全文获取类型
收费全文 | 2732篇 |
免费 | 195篇 |
国内免费 | 4篇 |
出版年
2021年 | 38篇 |
2020年 | 32篇 |
2019年 | 24篇 |
2018年 | 29篇 |
2017年 | 28篇 |
2016年 | 46篇 |
2015年 | 68篇 |
2014年 | 108篇 |
2013年 | 130篇 |
2012年 | 174篇 |
2011年 | 153篇 |
2010年 | 103篇 |
2009年 | 102篇 |
2008年 | 147篇 |
2007年 | 153篇 |
2006年 | 136篇 |
2005年 | 127篇 |
2004年 | 131篇 |
2003年 | 108篇 |
2002年 | 98篇 |
2001年 | 30篇 |
2000年 | 37篇 |
1999年 | 37篇 |
1998年 | 42篇 |
1997年 | 24篇 |
1996年 | 35篇 |
1995年 | 28篇 |
1994年 | 25篇 |
1993年 | 33篇 |
1992年 | 44篇 |
1991年 | 31篇 |
1990年 | 30篇 |
1989年 | 29篇 |
1988年 | 29篇 |
1987年 | 20篇 |
1986年 | 19篇 |
1985年 | 32篇 |
1984年 | 33篇 |
1983年 | 17篇 |
1982年 | 20篇 |
1981年 | 28篇 |
1980年 | 21篇 |
1978年 | 24篇 |
1977年 | 19篇 |
1976年 | 20篇 |
1975年 | 22篇 |
1974年 | 23篇 |
1973年 | 17篇 |
1971年 | 15篇 |
1970年 | 15篇 |
排序方式: 共有2931条查询结果,搜索用时 15 毫秒
111.
112.
113.
E Devinoy C Hubert E Schaerer L M Houdebine J P Kraehenbuhl 《Nucleic acids research》1988,16(16):8180
114.
115.
116.
Hui Liu Xiang Li Matthew D. Dun Sam Faulkner Chen Chen Jiang Hubert Hondermarck 《Proteomics》2020,20(10)
Pancreatic cancer has a dismal prognosis and to date there are no targeted therapies for this malignancy. Using shotgun proteomics, the mRNA binding protein cold shock domain containing E1 (CSDE1), also called upstream‐of‐N‐Ras, is detected in pancreatic cancer cell lines but not in normal pancreatic epithelial cells. The expression of CSDE1 in pancreatic cancer cells is confirmed by Western blotting and immunohistochemistry of human pancreatic tumors. In vitro functional assays show that siRNA downregulation of CSDE1 or gene knockout using CRISPR‐Cas9 significantly reduce the invasiveness of pancreatic cancer cells. Together, this study reveals that CSDE1 is overexpressed in pancreatic cancer and is a potential therapeutic target to inhibit pancreatic cancer cell invasion. 相似文献
117.
Emma Bell Angela Sherry Giovanni Pilloni Ana Suárez-Suárez Margaret A. Cramm Guillermo Cueto Ian M. Head Casey R. J. Hubert 《Environmental microbiology》2020,22(1):456-465
Thermophilic endospores are widespread in cold marine sediments where the temperature is too low to support growth and activity of thermophiles in situ. These endospores are likely expelled from warm subsurface environments and subsequently dispersed by ocean currents. The endospore upper temperature limit for survival is 140°C, which can be tolerated in repeated short exposures, potentially enabling transit through hot crustal fluids. Longer-term thermal tolerance of endospores, and how long they could persist in an environment hotter than their maximum growth temperature, is less understood. To test whether thermophilic endospores can survive prolonged exposure to high temperatures, sediments were incubated at 80–90°C for 6, 12 or 463 days. Sediments were then cooled by 10–40°C, mimicking the cooling in subsurface oil reservoirs subjected to seawater injection. Cooling the sediments induced sulfate reduction, coinciding with an enrichment of endospore-forming Clostridia. Different Desulfofundulus, Desulfohalotomaculum, Desulfallas, Desulfotomaculum and Desulfofarcimen demonstrated different thermal tolerances, with some Desulfofundulus strains surviving for >1 year at 80°C. In an oil reservoir context, heat-resistant endospore-forming sulfate-reducing bacteria have a survival advantage if they are introduced to, or are resident in, an oil reservoir normally too hot for germination and growth, explaining observations of reservoir souring following cold seawater injection. 相似文献
118.
