全文获取类型
收费全文 | 31971篇 |
免费 | 2769篇 |
国内免费 | 3754篇 |
出版年
2024年 | 71篇 |
2023年 | 510篇 |
2022年 | 1030篇 |
2021年 | 1735篇 |
2020年 | 1150篇 |
2019年 | 1574篇 |
2018年 | 1407篇 |
2017年 | 1060篇 |
2016年 | 1496篇 |
2015年 | 2024篇 |
2014年 | 2513篇 |
2013年 | 2595篇 |
2012年 | 3091篇 |
2011年 | 2765篇 |
2010年 | 1770篇 |
2009年 | 1562篇 |
2008年 | 1765篇 |
2007年 | 1537篇 |
2006年 | 1298篇 |
2005年 | 1128篇 |
2004年 | 960篇 |
2003年 | 839篇 |
2002年 | 745篇 |
2001年 | 513篇 |
2000年 | 472篇 |
1999年 | 462篇 |
1998年 | 311篇 |
1997年 | 296篇 |
1996年 | 261篇 |
1995年 | 194篇 |
1994年 | 189篇 |
1993年 | 129篇 |
1992年 | 188篇 |
1991年 | 157篇 |
1990年 | 152篇 |
1989年 | 110篇 |
1988年 | 71篇 |
1987年 | 48篇 |
1986年 | 57篇 |
1985年 | 56篇 |
1984年 | 34篇 |
1983年 | 41篇 |
1982年 | 24篇 |
1981年 | 20篇 |
1980年 | 10篇 |
1979年 | 21篇 |
1978年 | 6篇 |
1977年 | 10篇 |
1976年 | 9篇 |
1975年 | 7篇 |
排序方式: 共有10000条查询结果,搜索用时 218 毫秒
31.
Yuhui He Makusu Tsutsui Ralph H. Scheicher Chun Fan Masateru Taniguchi Tomoji Kawai 《Biophysical journal》2013
Experiments using nanopores demonstrated that a salt gradient enhances the capture rate of DNA and reduces its translocation speed. These two effects can help to enable electrical DNA sequencing with nanopores. Here, we provide a quantitative theoretical evaluation that shows the positive net charges, which accumulate around the pore entrance due to the salt gradient, are responsible for the two observed effects: they reinforce the electric capture field, resulting in promoted molecule capture rate; and they induce cationic electroosmotic flow through the nanopore, thus significantly retarding the motion of the anionic DNA through the nanopore. Our multiphysical simulation results show that, during the polymer trapping stage, the former effect plays the major role, thus resulting in promoted DNA capture rate, while during the nanopore-penetrating stage the latter effect dominates and consequently reduces the DNA translocation speed significantly. Quantitative agreement with experimental results has been reached by further taking nanopore wall surface charges into account. 相似文献
32.
Yan-Hong He Guo-Gui Ning Ya-Lin Sun Yan Hu Xing-Yu Zhao Man-Zhu Bao 《Molecular breeding : new strategies in plant improvement》2010,26(1):19-29
A male sterile line was isolated in marigold (Tagetes erecta L.) and cytological analysis determined this to be a novel genic male sterility trait (Tems). Through the use of amplified fragment length polymorphisms (AFLPs) and bulked segregant analysis (BSA), tightly linked
markers of Tems were identified with a view towards a map-based cloning strategy. It was found that spontaneous homeotic conversion of floral
organs was the underlying cause of the male sterility in this marigold line. Thus, petals of male sterile plants resembled
sepal-like structures and the stamens were partially converted to styles, although without the full characteristics or function
of the true style organs. We have constructed a fine marker-based map for the Tems gene. This is intended to provide a tool for marker assisted selection (MAS) strategies in hybrid breeding and map-based
cloning strategies for the male sterility locus. We discuss the significance of this spontaneously derived genic male sterility
trait relating to the homeotic conversion of floral organs in marigold. 相似文献
33.
34.
我国某些常见化石硅藻的环境分析 总被引:6,自引:0,他引:6
蒋辉 《Acta Botanica Sinica》1987,(4)
本文详细研究了中国海表层沉积物中的常见化石硅藻,结果表明,这些种的分布范围及数量变化随环境的不同而变化。因此,根据柱状样品中这些种类数量的变化,能够推断古气候变化,恢复古地理环境,再现海平面波动历程,进而划分、对比第四系地层。 相似文献
35.
