving speed on output voltage, current, and power are illustrated in three cases, that is, the motion with constant speed, the sinusoidal motion cycles, and the real walking cycles by human subject. The fully verified theoretical model is a very powerful tool to guide the design of the device structure and selection of materials, and optimization of performance with respect to the application conditions of TENGs. 相似文献
993.
The dynamics of a scroll wave in an excitable medium with gradient excitability is studied in detail. Three parameter regimes can be distinguished by the degree of gradient. For a small gradient, the system reaches a simple rotating synchronization. In this regime, the rigid rotating velocity of spiral waves is maximal in the layers with the highest filament twist. As the excitability gradient increases, the scroll wave evolutes into a meandering synchronous state. This transition is accompanied by a variation in twisting rate. Filament twisting may prevent the breakup of spiral waves in the bottom layers with a low excitability with which a spiral breaks in a 2D medium. When the gradient is large enough, the twisted filament breaks up, which results in a semi-turbulent state where the lower part is turbulent while the upper part contains a scroll wave with a low twisting filament. 相似文献
994.
995.
Production of chitinase from bacteria has distinct advantages over fungi, due to the formation of mycelia of fungi in the
later phase of fermentation. A novel chitinase-producing bacterial strain XJ-01 was isolated from the Yulu fishing field of
Changsha, Hunan province, China, by enrichment and spread-plate technique, sequentially. Physicochemical characterization
and 16S rRNA sequencing revealed that strain XJ-01 belongs to Serratia marcescens. By optimizing the fermentation condition based on L9(34) orthogonal experimental design, a maximal chitinase activity up to 15.36 U/ml was attained by that stain under the condition:
0.5% (NH4)2SO4 as the nitrogen source, 0.75% colloidal chitin as the carbon source, temperature of 32°C, time of 32 h and pH 8.0. 相似文献
996.
钛合金和钴铬合金表面白色念珠菌粘附的研究 总被引:3,自引:0,他引:3
目的比较钛合金(Ti-6Al-4V)和钴铬合金(Chromium-Cobaltalloy)表面白色念珠菌粘附能力的大小,研究表面粗糙度与细菌粘附的关系。方法将不同表面粗糙度的钛合金和钴铬合金试件进行白色念珠菌体外粘附试验,采用菌落形成计数法测定试件表面的细菌粘附量。结果各钛合金试件组的细菌粘附量均少于相同表面粗糙度的钴铬合金试件组,两种金属试件表面的细菌粘附量均随表面粗糙度的增大而增加。结论钛合金较钴铬合金更能减少由白色念珠菌引起的义齿性口炎等并发症,同时修复体表面严格的研磨抛光也能有效减少这些并发症。 相似文献
997.
Evidence for the presence of disulfide bridges in opioid receptors essential for ligand binding. Possible role in receptor activation 总被引:1,自引:0,他引:1
T L Gioannini Y F Liu Y H Park J M Hiller E J Simon 《Journal of molecular recognition : JMR》1989,2(1):44-48
The mobility of purified mu opioid binding protein in SDS-polyacrylamide gek electrophoresis is sensitive to the presence of reducing agents. In the presence of increasing concentrations of DTT the apparent molecular weight increases in a stepwise fashion from 53 kDa to 65 kDa. This reduction in mobility is attributed to the successive breakage of disulfide bridges, resulting in an increasingly asymmetric molecule. Treatment of cell membranes from various brain areas with reducing agents, such as DTT, produced a concentration-dependent inhibition of opioid binding. Sensitivity to DTT inhibition varied between receptor types, mu greater than delta much greater than kappa. For mu receptors, agonist binding was considerably more sensitive to DTT than antagonist binding. Inhibition by DTT is readily reversible and is unaffected by Na+ and/or Mg2+ ions. Reversibility may be partially prevented by the inclusion of a low concentration of a reducing reagent such as glutathione which does not inhibit binding but blocks reformation of disulfide bonds. Scatchard analysis of saturation data shows that DTT causes a pronounced decrease in binding affinity with little effect on receptor number. It is suggested that disulfide bonds are essential for ligand binding and that cleavage of one or more of these bonds may play a role in opioid receptor activation by agonists. 相似文献
998.