Júlia R. de Rezende Thomas B. P. Oldenburg Tetyana Korin William D. L. Richardson Milovan Fustic Carolyn M. Aitken Bernard F. J. Bowler Angela Sherry Alexander Grigoryan Gerrit Voordouw Stephen R. Larter Ian M. Head Casey R. J. Hubert 《Environmental microbiology》2020,22(8):3049-3065
Most of the oil in low temperature, non-uplifted reservoirs is biodegraded due to millions of years of microbial activity, including via methanogenesis from crude oil. To evaluate stimulating additional methanogenesis in already heavily biodegraded oil reservoirs, oil sands samples were amended with nutrients and electron acceptors, but oil sands bitumen was the only organic substrate. Methane production was monitored for over 3000 days. Methanogenesis was observed in duplicate microcosms that were unamended, amended with sulfate or that were initially oxic, however methanogenesis was not observed in nitrate-amended controls. The highest rate of methane production was 0.15 μmol CH4 g−1 oil d−1, orders of magnitude lower than other reports of methanogenesis from lighter crude oils. Methanogenic Archaea and several potential syntrophic bacterial partners were detected following the incubations. GC–MS and FTICR–MS revealed no significant bitumen alteration for any specific compound or compound class, suggesting that the very slow methanogenesis observed was coupled to bitumen biodegradation in an unspecific manner. After 3000 days, methanogenic communities were amended with benzoate resulting in methanogenesis rates that were 110-fold greater. This suggests that oil-to-methane conversion is limited by the recalcitrant nature of oil sands bitumen, not the microbial communities resident in heavy oil reservoirs. 相似文献
119.
Hubert du Pontavice Didier Gascuel Gabriel Reygondeau Aurore Maureaud William W. L. Cheung 《Global Change Biology》2020,26(3):1306-1318
Sea water temperature affects all biological and ecological processes that ultimately impact ecosystem functioning. In this study, we examine the influence of temperature on global biomass transfers from marine secondary production to fish stocks. By combining fisheries catches in all coastal ocean areas and life‐history traits of exploited marine species, we provide global estimates of two trophic transfer parameters which determine biomass flows in coastal marine food web: the trophic transfer efficiency (TTE) and the biomass residence time (BRT) in the food web. We find that biomass transfers in tropical ecosystems are less efficient and faster than in areas with cooler waters. In contrast, biomass transfers through the food web became faster and more efficient between 1950 and 2010. Using simulated changes in sea water temperature from three Earth system models, we project that the mean TTE in coastal waters would decrease from 7.7% to 7.2% between 2010 and 2100 under the ‘no effective mitigation’ representative concentration pathway (RCP8.5), while BRT between trophic levels 2 and 4 is projected to decrease from 2.7 to 2.3 years on average. Beyond the global trends, we show that the TTEs and BRTs may vary substantially among ecosystem types and that the polar ecosystems may be the most impacted ecosystems. The detected and projected changes in mean TTE and BRT will undermine food web functioning. Our study provides quantitative understanding of temperature effects on trophodynamic of marine ecosystems under climate change. 相似文献
120.
Maxime Olmos Mark R. Payne Marie Nevoux Etienne Prvost Grald Chaput Hubert Du Pontavice Jrme Guitton Timothy Sheehan Katherine Mills Etienne Rivot 《Global Change Biology》2020,26(3):1319-1337
A major challenge in understanding the response of populations to climate change is to separate the effects of local drivers acting independently on specific populations, from the effects of global drivers that impact multiple populations simultaneously and thereby synchronize their dynamics. We investigated the environmental drivers and the demographic mechanisms of the widespread decline in marine survival rates of Atlantic salmon (Salmo salar) over the last four decades. We developed a hierarchical Bayesian life cycle model to quantify the spatial synchrony in the marine survival of 13 large groups of populations (called stock units, SU) from two continental stock groups (CSG) in North America (NA) and Southern Europe (SE) over the period 1971–2014. We found strong coherence in the temporal variation in postsmolt marine survival among the 13 SU of NA and SE. A common North Atlantic trend explains 37% of the temporal variability of the survivals for the 13 SU and declines by a factor of 1.8 over the 1971–2014 time series. Synchrony in survival trends is stronger between SU within each CSG. The common trends at the scale of NA and SE capture 60% and 42% of the total variance of temporal variations, respectively. Temporal variations of the postsmolt survival are best explained by the temporal variations of sea surface temperature (SST, negative correlation) and net primary production indices (PP, positive correlation) encountered by salmon in common domains during their marine migration. Specifically, in the Labrador Sea/Grand Banks for populations from NA, 26% and 24% of variance is captured by SST and PP, respectively and in the Norwegian Sea for populations from SE, 21% and 12% of variance is captured by SST and PP, respectively. The findings support the hypothesis of a response of salmon populations to large climate‐induced changes in the North Atlantic simultaneously impacting populations from distant continental habitats. 相似文献