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141.
Anne Gyiochon-Mantel Hugues Loosfelt Pierre Lescop Sophie Christin-Maitre Martine Perrot-Applanat Edwin Milgrom 《The Journal of steroid biochemistry and molecular biology》1992,41(3-8):209-215
Deletion mutants of the rabbit progesterone receptor were used to identify two major mechanisms of its nuclear localization. A putative signal sequence, homologous to that of the SV40 large T antigen, was localized around amino acids 638–642 and was shown to be constitutively active. When amino acids 638–642 were deleted, the receptor became cytoplasmic but could be shifted into the nucleus by the addition of hormone (or antihormone), it was almost fully active. A second putative nuclear localization signal is located in the DNA binding domain activated either through ligand binding or through production of constitutive receptor. By deleting epitopes recognized by monoclonal antibodies, it was possible to follow different receptor mutants inside the same cells. In the absence of ligand the receptor was transferred into the nucleus as a monomer. After administration of hormone (or antihormone) a “cytoplasmic” monomer was transferred into the nucleus through interaction with a “nuclear” monomer. These interactions occurred through the steroid binding domains of both monomers. 相似文献
142.
Mathieu Gautier Junichi Yamaguchi Julien Foucaud Anne Loiseau Aurélien Ausset Benoit Facon Bernhard Gschloessl Jacques Lagnel Etienne Loire Hugues Parrinello Dany Severac Celine Lopez-Roques Cecile Donnadieu Maxime Manno Helene Berges Karim Gharbi Lori Lawson-Handley Lian-Sheng Zang Benjamin Prud’homme 《Current biology : CB》2018,28(20):3296-3302.e7
143.
Chantal Andre Marie-Claude Guillemin Jun Zhu Marcel H. M. Koken Frdrique Quignon Laurence Herve Mounira K. Chelbi-Alix Daniel Dhumeaux Zeng-Yi Wang Laurent Degos Zhu Chen Hugues de The 《Experimental cell research》1996,229(2):253
Acute promyelocytic leukemia (APL) is specifically associated to a t(15; 17) translocation which fuses a gene encoding a nuclear receptor for retinoic acid, RARα, to a previously unknown gene PML. The PML protein is localized in the nucleus on a specific domain of unknown function (PML nuclear bodies, NB) previously detected with autoimmune sera from patients with primary biliary cirrhosis (PBC). These bodies are nuclear matrix-associated and all of their identified components (PML, Sp100, and NDP52) are sharply upregulated by interferons. We show that autoantibodies against both PML and Sp100 are usually associated in sera with multiple nuclear dot anti-nuclear antibodies and demonstrate that PML is an autoantigen, not only in PBC, but also in other autoimmune diseases. In APL, the PML/RARα fusion interferes with both the retinoic acid (RA) response and PML localization on nuclear bodies, but the respective contribution of each defect to leukemogenesis is unclear. RA induces the terminal differentiation of APL blasts, yielding to complete remissions, and corrects the localization of NB antigens. Arsenic trioxide (As2O3) also induces remissions in APL, seemingly through induction of apoptosis. We show that in APL, As2O3leads to the rapid reformation of PML bodies. Thus, both agents correct the defect in NB antigen localization, stressing the role of nuclear bodies in the pathogenesis of APL. 相似文献
144.
Variability in storage potential of banana shoot cultures under medium term storage conditions 总被引:3,自引:0,他引:3
Ines Van den houwe Kris De Smet Hugues Tezenas du Montcel Rony Swennen 《Plant Cell, Tissue and Organ Culture》1995,42(3):269-274
Shoot cultures of 401 banana clones were conserved under slow growth conditions (16±1°C, 25mol m–2 s–1). Storage duration-defined as 60% survival time of 20 shoot cultures of a clone-averaged 334 days. However, large differences occurred among the different genomic (sub)groups and even within the same (sub)group. East-African highland bananas and non-plantain AAB bananas can be stored for significantly longer periods. Shoot tip cultures of another 41 banana clones conserved at higher ambient temperature (22±3°C) needed to be subcultured sooner (every 220 days on average).Abbreviations BA
6-benzyladenine
- CIRAD
Centre de Coopération Internationale en Recherche Agronomique pour le Développement
- IAA
indole-3-acetic acid
- IBPGR
International Board for Plant Genetic Resources
- INIBAP
International Network for the Improvement of Banana and Plantain
- PPF
photosynthetic photon flux
- QDPI
Queensland Department of Primary Industries 相似文献
145.