Nobuhiko Tada Shoji Kimura Yen Liu Benjamin A. Taylor Ulrich Hämmerling 《Immunogenetics》1981,13(6):539-546
Spleen cells from an SJL mouse immunized with 70'/3 cells, an established pre-B cell line, were fused with cells of the nonsecretor myeloma line NS.1. One established hybridoma cell line (clone K10.6) continuously secreted antibody that recognized a new antigenic specificity tentatively named Ly-m19. This newly found antigen is detectable on both T and B cells. Cytotoxicity assays reveal that 75 percent of the spleen and lymph-node cells, 35 percent of bone-marrow cells, and 15 percent of thymus cells reacted with antibody of clone K10.6. Strains expressing the specificity Ly-m19.1 are characterized by negative reactions and include the strains AKR, CE/J, RF/J, GR/A, SJL, P/J, BDP/J, and LG/J. All other strains so far tested are Ly-m19.2. This strain distribution pattern distinguishes Ly-m19 from any known murine lymphocyte alloantigen, but it parallels the Lyb-2
c
haplotype. Linkage test of a set of AKXL recombinant inbred strains revealed close linkage of Ly-m19 and Lyb-2 loci on mouse chromosome 4.Abbreviations used in this paper LPS
lipopolysaccharide
- B6
C57BL/6
- Con-A
concanavalin A
- MLC
mixed-lymphocyte culture
The prefix m (monoclonal) is used following a suggestion by Klein and co-workers (1979). 相似文献
999.
Rui Jia Qinghua Pan Shilei Ding Liwei Rong Shan-Lu Liu Yunqi Geng Wentao Qiao Chen Liang 《Journal of virology》2012,86(24):13697-13707
Interferon-inducible transmembrane (IFITM) protein family members IFITM1, -2, and -3 restrict the infection of multiple enveloped viruses. Significant enrichment of a minor IFITM3 allele was recently reported for patients who were hospitalized for seasonal and 2009 H1N1 pandemic flu. This IFITM3 allele lacks the region corresponding to the first amino-terminal 21 amino acids and is unable to inhibit influenza A virus. In this study, we found that deleting this 21-amino-acid region relocates IFITM3 from the endosomal compartments to the cell periphery. This finding likely underlies the lost inhibition of influenza A virus that completes its entry exclusively within endosomes at low pH. Yet, wild-type IFITM3 and the mutant with the 21-amino-acid deletion inhibit HIV-1 replication equally well. Given the pH-independent nature of HIV-1 entry, our results suggest that IFITM3 can inhibit viruses that enter cells via different routes and that its N-terminal region is specifically required for controlling pH-dependent viruses. 相似文献
1000.
抗原纯净度是口蹄疫(Foot-and-mouthdisease,FMD)灭活疫苗质量检验的一项重要内容,一般采用疫苗2–3次免疫动物后,检测非结构蛋白(Non-structuralprotein,NSP)抗体是否阳转,判断疫苗抗原的纯净度。文中旨在建立定量检测FMD灭活疫苗抗原中NSP3AB含量的ELISA方法,为疫苗质量控制提供参考方法。利用口蹄疫病毒(Foot-and-mouthdiseasevirus,FMDV)NSP3A单克隆抗体和辣根过氧化物酶(Horseradish peroxidase,HRP)标记的3B单克隆抗体,建立定量检测NSP3AB含量的双抗体夹心ELISA检测方法。采用原核表达并纯化的3AB蛋白作为标准品,标准品系列稀释,绘制标准曲线,以标准品与未加抗原的阴性对照吸光值(OD)的比值大于2.0的标准品最低浓度为最低检测限。标准品浓度介于4.7–600.0 ng/mL之间时,测得的OD值与浓度呈线性相关,回归曲线呈直线,相关系数R2=0.99,确定最低检测限为4.7ng/mL。检测12份未纯化灭活抗原中3AB蛋白含量介于9.3–200.0ng/mL之间;而纯化后的... 相似文献