全文获取类型
收费全文 | 1934篇 |
免费 | 192篇 |
专业分类
2126篇 |
出版年
2021年 | 18篇 |
2020年 | 14篇 |
2018年 | 19篇 |
2017年 | 16篇 |
2016年 | 32篇 |
2015年 | 57篇 |
2014年 | 55篇 |
2013年 | 69篇 |
2012年 | 100篇 |
2011年 | 84篇 |
2010年 | 46篇 |
2009年 | 57篇 |
2008年 | 81篇 |
2007年 | 72篇 |
2006年 | 83篇 |
2005年 | 78篇 |
2004年 | 55篇 |
2003年 | 58篇 |
2002年 | 89篇 |
2001年 | 76篇 |
2000年 | 80篇 |
1999年 | 52篇 |
1998年 | 23篇 |
1997年 | 26篇 |
1996年 | 20篇 |
1995年 | 17篇 |
1994年 | 21篇 |
1993年 | 17篇 |
1992年 | 51篇 |
1991年 | 47篇 |
1990年 | 40篇 |
1989年 | 39篇 |
1988年 | 39篇 |
1987年 | 30篇 |
1986年 | 28篇 |
1985年 | 35篇 |
1984年 | 26篇 |
1983年 | 20篇 |
1982年 | 19篇 |
1981年 | 17篇 |
1979年 | 27篇 |
1978年 | 12篇 |
1977年 | 24篇 |
1976年 | 19篇 |
1974年 | 14篇 |
1973年 | 11篇 |
1972年 | 18篇 |
1971年 | 17篇 |
1969年 | 21篇 |
1967年 | 12篇 |
排序方式: 共有2126条查询结果,搜索用时 15 毫秒
71.
Raffel TR LeGros RP Love BC Rohr JR Hudson PJ 《International journal for parasitology》2009,39(2):231-241
Acquired immune memory in vertebrates influences transmission and persistence of infections, with consequences for parasite dynamics at both the individual and population levels. The potential impact of acquired immunity is of particular interest for salamanders, whose acquired immune systems are thought to be less effective than those of frogs and other tetrapods. One way to examine the importance of acquired immunity to parasite dynamics at the population level is by examining the relationship between host age and parasite infection intensity. Acquired immunity reduces infection rates in older animals, causing decreased parasite intensity in older age classes and leading to curvilinear age-intensity relationships for persistent parasites and convex age-intensity relationships for transient parasites. We used age-intensity relationships to look for the signature of acquired immunity for 12 parasite taxa of red-spotted newts (Notophthalmus viridescens), using data from a 2-year parasitological survey of six newt populations. We estimated ages from snout-vent length (SVL) based on the relationship between SVL and skeletochronologically-derived ages in a subset of newts. We found evidence of acquired immunity to two parasite taxa, bacterial pathogens and the protist Amphibiocystidium viridescens, whose convex age-intensity relationships could not be easily explained by alternative mechanisms. Our results suggest that the acquired immune response of newts is sufficient to influence the dynamics of at least some parasites. 相似文献
72.
Hrebícek M Mrázová L Seyrantepe V Durand S Roslin NM Nosková L Hartmannová H Ivánek R Cízkova A Poupetová H Sikora J Urinovská J Stranecký V Zeman J Lepage P Roquis D Verner A Ausseil J Beesley CE Maire I Poorthuis BJ van de Kamp J van Diggelen OP Wevers RA Hudson TJ Fujiwara TM Majewski J Morgan K Kmoch S Pshezhetsky AV 《American journal of human genetics》2006,79(5):807-819
Mucopolysaccharidosis IIIC (MPS IIIC, or Sanfilippo C syndrome) is a lysosomal storage disorder caused by the inherited deficiency of the lysosomal membrane enzyme acetyl-coenzyme A: alpha -glucosaminide N-acetyltransferase (N-acetyltransferase), which leads to impaired degradation of heparan sulfate. We report the narrowing of the candidate region to a 2.6-cM interval between D8S1051 and D8S1831 and the identification of the transmembrane protein 76 gene (TMEM76), which encodes a 73-kDa protein with predicted multiple transmembrane domains and glycosylation sites, as the gene that causes MPS IIIC when it is mutated. Four nonsense mutations, 3 frameshift mutations due to deletions or a duplication, 6 splice-site mutations, and 14 missense mutations were identified among 30 probands with MPS IIIC. Functional expression of human TMEM76 and the mouse ortholog demonstrates that it is the gene that encodes the lysosomal N-acetyltransferase and suggests that this enzyme belongs to a new structural class of proteins that transport the activated acetyl residues across the cell membrane. 相似文献
73.
Here we analyzed the influence of salt stress on plant genome stability. Homologous recombination events were detected in transgenic Arabidopsis plants that carried in their genome a beta-glucuronidase recombination marker. Recombination events were scored as blue sectors using a stereo microscope. Exposure to 50 mM salt resulted in a 3.0-fold increase in recombination frequency. To analyze the organ and tissue specificity of recombination events, we examined cross-sections of leaves, stems and roots. We found that nearly 30% of recombination events in plants grown under normal conditions and nearly 50% of events in plants grown on salt were undetected by the conventional method. Most of the recombination events represented a cluster/group of cells (12 on average), although events with single cells were also detected. Recombination events were very frequent in leaf mesophyll cells. On average, individual recombination events located on leaves contained more cells than events located on roots or stems. Analysis of recombination events in cross-sectioned tissue of salt-treated plants revealed a shift in the distribution of recombination events towards the vascular tissue. We discuss the significance of the finding for plant stress physiology. 相似文献
74.
