全文获取类型
收费全文 | 5235篇 |
免费 | 391篇 |
国内免费 | 485篇 |
专业分类
6111篇 |
出版年
2024年 | 9篇 |
2023年 | 73篇 |
2022年 | 175篇 |
2021年 | 313篇 |
2020年 | 208篇 |
2019年 | 229篇 |
2018年 | 254篇 |
2017年 | 181篇 |
2016年 | 234篇 |
2015年 | 316篇 |
2014年 | 391篇 |
2013年 | 394篇 |
2012年 | 482篇 |
2011年 | 429篇 |
2010年 | 274篇 |
2009年 | 265篇 |
2008年 | 277篇 |
2007年 | 252篇 |
2006年 | 194篇 |
2005年 | 145篇 |
2004年 | 161篇 |
2003年 | 134篇 |
2002年 | 92篇 |
2001年 | 76篇 |
2000年 | 80篇 |
1999年 | 74篇 |
1998年 | 43篇 |
1997年 | 46篇 |
1996年 | 47篇 |
1995年 | 40篇 |
1994年 | 29篇 |
1993年 | 27篇 |
1992年 | 26篇 |
1991年 | 17篇 |
1990年 | 17篇 |
1989年 | 19篇 |
1988年 | 8篇 |
1987年 | 9篇 |
1986年 | 9篇 |
1985年 | 10篇 |
1984年 | 6篇 |
1983年 | 3篇 |
1979年 | 8篇 |
1975年 | 7篇 |
1974年 | 4篇 |
1971年 | 4篇 |
1970年 | 4篇 |
1969年 | 2篇 |
1966年 | 3篇 |
1965年 | 3篇 |
排序方式: 共有6111条查询结果,搜索用时 0 毫秒
81.
新疆乌鲁木齐HIV—1流行毒株膜蛋白基因C2—V3区序列测定和亚型分析 总被引:10,自引:0,他引:10
应用PCR方法对7份1996年3-5月采集于新疆乌鲁木齐HIV-1阳性静脉吸毒者的外周血单核细胞样品进行扩增,获得了HIV-1膜蛋白基因的核酸片段,并对其C2-V3区及邻区350-450个核苷酸序列进行了测定和分析。 相似文献
82.
83.
Xinyue Ye Jian Wang Pengfei Xu Xiaoqian Yang Qixue Shi Genyan Liu Zhaoshi Bai Changlin Zhou Lingman Ma 《Microbial biotechnology》2023,16(9):1755-1773
Polymyxin resistance is conferred by MCR-1 (mobile colistin resistance 1)-induced lipopolysaccharide (LPS) modification of G− bacteria. However, the peptide MSI-1 exerts potent antimicrobial activity against mcr-1-carrying bacteria. To further investigate the potential role of MCR-1 in improving bacterial virulence and facilitating immune evasion, and the immunomodulatory effect of peptide MSI-1, we first explored outer membrane vesicle (OMV) alterations of mcr-1-carrying bacteria in the presence and absence of sub-MIC MSI-1, and host immune activation during bacterial infection and OMV stimulation. Our results demonstrated that LPS remodelling induced by MCR-1 negatively affected OMV formation and protein cargo by E. coli. In addition, MCR-1 diminished LPS-stimulated pyroptosis but facilitated mitochondrial dysfunction, further aggravating apoptosis in macrophages induced by OMVs of E. coli. Similarly, TLR4-mediated NF-κB activation was markedly alleviated once LPS was modified by MCR-1. However, peptide MSI-1 at the sub-MIC level inhibited the expression of MCR-1, further partly rescuing OMV alteration and attenuation of immune responses in the presence of MCR-1 during both infection and OMV stimulation, which can be exploited for anti-infective therapy. 相似文献
84.
Abstract The ecdysteroid levels in hemolymph, ovary, synganglion and whole body of diapausing female Dermacentor niveus were detected by HPLC, and compared with the results of nondiapausing female. It is revealed that the ecdysteroid levels in hemolymph and ovary of diapausing female are similar basically to that of nondiapausing female in the first few days after engorgement. From the 10th day after engorgement, the ecdysteroid levels of diapausing female decreased and even became distinctly lower than that of nondiapausing female. The paucity of ecdysteroids in these individuals would influence the normal development of oocytes. In order to explore the effect of ecdyateroids on the diapausing female, we injected 20-hydroxyecdysone with different dosages at different time into the ticks, and found that after just complete engorgement the injection with large dosages (10000 and 1375 ng/tick) caused death of the ticks. From 10th to 20th day after engorgement the ecdysteroid levels of diapausing female are lower than that of nondiapausing one before oviposition, the injection with certain dosages 50, 70 and 100 ng/tick> of 20E can accelerate vitellogenesis and terminate reproductive diapause, but the amount of eggs produced by them is less than that produced by nondiapausing female. The termination of diapause in female of ixcdid tick by exogenous ecdysteroids is reported for the first time. 相似文献
85.