Harringtonine showed cross resistance in adriamycin-resistant murine leukemia P388 (P388/ADM) and human leukemia K562 (K562/ADM) cells. The relative resistance of the P388/ADM and K562/ADM cells to harringtonine was about 7 and 40, respectively. Calcium influx blockers, diltiazem and the biscoclaurine alkaloid cepharanthine enhanced the cytotoxicity of harringtonine in P388/ADM and K562/ADM cells. The extent of enhancement was different for the two drugs, and up to a 9- to 10-fold increase in harringtonine cytotoxicity occurred in P388/ADM cells, and 14- to 22-fold enhancement in K562/ADM cells with diltiazem or cepharanthine. Harringtonine resistance of P388/ADM was circumvented completely, and the resistance of K562/ADM was circumvented partially, by diltiazem or cepharanthine. The mechanism of enhanced cytotoxicity by diltiazem and cepharanthine is probably inhibition of active efflux of harringtonine in P388/ADM and K562/ADM cells. 相似文献
36.
Scavenging effect of extracts of green tea and natural antioxidants on active oxygen radicals 总被引:14,自引:0,他引:14
With the use of the spin trapping methods, the scavenging effects of the extracts of green tea and other natural foods are studied. In stimulated polymorphonuclear leukocytes (PMN) system, water extract fraction 6 (F6) from green tea and green tea polyphenols (GTP) have the strongest scavenging effect on the active oxygen radicals, much stronger than vitamin C (Vc) and vitamin E (VE). Rosemary antioxidants (RA) and Curcumin (Cur) have weaker scavenging effects than Vc, but stronger than VE. In Fenton Reaction, Cur has the strongest scavenging effect (69%) on hydroxyl radicals. In irradiation, riboflavin system F6(74%) and GTP(72%) have very strong scavenging effects that are weaker than Vc, but much stronger than VE (23%). With the use of spin probe oxymetry, the oxygen consumption in respiratory burst of stimulated PMN were measured when the antioxidants existed in these systems. The results demonstrated that these antioxidants did not affect the respiratory burst of human polymorphonuclear leukocytes stimulated with PMA. 相似文献
37.
延胡索分类的化学证据 总被引:2,自引:0,他引:2
东阳产延胡索与大连产齿瓣延胡索经成分分离和TLC、HPLC对比,发现延胡索以啊扑啡类生物碱如glaucine为主,而齿瓣延胡索则含corynoline类生物碱。根据生物碱的类型及含量比较,二者有明显差异,结合延胡索的植物形态和植化分类特征判断,将延胡索作为与齿瓣延胡索近缘的独立种处理较为合理,即为Corydalis yanhusuo W. T. Wang ex Z.Y. Su et C. Y. Wu 相似文献
38.
大壁虎的染色体及减数分裂联会复合体的研究 总被引:7,自引:1,他引:6
大壁虎(Gekko gecko)的染色体数目为2n=38,核型由2对中着丝粒(Nos.1.4.)、3对亚中着丝粒(Nos.2.3.5)及14对端着丝粒和亚端着丝粒(Nos.6—19)染色体组成。一对核仁组织者(NOR_s),位于第7对端着丝粒染色体的末端。同时,本文还对大壁虎的减数分裂以及联会复合体(S.C)的结构和组型,进行了详细的观察和分析。 相似文献
39.
Modelling basic features of specificity in the binding of a dicationic steroid diamine to double-stranded oligonucleotides. 下载免费PDF全文
An investigation of the intrinsically preferred binding modes of a steroid diamine, dipyrandium, to the double-stranded hexanucleotides d(TATATA)2, d(ATATAT)2, and d(CGCGCG)2 is carried out by the energy minimization procedure JUMNA. Several alternative binding modes are compared: groove binding in which the conformation of the oligonucleotide remains close to that of B-DNA, intercalation between base-pairs and interaction with variously kinked structures in which base pairs of dinucleoside steps open towards the groove in which the binding occurs. The favored binding configuration occurs at the d(TpA) step of the AT kinked nucleotides in which the kink opens the base pairs towards the minor groove. Thus, for the d(T1A2T3A4T5A6)2 sequences the preferred complexation involves the kink at the T3A4 step facing the cyclohexane rings A, B, and C of the ligand. For the d(A1T2A3T4A5T6)2 sequence, the kink occurs at the T2A3 step facing the cationic pyrrolidine ring linked to ring A. The binding of dipyrandium to d(CGCGCG)2 is found to be considerably less favourable than for either of the two (AT) sequences. 相似文献
40.