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Objectives: Technical limitations and evolution of therapeutic applications for cell culture-derived products have accelerated elimination of animal-derived constituents from such products to minimize inadvertent introduction of microbial contaminants, such as fungi, bacteria or viruses. The study described here was conducted to investigate the proliferative effect of vegetable peptone on adult stem cells in the absence of serum, and its possible mechanisms of action.
Materials and methods: Cell viability and proliferation were determined using the MTT assay and Click-iT™ EdU flow cytometry, respectively. In addition, changes in expression of cytokine genes were analysed using MILLIPLEX™ human cytokine enzyme-linked immunosorbent assay kit.
Results: Viability of cord blood-derived mesenchymal stem cells (CB-MSC) and adipose tissue-derived stem cells (ADSC) increased significantly when treated with the peptone. In addition, median value of the group treated with peptone shifted to the right when compared to the untreated control group. Furthermore, quantitative analysis of the cytokines revealed that production of vascular endothelial growth factor (VEGF), transforming growth factor-beta1 (TGF-β1), and interleukin-6 (IL-6) increased significantly in response to treatment with our vegetable peptone in both CB-MSCs and ADSCs.
Conclusions: Our findings revealed that the vegetable peptone promotes proliferation of CB-MSCs and ADSCs. In addition, results of this study suggest that induction of stem cell proliferation by vegetable peptone is likely to be related to its induction of VEGF, TGF-β1, and IL-6 expression. 相似文献
Materials and methods: Cell viability and proliferation were determined using the MTT assay and Click-iT™ EdU flow cytometry, respectively. In addition, changes in expression of cytokine genes were analysed using MILLIPLEX™ human cytokine enzyme-linked immunosorbent assay kit.
Results: Viability of cord blood-derived mesenchymal stem cells (CB-MSC) and adipose tissue-derived stem cells (ADSC) increased significantly when treated with the peptone. In addition, median value of the group treated with peptone shifted to the right when compared to the untreated control group. Furthermore, quantitative analysis of the cytokines revealed that production of vascular endothelial growth factor (VEGF), transforming growth factor-beta1 (TGF-β1), and interleukin-6 (IL-6) increased significantly in response to treatment with our vegetable peptone in both CB-MSCs and ADSCs.
Conclusions: Our findings revealed that the vegetable peptone promotes proliferation of CB-MSCs and ADSCs. In addition, results of this study suggest that induction of stem cell proliferation by vegetable peptone is likely to be related to its induction of VEGF, TGF-β1, and IL-6 expression. 相似文献
713.
Ho Yul Choo Harry K. Kaya Jin Huh Dong Woon Lee Hyeong Hwan Kim Sang Myeong Lee Young Moo Choo 《BioControl》2002,47(2):177-192
Three species of entomopathogenicnematodes, a combination of two nematodespecies, an entomopathogenic fungal species,and a combination of a nematode and fungalspecies were evaluated against the white grubsEctinohoplia rufipes and Exomalaorientalis (Coleoptera: Scarabaeidae) in the field. The nematodes were acommercial formulation of Steinernemacarpocapsae (BioSafe) and S. glaseri from Dongrae and from Hanrim, and Heterorhabditis bacteriophora from Hamyang,Republic of Korea. The entomopathogenic funguswas Beauveria brongniartii, produced onSabouraud maltose agar plus 1% yeast (SMAY),rice bran, or compost. The combinationtreatment was S. carpocapsae with H.bacteriophora or B. brongniartii. Fieldapplications were made in August or Septemberagainst third instars at a golf course infestedwith E. rufipes in Gyeongnam Province in1991, and one in Pusan with E. orientalisin 1992 and 1993. In 1991, a significantreduction of 70.2 to 79.4% of E. rufipeslarvae was observed in the nematode, fungal andchemical (fenitrothion) treatments comparedwith a 15.7% reduction in the control. In1992, the E. orientalis larval populationwas reduced between 62.7 and 82.8% in thetreatments compared to 10.7% in the control.In 1993, larval reductions in plots treatedwith nematodes (78.3 to 97%) and B.brongniartii propagated on rice bran (84.5%)were significantly better than in plots treatedwith B. brongniartii propagated on SMAY(63.6%) or compost (59.6%). Combining twonematode species did not enhance the efficacycompared to treatments with one nematodespecies alone, but combining S.carpocapsae with B. brongniartiiproduced on SMAY resulted in a significantincrease in grub mortality over the applicationof the fungus alone produced on SMAY orcompost. The high efficacy of the nematode andmost fungal treatments was attributed to theclose proximity of the white grubs to the soilsurface which allowed for excellentpathogen-host contact and to favorable soiltemperatures, sandy soil, post irrigationapplication and/or rain and a minimal thatchlayer in the turfgrass. 相似文献
714.
S H Lee J Y Chai S T Hong W M Sohn S Huh E H Cheong S B Kang 《The Korean journal of parasitology》1989,27(3):213-216
Seven cases of Diphyllobothrium latum infection were proved by collection of worms after praziquantel treatment between October, 1986 and July, 1987. The patients were all males aged 20-44 years residing in Seoul or Ulungdo, Kyungpook Province. All of them had the history of eating several kinds of raw marine fishes, and they had never been to abroad. One of them experienced abdominal pain and 6 experienced natural discharge of a chain of worm segments, but none revealed any sign of anemia. Total 12 worms (1-3/patient) were collected after praziquantel treatment. The worms were 85-423 cm in length, and revealed the characteristic rosette-shape uterus in their gravid proglottides. The average egg size varied 61.0-65.3 x 41.7-46.1 microns. The eggs were yellowish-brown, and ovoid to elliptical. Including the present 7 cases, the total number of human D. latum infections proven by worms in Korea becomes 28 cases. 相似文献
715.
Sang Hoon Paik Yong Jae Kim Sang Kyul Han Jean Man Kim Jae Wook Huh Young In Park 《Biotechnology progress》2012,28(6):1517-1525
A formulation with stabilizers replacing albumin was developed for lyophilization of recombinant factor VIII (FVIII), GreenGene F (WHO INN: beroctocog alfa), to achieve stability and eliminate safety issues of blood‐derived albumin. L ‐Arginine (hydrophilic amino acid, positively charged side chain), L ‐glutamic acid (hydrophilic amino acid, negatively charged side chain), and L ‐isoleucine (hydrophobic amino acid, nonpolar) were selected as stabilizers, and the mixture of the three amino acids were optimized. The mixture had results comparative with albumin and other commonly used stabilizers showing good preservation of recombinant FVIII during lyophilization, robust stability with consistently high recovery of FVIIII, very low aggregate formation, and good storage stability without alterations in protein characteristics. In vivo test results showed that the efficacy was maintained and had no signs of toxicity. The study demonstrated that the three amino acid mixture acts as a good stabilizer for lyophilization of recombinant FVIII and as a safe excipient. © 2012 American Institute of Chemical Engineers Biotechnol. Prog., 2012 相似文献
716.