Nicolas Latte François Lebourgeois Hugues Claessens 《Trees - Structure and Function》2016,30(1):189-201
Key message
Beech growth variability and climate sensitivity are much higher in the crown top than in the bole. The most notable bole–crown discrepancies occurred in response to extreme climate conditions.Abstract
To characterize growth partitioning within the tree and its responses to climate, we studied eight dominant beech trees (Fagus sylvatica L.) of a pure, even-aged 98-year-old stand in Belgium. We sampled ten disks along the stem from breast height to treetop and examined the inter-annual patterns of, and discrepancies between, ring-area and volume increments by performing detailed stem analysis and dendroecological investigations. Although the common inter-annual variation among all increment series was high, we observed increasing growth variability and climate sensitivity with height, leading to notable bole–crown discrepancies. Both the common inter-annual variation and bole–crown discrepancies were mainly driven by summer heat waves and related droughts of the previous year, and spring droughts of the current year. Despite these discrepancies, the radial growth at breast height can be considered a good estimate of the tree volume increment but not for the purpose of focusing on climatic effects of isolated years. Extreme climatic conditions increase the risk of inaccurate estimations. The results of the present study are discussed in relation to tree ecophysiology hypotheses.146.
Sarah Parisot Amélie Darlix Cédric Baumann Sonia Zouaoui Yordanka Yordanova Marie Blonski Valérie Rigau Stéphane Chemouny Luc Taillandier Luc Bauchet Hugues Duffau Nikos Paragios 《PloS one》2016,11(1)
Diffuse WHO grade II gliomas are diffusively infiltrative brain tumors characterized by an unavoidable anaplastic transformation. Their management is strongly dependent on their location in the brain due to interactions with functional regions and potential differences in molecular biology. In this paper, we present the construction of a probabilistic atlas mapping the preferential locations of diffuse WHO grade II gliomas in the brain. This is carried out through a sparse graph whose nodes correspond to clusters of tumors clustered together based on their spatial proximity. The interest of such an atlas is illustrated via two applications. The first one correlates tumor location with the patient’s age via a statistical analysis, highlighting the interest of the atlas for studying the origins and behavior of the tumors. The second exploits the fact that the tumors have preferential locations for automatic segmentation. Through a coupled decomposed Markov Random Field model, the atlas guides the segmentation process, and characterizes which preferential location the tumor belongs to and consequently which behavior it could be associated to. Leave-one-out cross validation experiments on a large database highlight the robustness of the graph, and yield promising segmentation results. 相似文献
147.
148.
B Brun C Cranz B Ishak A Clavert F Hugues M Leclerc Y Rumpler 《Folia primatologica; international journal of primatology》1987,48(3-4):195-198
A method is reported for collection of sperm by electrostimulation or prostatic massage in order to carry out artificial in utero insemination in Lemur fulvus mayottensis. Using fresh sperm, one success out of 7 attempts was obtained, although it was achieved with a vesiculectomized male. 相似文献
149.
Chemical modifications of scyllatoxin (leiurustoxin I) have shown that two arginines in the sequence, Arg6 and Arg13, are essential both for binding to the Ca(2+)-activated K+ channel protein and for the functional effect of the toxin. His31 is important both for the binding activity of the toxin and for the induction of contractions on taenia coli. However, although its iodination drastically decreases the toxin activity, it does not abolish it. Chemical modification of lysine residues or of Glu27 does not significantly alter toxin binding, but it drastically decreases potency with respect to contraction of taenia coli. The same observation has been made after chemical modification of the lysine residues. The brain distribution of scyllatoxin binding sites has been analyzed by quantitative autoradiographic analysis. It indicates that apamin (a bee venom toxin) binding sites are colocalized with scyllatoxin binding sites. The results are consonant with the presence of apamin/scyllatoxin binding sites associated with Ca(2+)-activated K+ channels. High-affinity binding sites for apamin can be associated with very-high-affinity (less than 70 pM), high-affinity (approximately 100-500 pM), or moderate-affinity (greater than 800 pM) binding sites for scyllatoxin. 相似文献
150.
Pityopus californicus (Eastw.) H. F. Copel., a monotypic member of the Monotropoideae in the family Ericaceae, is a myco-heterotrophic species
with distribution limited to the Pacific Northwest of the USA. Young embryos of P. californicus developed mycorrhizal associations in seed packets that had been buried for up to 681 days, suggesting that seeds of P. californicus may require the presence of a fungus to achieve germination. Samples of nongerminated seeds and early stages in embryo and
root development were subsequently processed for light microscopy, histochemistry, and transmission electron microscopy (TEM).
Nongerminated seeds possessed a thick testa, lacked a shoot and root meristem, and consisted of an embryo with large parenchymatous
cells containing protein bodies and starch grains as storage reserves. In the earliest developmental stage (seed coat still
attached), fungal hyphae were present on the testa surface and between the testa and embryo. This stage was followed by embryo
elongation, the organization of a root apical meristem, and the development of a well-developed fungal mantle surrounding
the elongated embryo. At least two morphotypes were identified based on structural characteristics of the mantle. One of these,
with ascomycetous septa, had Cenococcum-like features. Late-stage embryo/early root development revealed a typical mantle and Hartig net, with fungal pegs penetrating
the outer tangential walls of epidermal cells. Transfer cell-like deposits of wall material, similar to those described in
Monotropa spp., enclosed fungal pegs. The development of a Hartig net and fungal pegs suggests that nutrient exchange interfaces are
required for seedling development. 相似文献