Klaus Herburger Lenka Franková Martina Pičmanová Anzhou Xin Frank Meulewaeter Andrew Hudson Stephen C. Fry 《The Plant journal : for cell and molecular biology》2021,105(6):1549-1565
Certain transglucanases can covalently graft cellulose and mixed-linkage β-glucan (MLG) as donor substrates onto xyloglucan as acceptor substrate and thus exhibit cellulose:xyloglucan endotransglucosylase (CXE) and MLG:xyloglucan endotransglucosylase (MXE) activities in vivo and in vitro. However, missing information on factors that stimulate or inhibit these hetero-transglucosylation reactions limits our insight into their biological functions. To explore factors that influence hetero-transglucosylation, we studied Equisetum fluviatile hetero-trans-β-glucanase (EfHTG), which exhibits both CXE and MXE activity, exceeding its xyloglucan:xyloglucan homo-transglucosylation (XET) activity. Enzyme assays employed radiolabelled and fluorescently labelled oligomeric acceptor substrates, and were conducted in vitro and in cell walls (in situ). With whole denatured Equisetum cell walls as donor substrate, exogenous EfHTG (extracted from Equisetum or produced in Pichia) exhibited all three activities (CXE, MXE, XET) in competition with each other. Acting on pure cellulose as donor substrate, the CXE action of Pichia-produced EfHTG was up to approximately 300% increased by addition of methanol-boiled Equisetum extracts; there was no similar effect when the same enzyme acted on soluble donors (MLG or xyloglucan). The methanol-stable factor is proposed to be expansin-like, a suggestion supported by observations of pH dependence. Screening numerous low-molecular-weight compounds for hetero-transglucanase inhibition showed that cellobiose was highly effective, inhibiting the abundant endogenous CXE and MXE (but not XET) action in Equisetum internodes. Furthermore, cellobiose retarded Equisetum stem elongation, potentially owing to its effect on hetero-transglucosylation reactions. This work provides insight and tools to further study the role of cellulose hetero-transglucosylation in planta by identifying factors that govern this reaction. 相似文献
75.
Assessment of permeable covers for odour reduction in piggery effluent ponds. 2. Field-scale trials 总被引:1,自引:0,他引:1
The efficacy of supported covers was investigated under field conditions using a series of prototypes deployed on an anaerobic pond treating typical piggery waste. Research focused on identifying effective cover support materials and deployment methods, quantifying odour reduction, and estimating the life expectancy of various permeable cover materials. Over a 10-month period, median odour emission rates were five to eight times lower from supported straw cover surfaces and a non-woven, spun fibre polypropylene weed control material than from the adjacent uncovered pond surface. While the straw covers visually appeared to degrade very rapidly, they continued to reduce odour emissions effectively. The polypropylene cover appeared to offer advantages from the perspectives of cost, reduced maintenance and ease of manufacture. 相似文献
76.
Louis Laviolette Marie-Cécile Niérat Anna L. Hudson Mathieu Raux étienne Allard Thomas Similowski 《PloS one》2013,8(4)
Introduction
In humans, cortical mechanisms can interfere with autonomic breathing. Respiratory-related activation of the supplementary motor area (SMA) has been documented during voluntary breathing and in response to inspiratory constraints. The SMA could therefore participate in the increased resting state of the respiratory motor system during wake (i.e. "wakefulness drive to breathe").Methods
The SMA was conditioned by continuous theta burst magnetic stimulation (cTBS, inhibitory) and 5 Hz conventional rTMS (5 Hz, excitatory). The ensuing effects were described in terms of the diaphragm motor evoked response (DiMEPs) to single-pulse transcranial magnetic stimulation over the motor cortex. DiMEPs were recorded at baseline, and at 3 time-points ("post1", "post2", "post3") up to 15 minutes following conditioning of the SMA.Results
cTBS reduced the amplitude of DiMEPs from 327.5±159.8 µV at baseline to 243.3±118.7 µV, 217.8±102.9 µV and 240.6±123.9 µV at post 1, post 2 and post 3, respectively (F = 6.341, p = 0.002). 5 Hz conditioning increased the amplitude of DiMEPs from 184.7±96.5 µV at baseline to 270.7±135.4 µV at post 3 (F = 4.844, p = 0.009).Conclusions
The corticospinal pathway to the diaphragm can be modulated in both directions by conditioning the SMA. This suggests that the baseline respiratory activity of the SMA represents an equipoise from which it is possible to move in either direction. The resting corticofugal outflow from the SMA to phrenic motoneurones that this study evidences could putatively contribute to the wakefulness drive to breathe. 相似文献77.
Seivwright LJ Redpath SM Mougeot F Leckie F Hudson PJ 《Proceedings. Biological sciences / The Royal Society》2005,272(1578):2299-2304
Field studies of mechanisms involved in population regulation have tended to focus on the roles of either intrinsic or extrinsic factors, but these are rarely mutually exclusive and their interactions can be crucial in determining dynamics. Experiments on red grouse Lagopus lagopus scoticus have shown that population instability can be caused both by the effects of a parasitic nematode, Trichostrongylus tenuis, on host production or by changes in testosterone influencing aggressive behaviour and recruitment. We experimentally tested for an interaction between testosterone and T. tenuis in free-living male grouse. A total of 123 grouse were caught in autumn, treated with an anthelmintic to remove parasites, and then given either testosterone or empty, control, implants. After one month grouse were re-infected with a standard dose of parasites. We show that males with increased testosterone levels had greater parasite intensities than controls after one year. We discuss possible physiological and behavioural mechanisms linking testosterone and increased parasite intensity, and the implications for our understanding of complex, unstable population dynamics. 相似文献
78.
79.
80.
H. N. G. Hudson 《BMJ (Clinical research ed.)》1945,2(4421):419-420