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171.
Shu-Yu Yu Bao-Lin Mao Ping Xiao Wei-Ping Yu Ying-Long Wang Chang-Zhi Huang Wang-Qiu Chen Xiang-Zhan Xuan 《Biological trace element research》1990,24(2-3):105-108
This pilot study evaluated the feasibility and effectiveness of conducting a double-blind clinical trial for the prevention of lung cancer with selenium (Se) in Yunnan Tin Corporation, the People's Republic of China, where the incidence rates of lung cancer are extraordinarily high among the miners. Forty healthy miners were randomized to either 300 μg of Se in high Se malt cakes or an identical placebo of malt cakes daily for one year. Subjects consumed their usual daily diet. The low Se concentrations in plasma (0.05±0.008 μg/mL) and hair (0.442±0.085 μg/g) reflected their low dietary Se intake in the control subjects. In Se-supplemented group, the Se status was increased by 178% for serum and 194.8% for hair. The serum GSHpx activity was increased by 155.7%, whereas the lipid peroxide level was reduced by 74.5% compared to the placebo. The results of UDS assay indicated that the lymphocyte DNA damage induced by ultraviolet irradiation and carcinogen 3,4-benzpyrene could be protected by Se supplementation. Se-supplementation did not affect the liver function test (SGPT), as well as the concentrations of hemoglobin, albumin, and cholesterol. Thus, daily intake of 300 μg Se in form of Se-malt as a chemopreventive measure is safe and effective to humans with low Se status. 相似文献
172.
P L Kam T M Huang M S Shiao L J Lin G G Chang 《Journal of biochemical and biophysical methods》1990,21(2):115-127
Treatment of 3-aminopyridine adenine dinucleotide phosphate with sodium periodate in the neutral pH resulted in oxidation of the ribose linked to 3-aminopyridine and cleavage of the dinucleotide into adenosine- and 3-aminopyridine-containing moieties. Separation of these moieties was afforded by thin-layer chromatography, high-performance liquid chromatography, and fast protein liquid chromatography. From fast atom bombardment mass spectra and nuclear magnetic resonance spectra, the adenosine-containing moiety was identified as 2'-phosphoadenosine 5'-phosphate while the aminopyridine moiety was present in a mixture of the hydrated 3-aminopyridine mononucleotide/nucleoside dialdehyde. Separation of the completely oxidized product by Pharmacia fast protein liquid chromatography gave three major peaks corresponding to 2'-phosphoadenosine 5'-phosphate, 2'-phosphoadenosine 5'-diphosphate and oxidized 3-aminopyridine nucleoside, with minor amount of oxidized 3-aminopyridine mononucleotide. Thus the oxidized 3-aminopyridine adenine dinucleotide phosphate was shown to cleave by two pathways: it may either undergo beta-elimination to give 2'-phosphoadenosine 5'-diphosphate and oxidized 3-aminopyridine nucleoside; or the phosphodiester linkage may be hydrolyzed to give 2'-phosphoadenosine 5'-phosphate and oxidized 3-aminopyridine mononucleotide. The latter compound may further undergo beta-elimination and eventually give oxidized 3-aminopyridine nucleoside. Hydrolysis could be prevented by storing the sample as lyophilized powder, while beta-elimination was diminished by lowering the storage temperature. We found that the lyophilized powder of oxidized 3-aminopyridine adenine dinucleotide phosphate can be stored at -50 degrees C for several months with minimum decomposition. 相似文献
173.
F M Robertson S K Gilmour A J Beavis S M O'Connell A H Conney M T Huang J D Laskin O A Hietala T G O'Brien 《Cytometry》1990,11(7):832-836
Using flow cytometry in combination with membrane permeabilization techniques to enhance binding of antibodies with immunoreactive protein within the cytoplasm, we have developed a method to examine the ornithine decarboxylase (ODC) activity present within subpopulations of epidermal cells following acute and chronic exposure to the phorbol ester tumor promoter 12-0-tetradecanoylphorbol-13-acetate (TPA). The method described has the sensitivity to detect basal levels of ODC as well as increases in ODC at early time points following treatment with TPA and has the additional advantage of allowing subpopulation identification and characterization. 相似文献
174.
壤白蚁属Parahy potermes Zhu et Huang,新属 模式种:曼允壤白蚁Parahy potermes manyunensis,新种。 属征:兵蚁头近卵圆形,头背面被毛稀少,两侧缘于触角前骤然狭缩,头中部最宽,后缘宽圆,头背面中部明显隆起;左上颚内缘中部第1缘齿上方具2—3枚小齿。 比较与讨论:本新属与地白蚁属Hypotermes Holmgren较接近。但Hypotermes头两侧缘触角前不呈狭缩状;左上颚第1缘齿上方不具锯齿状小齿;头背面中部呈弧状隆起。 相似文献
175.
176.
.熊治廷;.黄德世;.王徽勤;.孙祥钟 《武汉植物学研究》1990,8(1):47-52
用R聚类分析和主分量分析,定量研究了湖北菱科5种8变种37个形态和生态性状的变异及分类学价值。结果表明,植物体大小变异是形态演化的主要方向之一。形态演化的同时,也发生生态分化。除少数性状例外,形态演化与生态分化在相当程度上是独立的。 相似文献
177.
中华猕猴桃胚乳植株后代的观察 总被引:2,自引:0,他引:2
对438株定植的中华猕猴桃胚乳培.养的试管苗,经四年的田间观察,并进行连续二年结果分析。与对照的母株相比,胚乳植株在株形、叶片大小、果实形态及果实的主要营养成分含量上都有较大的变化。同时还发现,由同一块愈伤组织诱导的胚乳试管苗后代中也有雌、雄性别的分化。胚乳植株后代的多样性,可为中华猕猴桃的选种及品种繁育提供丰富的材料。 相似文献
178.
179.
从猪脑中提取钙调蛋白和突触质膜,我们研究了山莨菪碱对经有限蛋白水解和磷脂酶A_2处理后的突触膜Ca~(2+)-ATPase活性影响。发现药物对不同预处理后的Ca~(2+)-ATPase表现出不同影响并调节钙调蛋白对它的激活作用。 相似文献
180.