Zhongjie Bai Jinlong Zhang Qiuping Zhang Yanni Wang Jili Li Quanyi Zhao Zhen Wang Dian He Jingke Zhang Bin Liu 《Bioorganic & medicinal chemistry》2019,27(15):3307-3318
Based on the structures of nicotinic acid and chlorfibrate, a series of new H2S donors were synthesized and their anti-atherosclerosis activities using Ox-LDL RAW 264.6 cells as model were evaluated. The release test showed that all the compounds could release H2S effectively and showed low cytotoxicity. In the bioactivity experiments, compounds 1, 3, 9 and 14 increased the survival rate of HUVEC cells treated by ox-LDL; among four compounds, compounds 1 and 3 displayed higher activity than the others. In the foam cell model, compounds 1 and 3 were found to inhibit the formation of foam cells and significantly reduced the content of TC and FC in foam cells. They had more obvious effects on lipid reduction than those of nicotinic acid and chlorfibrate. In anti-oxidation, compounds 1 and 3 significantly reduced ROS and MDA and increased the expression level of SOD, whereas the precursor compounds, niacin and chlorfibrate had little antioxidant effect. In addition, both compounds also inhibited the inflammatory response in foam cells, with reducing pro-inflammatory factor TNF-α and increasing anti-inflammatory cytokine IL-10. WB assay showed that the tested compounds inhibited the expression levels PI3K, Akt and NF-κb proteins. In conclusion, the compounds as H2S donors could protect HUVEC cells from damage and inhibit the formation of foam cells by inhibiting PI3K/Akt/NF-κb signal pathway. All these suggest the compounds have potential to be candidate for anti-atherosclerosis medicines. 相似文献
86.
Rongquan Lai Hanqing Hu Xiaoting Wu Jingjing Bai Gang Gu Jianbao Bai Ting Zhou Tianran Lin Xiujin Zhong 《Entomologia Experimentalis et Applicata》2019,167(11):969-976
Tobacco viruses transmitted by green peach aphids, Myzus persicae (Sulzer) (Hemiptera: Aphididae), cause severe disease in flue‐cured tobacco, Nicotiana tabacum L. (Solanaceae), in China and throughout the world. Field experiments were conducted in 2016 and 2017 in Longyan City, Fujian Province, China, to determine whether M. persicae and aphid‐transmitted virus diseases are affected by intercropping of oilseed rape, Brassica napus L. (Brassicaceae), in tobacco fields. The results showed that, compared with those in monocultured fields, the densities of M. persicae and winged aphids in intercropped fields significantly decreased in both 2016 and 2017. In particular, the appearance of winged aphids was delayed by ca. 7 days. Moreover, the densities of Aphidius gifuensis Ashmead (Hymenoptera: Aphidiidae), a parasitoid of the aphid, significantly increased in 2016 and 2017. Accordingly, the incidence rates of aphid‐transmitted virus diseases (those caused by the cucumber mosaic virus, potato virus Y, and tobacco etch virus) significantly decreased in the intercropped fields in 2016 and 2017. Tobacco yields and monetary value significantly increased in 2016 (by 10–25 and 14–29%, respectively) and 2017 (by 17–22 and 22–34%, respectively). Consequently, our results suggest that intercropping oilseed rape in tobacco fields is a good approach to regulating and controlling aphids and tobacco mosaic viruses, for example potyvirus, and this intercropping can help control aphid‐transmitted virus diseases in tobacco. 相似文献
87.
肾性和自发性高血压大鼠心肌肥大前后心肌Gαq/11含量的变化 总被引:1,自引:0,他引:1
目的 :探讨Gαq/11在不同原因所致心肌肥大中的变化。方法 :两肾一夹肾性高血压大鼠 (RHR)和自发性高血压大鼠(SHR)模型 ,测定动脉血压和心肌肥大指数 ,放免法测定心肌血管紧张素II(AngII)含量 ,免疫印迹法测定心肌Gαq/11含量。 结果 :RHR术后 1周动脉血压、心肌肥大指数及Gαq/11含量与假手术组无差异 ,心肌AngII含量显著升高 (P <0 .0 1) ;术后 8周上述各指标均较假手术组升高。 12周龄SHR动脉血压、心肌肥大指数和AngⅡ含量均较同龄WKY升高 (P均 <0 .0 1) ,但心肌Gαq/11含量却无明显变化 ;4周龄时上述各指标与同龄对照相比均无明显差异。 结论 :Gαq/11在肾性和自发性高血压心肌肥大中有不同变化。 相似文献
88.
89.
90.
SnRK2基因对植物的逆境胁迫具有重要的调节作用,以马铃薯‘陇薯3号’(Solanum tuberosum)为试材,采用RT-PCR方法从马铃薯试管苗中克隆得到1个SnRK2.1基因cDNA,命名为StSnRK2.1,提交GenBank注册,注册号为JX280911。通过生物信息学分析,该基因开放阅读框全长1 008 bp,编码335个氨基酸。预测蛋白质分子量约为37.77 kD,等电点为5.37,蛋白质二级结构预测α-螺旋42.39%,延伸链16.42%,β-折叠7.46%,无规卷曲33.73%,具有疏水性,为膜内蛋白。亚细胞定位显示该基因出现在细胞质及微体中的可能性较大。肽链可能有7处丝氨酸磷酸化位点,2处苏氨酸磷酸化位点,以及3处酪氨酸磷酸化位点,因此推测该基因在植物抗逆中有重要的作用。 相似文献