全文获取类型
收费全文 | 55212篇 |
免费 | 4574篇 |
国内免费 | 3971篇 |
出版年
2024年 | 117篇 |
2023年 | 696篇 |
2022年 | 1599篇 |
2021年 | 2741篇 |
2020年 | 1777篇 |
2019年 | 2153篇 |
2018年 | 2013篇 |
2017年 | 1532篇 |
2016年 | 2225篇 |
2015年 | 3304篇 |
2014年 | 3840篇 |
2013年 | 4199篇 |
2012年 | 4787篇 |
2011年 | 4559篇 |
2010年 | 2653篇 |
2009年 | 2471篇 |
2008年 | 2799篇 |
2007年 | 2463篇 |
2006年 | 2219篇 |
2005年 | 1813篇 |
2004年 | 1597篇 |
2003年 | 1435篇 |
2002年 | 1222篇 |
2001年 | 1094篇 |
2000年 | 940篇 |
1999年 | 909篇 |
1998年 | 595篇 |
1997年 | 582篇 |
1996年 | 549篇 |
1995年 | 482篇 |
1994年 | 445篇 |
1993年 | 372篇 |
1992年 | 525篇 |
1991年 | 441篇 |
1990年 | 381篇 |
1989年 | 294篇 |
1988年 | 274篇 |
1987年 | 224篇 |
1986年 | 158篇 |
1985年 | 207篇 |
1984年 | 139篇 |
1983年 | 113篇 |
1982年 | 98篇 |
1981年 | 71篇 |
1980年 | 62篇 |
1979年 | 82篇 |
1978年 | 76篇 |
1977年 | 53篇 |
1976年 | 52篇 |
1973年 | 55篇 |
排序方式: 共有10000条查询结果,搜索用时 31 毫秒
21.
22.
RNA‐seq analysis reveals new candidate genes for drip loss in a Pietrain × Duroc × Landrace × Yorkshire population
下载免费PDF全文
![点击此处可从《Animal genetics》网站下载免费的PDF全文](/ch/ext_images/free.gif)
Bojiang Li Kaiqing Liu Qiannan Weng Pinghua Li Wei Wei Qifa Li Jie Chen Ruihua Huang Wangjun Wu Honglin Liu 《Animal genetics》2016,47(2):192-199
Drip loss, one of the most important meat quality traits, is characterized by low heritability. To date, the genetic factors affecting the drip loss trait have not been clearly elucidated. The objective of this study was to identify critical candidate genes affecting drip loss. First, we generated a Pietrain × Duroc × Landrace × Yorkshire commercial pig population and obtained phenotypic values for the drip loss trait. Furthermore, we constructed two RNA libraries from pooled samples of longissimus dorsi muscles with the highest (H group) and lowest (L group) drip loss and identified the differentially expressed genes (DEGs) between these extreme phenotypes using RNA‐seq technology. In total, 25 883 genes were detected in the H and L group libraries, and none was specifically expressed in only one library. Comparative analysis of gene expression levels found that 150 genes were differentially expressed, of which 127 were upregulated and 23 were downregulated in the H group relative to the L group. In addition, 68 drip loss quantitative trait loci (QTL) overlapping with 63 DEGs were identified, and these QTL were distributed mainly on chromosomes 1, 2, 5 and 6. Interestingly, the triadin (TRDN) gene, which is involved in muscle contraction and fat deposition, and the myostatin (MSTN) gene, which has a role in muscle growth, were localized to more than two drip loss QTL, suggesting that both are critical candidate genes responsible for drip loss. 相似文献
23.
24.
25.
Many animals exhibit different behaviors in different seasons. The photoperiod can have effects on migration, breeding, fur growth, and other processes. The cyclic growth of the fur and feathers of some species of mammals and birds, respectively, is stimulated by the photoperiod as a result of hormone-dependent regulation of the nervous system. To further examine this phenomenon, we evaluated the Arbas Cashmere goat (Capra hircus), a species that is often used in this type of research. The goats were exposed to an experimentally controlled short photoperiod to study the regulation of cyclic cashmere growth. Exposure to a short photoperiod extended the anagen phase of the Cashmere goat hair follicle to increase cashmere production. Assessments of tissue sections indicated that the short photoperiod significantly induced cashmere growth. This conclusion was supported by a comparison of the differences in gene expression between the short photoperiod and natural conditions using gene chip technology. Using the gene chip data, we identified genes that showed altered expression under the short photoperiod compared to natural conditions, and these genes were found to be involved in the biological processes of hair follicle growth, structural composition of the hair follicle, and the morphogenesis of the surrounding skin appendages. Knowledge about differences in the expression of these genes as well as their functions and periodic regulation patterns increases our understanding of Cashmere goat hair follicle growth. This study also provides preliminary data that may be useful for the development of an artificial method to improve cashmere production by controlling the light cycle, which has practical significance for livestock breeding. 相似文献
26.
27.
The mechanism of mass collaboration in risk management was studied during the Sichuan earthquake under a Web-based “PeopleFinder” project, where information is contributed and shared among mass contributors. The case study is provided by a great earthquake that happened in Wenchuan County, Sichuan Province, of southwestern China at 2:28 p.m. on May 12, 2008. We witnessed and experienced the rescue and relief efforts for the great earthquake. In this article, two fundamental frameworks are developed to study the mechanism of mass collaboration. Mass collaboration is proven to be effective in a big public crisis such as the Sichuan earthquake. 相似文献
28.
It is still a difficult clinical issue to decide whether a patient is a suitable candidate for a cochlear implant and to plan postoperative rehabilitation, especially for some special cases, such as auditory neuropathy. A partial solution to these problems is to preoperatively evaluate the functional integrity of the auditory neural pathways. For evaluating the strength of phase-locking of auditory neurons, which was not reflected in previous methods using electrically evoked auditory brainstem response (EABR), a new method for recording phase-locking related auditory responses to electrical stimulation, called the electrically evoked frequency-following response (EFFR), was developed and evaluated using guinea pigs. The main objective was to assess feasibility of the method by testing whether the recorded signals reflected auditory neural responses or artifacts. The results showed the following: 1) the recorded signals were evoked by neuron responses rather than by artifact; 2) responses evoked by periodic signals were significantly higher than those evoked by the white noise; 3) the latency of the responses fell in the expected range; 4) the responses decreased significantly after death of the guinea pigs; and 5) the responses decreased significantly when the animal was replaced by an electrical resistance. All of these results suggest the method was valid. Recording obtained using complex tones with a missing fundamental component and using pure tones with various frequencies were consistent with those obtained using acoustic stimulation in previous studies. 相似文献
29.
30.