全文获取类型
收费全文 | 255篇 |
免费 | 21篇 |
出版年
2024年 | 3篇 |
2023年 | 3篇 |
2022年 | 8篇 |
2021年 | 12篇 |
2020年 | 6篇 |
2019年 | 9篇 |
2018年 | 7篇 |
2017年 | 6篇 |
2016年 | 5篇 |
2015年 | 12篇 |
2014年 | 17篇 |
2013年 | 16篇 |
2012年 | 18篇 |
2011年 | 18篇 |
2010年 | 12篇 |
2009年 | 5篇 |
2008年 | 9篇 |
2007年 | 14篇 |
2006年 | 11篇 |
2005年 | 13篇 |
2004年 | 7篇 |
2003年 | 12篇 |
2002年 | 12篇 |
2001年 | 5篇 |
2000年 | 2篇 |
1998年 | 1篇 |
1997年 | 1篇 |
1996年 | 4篇 |
1995年 | 3篇 |
1994年 | 1篇 |
1993年 | 5篇 |
1992年 | 1篇 |
1991年 | 3篇 |
1990年 | 2篇 |
1989年 | 1篇 |
1988年 | 2篇 |
1983年 | 2篇 |
1982年 | 4篇 |
1981年 | 1篇 |
1980年 | 2篇 |
1977年 | 1篇 |
排序方式: 共有276条查询结果,搜索用时 15 毫秒
51.
52.
Syed Mohsin Bukhari Huda Ahmed Alghamdi Khalil Ur Rehman Shahla Andleeb Shahbaz Ahmad Nimra Khalid 《Saudi Journal of Biological Sciences》2022,29(3):1781-1788
Pheasant reintroduction and conservation efforts have been in place in Pakistan since the 1980 s, yet there is still a scarcity of data on pheasant microbiome and zoonosis. Instead of growing vast numbers of bacteria in the laboratory, to investigate the fecal microbiome, pheasants (green and ring neck pheasant) were analyzed using 16S rRNA metagenomics and using IonS5TMXL sequencing from two flocks more than 10 birds. Operational taxonomic unit (OTU) cluster analysis and phylogenetic tree analysis was performed using Mothur software against the SSUrRNA database of SILVA and the MUSCLE (Version 3.8.31) software. Results of the analysis showed that firmicutes were the most abundant phylum among the top ten phyla, in both pheasant species, followed by other phyla such as actinobacteria and proteobacteria in ring necked pheasant and bacteroidetes in green necked pheasant. Bacillus was the most relatively abundant genus in both pheasants followed by Oceanobacillus and Teribacillus for ring necked pheasant and Lactobacillus for green necked pheasant. Because of their well-known beneficial characteristics, these genus warrants special attention. Bird droppings comprise germs from the urinary system, gut, and reproductive sites, making it difficult to research each anatomical site at the same time. We conclude that metagenomic analysis and classification provides baseline information of the pheasant fecal microbiome that plays a role in disease and health. 相似文献
53.
MM Hill NH Daud CS Aung D Loo S Martin S Murphy DM Black R Barry F Simpson L Liu PF Pilch JF Hancock MO Parat RG Parton 《PloS one》2012,7(8):e43041
Caveolin-1 and caveolae are differentially polarized in migrating cells in various models, and caveolin-1 expression has been shown to quantitatively modulate cell migration. PTRF/cavin-1 is a cytoplasmic protein now established to be also necessary for caveola formation. Here we tested the effect of PTRF expression on cell migration. Using fluorescence imaging, quantitative proteomics, and cell migration assays we show that PTRF/cavin-1 modulates cellular polarization, and the subcellular localization of Rac1 and caveolin-1 in migrating cells as well as PKCα caveola recruitment. PTRF/cavin-1 quantitatively reduced cell migration, and induced mesenchymal epithelial reversion. Similar to caveolin-1, the polarization of PTRF/cavin-1 was dependent on the migration mode. By selectively manipulating PTRF/cavin-1 and caveolin-1 expression (and therefore caveola formation) in multiple cell systems, we unveil caveola-independent functions for both proteins in cell migration. 相似文献
54.
55.
56.
57.
A Chakrabarti S Acharya A K Pal R Huda S Chakrabarti 《Indian journal of experimental biology》1992,30(1):62-64
Induction of differentially stained sister chromatids at G2/M and determination of baseline sister chromatid exchanges (SCEs) in ascites form of mouse sarcoma 180 cell line have been done by in vivo incorporation of 5-bromodeoxyuridine (BrdU) for two consecutive DNA replication cycles. The baseline SCE frequency is 6.24 at log phase of tumour growth. 相似文献
58.
