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Y.Q. Shirleen Soh Jessica Alföldi Tatyana Pyntikova Laura G. Brown Tina Graves Patrick J. Minx Robert S. Fulton Colin Kremitzki Natalia Koutseva Jacob L. Mueller Steve Rozen Jennifer F. Hughes Elaine Owens James E. Womack William J. Murphy Qing Cao Pieter de Jong Wesley C. Warren Richard K. Wilson Helen Skaletsky David C. Page 《Cell》2014
995.
Eric A. Balcueva Qin Wang Heather Hughes Charles Kunsch Zihui Yu Janet D. Robishaw 《Experimental cell research》2000,257(2):310
The mammalian γ subunit family consists of a minimum of 12 members. Analysis of the amino acid sequence conservation suggests that the γ subunit family can be divided into three distinct subclasses. The division of the γ subunit family into these classes is based not only on amino acid homology, but also to some extent on functional similarities. In the present study, two new members of the γ subunit family, the γ11 and γ14 subunits, are identified and characterized in terms of their expression and function. The γ11 and γ14 subunits are most closely related to the γ1 subunit and share similar biochemical properties, suggesting their inclusion in class I. However, despite their close phylogenetic relationship and similar biochemical properties, the γ1, γ11, and γ14 subunits exhibit very distinct expression patterns, suggesting that class I should be further subdivided and that the signaling functions of each subgroup are distinct. In this regard, the γ11 and γ14 subunits represent a new subgroup of farnesylated γ subunits that are expressed outside the retina and have functions other than phototransduction. 相似文献
996.
Incubation of porcine leukocytes with [1-14C]-15-hydroperoxyeicosapentaenoic acid (15-HPEPE) results in the formation of a group of polar metabolites which after separation and purification by RP-HPLC and SP-HPLC were found to be a series of new compounds containing three hydroxy groups and four conjugated double bonds. The structures of these new metabolites were established by U. V. spectrophotometry and GC/MS to be trihydroxy pentaenes of EPA, i.e., 5,6,15-trihydroxy-7,9,11,13,17-eicosapentaenoic acid and 5,14,15-trihydroxy-6,8,10,12,17-eicosapentaenoic acid. Because of the additional double bond at C17-18, these two new metabolites of EPA were proposed to be lipoxene A and lipoxene B, respectively. 相似文献
997.
P F Daels S Shideler B L Lasley J P Hughes G H Stabenfeldt 《Journal of reproduction and fertility》1990,90(1):55-61
Oestrogen secretion was determined by oestrogen conjugate (EC) analysis of urine in three groups of pregnant mares: Group I (N = 6), animals ovariectomized on Day 18-19 of gestation with pregnancy maintained by daily administration of an oral progestagen, altrenogest; Group II (N = 9), untreated, pregnant mares; Group III (N = 5) intact, pregnant mares treated daily with altrenogest. The mean EC concentrations in the ovariectomized mares in Group I increased in a constant linear manner from 17 ng/mg Cr on Day 20 to 291 ng/mg Cr on Day 70, with no apparent surge in oestrogen secretion around Day 39. Mean EC concentrations on Days 33, 39 and 44 were respectively 41, 48, and 73 ng/mg Cr. In the intact mares in Groups II and III (shown in parentheses), the mean urinary EC concentrations were 201 (171) ng/mg Cr between Days 20 and 33 of gestation, increased rapidly from 172 (77) ng/mg Cr on Day 33 to a peak of 1066 (895) ng/mg Cr on Day 39, followed by a decline to 637 (719) ng/mg Cr on Day 44. After Day 44, EC concentrations continued to increase in a linear manner to 1191 (842) ng/mg Cr on Day 70. The mean EC concentrations between Days 20 and 70 in Group I were significantly (P less than 0.05) lower than in mares in Groups II and III. EC concentrations in Group III mares were significantly lower (P less than 0.05) than in Group II mares between Days 28 and 34.(ABSTRACT TRUNCATED AT 250 WORDS) 相似文献
998.
Recently, it has been reported that impotence in the stallion has a physiological basis that involves decreased serum concentrations of luteinizing hormone (LH) and estradiol-17beta, but not testosterone. We have found such a hormonal profile in two of nine stallions studied during an ongoing investigation of the endocrinology of the normal stallion. Nevertheless, both of these stallions possessed vigorous libido and normal seminal characteristics. We conclude that the hormonal profile of low LH, low estradiol and normal testosterone, although it may accompany impotence in the stallion, is not predictive of, or causally related to, abnormalities in sexual behavior. 相似文献
999.
India ink was found to be an acceptable stain for proteins blotted or dotted onto positively charged nylon or hydrophobic membranes. The hydrophobic membrane, Immobilon, was an outstanding matrix for binding proteins and displayed low levels of background staining. The least amount of protein detected by india ink staining was between 1.0 and 10 ng. India ink staining of proteins on nylon membranes is an easy, inexpensive, and quick method for the unequivocal detection of both standards and unknowns in the same blot. However, inks, ink concentrations, fixing conditions, staining times, pH, washing conditions, and membrane lots all need to be controlled to achieve maximum sensitivity for protein detection following india ink staining. 相似文献
1000.
Coaggregation properties of human oral Veillonella spp.: relationship to colonization site and oral ecology. 总被引:6,自引:1,他引:5 下载免费PDF全文
The primary habitats of oral veillonellae are the tongue, dental plaque, and the buccal mucosa. Isolates were obtained from each habitat and tested for coaggregation with a battery of other oral bacterial strains. All 59 tongue isolates tested for coaggregation were Veillonella atypica or Veillonella dispar. All but one of them coaggregated with strains of Streptococcus salivarius, a predominant inhabitant of the tongue surface but not subgingival dental plaque. These tongue isolates were unable to coaggregate with most normal members of the subgingival flora such as Actinomyces viscosus, Actinomyces naeslundii, Actinomyces israelii, and Streptococcus sanguis. In contrast, 24 of 29 Veillonella isolates, of which 20 were Veillonella parvula from subgingival dental plaque samples, coaggregated strongly with the three species of Actinomyces, S. sanguis, and other bacteria usually present in subgingival plaque, but they did not coaggregate with S. salivarius. The majority of isolates from the buccal mucosa (42 of 55) has coaggregation properties like those from the tongue. These results indicate that the three human oral Veillonella species are distributed on oral surfaces that are also occupied by their coaggregation partners and thus provide strong evidence that coaggregation plays a critical role in the bacterial ecology of the oral cavity. 相似文献