Quantification of photonic localization properties of targeted nuclear mass density variations: Application in cancer‐stage detection
下载免费PDF全文
![点击此处可从《Journal of biophotonics》网站下载免费的PDF全文](/ch/ext_images/free.gif)
Peeyush Sahay Aditya Ganju Huda M. Almabadi Hemendra M. Ghimire Murali M. Yallapu Omar Skalli Meena Jaggi Subhash C. Chauhan Prabhakar Pradhan 《Journal of biophotonics》2018,11(5)
Light localization is a phenomenon which arises due to the interference effects of light waves inside a disordered optical medium. Quantification of degree light localization in optical media is widely used for characterizing degree of structural disorder in that media. Recently, this light localization approach was extended to analyze structural changes in biological cell like heterogeneous optical media, with potential application in cancer diagnostics. Confocal fluorescence microscopy was used to construct “optical lattices,” which represents 2‐dimensional refractive index map corresponding to the spatial mass density distribution of a selected molecule inside the cell. The structural disorder properties of the selected molecules were evaluated numerically using light localization strength in these optical lattices, in a single parameter called “disorder strength.” The method showed a promising potential in differentiating cancerous and non‐cancerous cells. In this paper, we show that by quantifying submicron scale disorder strength in the nuclear DNA mass density distribution, a wide range of control and cancerous breast and prostate cells at different hierarchy levels of tumorigenicity were correctly distinguished. We also discuss how this photonic technique can be used in examining tumorigenicity level in unknown prostate cancer cells, and potential to generalize the method to other cancer cells. 相似文献
59.
Alfred Mansour Larry P. Taylor Jeffrey L. Fine Robert C. Thompson Mary T. Hoversten Henry I. Mosberg Stanley J. Watson Huda Akil 《Journal of neurochemistry》1997,68(1):344-353
Abstract: Structural elements of the rat μ-opioid receptor important in ligand receptor binding and selectivity were examined using a site-directed mutagenesis approach. Five single amino acid mutations were made, three that altered conserved residues in the μ, δ, and κ receptors (Asn150 to Ala, His297 to Ala, and Tyr326 to Phe) and two designed to test for μ/δ selectivity (Ile198 to Val and Val202 to Ile). Mutation of His297 in transmembrane domain 6 (TM6) resulted in no detectable binding with [3 H]DAMGO (3 H-labeled d -Ala2 , N -Me-Phe4 ,Gly-ol5 -enkephalin), [3 H]bremazocine, or [3 H]ethylketocyclazocine. Mutation of Asn150 in TM3 produces a three- to 20-fold increase in affinity for the opioid agonists morphine, DAMGO, fentanyl, β-endorphin1–31 , JOM-13, deltorphin II, dynorphin1–13 , and U50,488, with no change in the binding of antagonists such as naloxone, naltrexone, naltrindole, and nor-binaltorphamine. In contrast, the Tyr326 mutation in TM7 resulted in a decreased affinity for a wide spectrum of μ, δ, and κ agonists and antagonists. Altering Val202 to Ile in TM4 produced no change on ligand affinity, but Ile198 to Val resulted in a four- to fivefold decreased affinity for the μ agonists morphine and DAMGO, with no change in the binding affinities of κ and δ ligands. 相似文献
60.
Amphetamine-, cocaine-, and morphine-induced c-fos expression patterns were examined following an injection protocol that has previously been shown to produce behavioral sensitization
and enhanced dopamine release in the striatal complex. Drug-specific c-fos patterns were observed in both acute and sensitization injection paradigms. A sensitization pretreatment schedule did, however,
alter the c-fos expression patterns induced by all the drugs in the caudate putamen, nucleus accumbens, and the cerebral cortex. In some
striatal and cortical regions, there was an increase or recruitment of cells expressing c-fos whereas in others there was an apparent decrease or inhibition. The somatosensory cortex was one area where pretreatment
with all three drugs increased c-fos expression. The results suggest that the neuronal networks that are modulated by systemic drug injections in the sensitized
animal differ from those affected by the initial drug exposure; areas of overlap may indicate common ‘sensitization’ circuits.
Special issue dedicated to Dr. Eric J. Simon. 相似文献