全文获取类型
收费全文 | 11924篇 |
免费 | 1270篇 |
国内免费 | 2732篇 |
出版年
2024年 | 47篇 |
2023年 | 166篇 |
2022年 | 332篇 |
2021年 | 503篇 |
2020年 | 397篇 |
2019年 | 558篇 |
2018年 | 491篇 |
2017年 | 442篇 |
2016年 | 524篇 |
2015年 | 731篇 |
2014年 | 912篇 |
2013年 | 936篇 |
2012年 | 1128篇 |
2011年 | 1099篇 |
2010年 | 837篇 |
2009年 | 786篇 |
2008年 | 872篇 |
2007年 | 817篇 |
2006年 | 726篇 |
2005年 | 625篇 |
2004年 | 502篇 |
2003年 | 512篇 |
2002年 | 453篇 |
2001年 | 375篇 |
2000年 | 249篇 |
1999年 | 172篇 |
1998年 | 110篇 |
1997年 | 85篇 |
1996年 | 64篇 |
1995年 | 52篇 |
1994年 | 52篇 |
1993年 | 46篇 |
1992年 | 55篇 |
1991年 | 36篇 |
1990年 | 31篇 |
1989年 | 37篇 |
1988年 | 25篇 |
1987年 | 21篇 |
1986年 | 20篇 |
1985年 | 23篇 |
1984年 | 14篇 |
1983年 | 17篇 |
1982年 | 16篇 |
1981年 | 6篇 |
1980年 | 3篇 |
1973年 | 2篇 |
1972年 | 2篇 |
1959年 | 3篇 |
1956年 | 2篇 |
1950年 | 3篇 |
排序方式: 共有10000条查询结果,搜索用时 93 毫秒
61.
62.
蝮蛇毒碱性磷脂酶A_2基因的克隆 总被引:2,自引:0,他引:2
从蝮蛇毒腺中抽提总RNA.利用人工合成寡核苷酸引物作逆转录,以cDNA为模板进行体外扩增,获得磷脂酶A2(简称PLA2)基因,克隆至pBS-ks载体中。通过对3个碱性PLA2(简称BPLA2)基因单独克隆分别作DNA全序列分析,推导pro-BPLA2由138个氨基酸残基构成,与已测定的部分氨基酸序列比较,基本相符。该基因成功的克隆,不仅推导出BPLA2的蛋白质全序列,也为进一步开展蛇毒功能肽蛋白质工程的研究工作打下了良好的基础。 相似文献
63.
中国异皮属线虫一新种(线虫纲:垫刃目:异皮科) 总被引:1,自引:0,他引:1
本文描述采自河北省滦县寄生于白茅Imperate cylindrica var.Major的异皮属线虫一新种,中华异皮线虫Heterodera sinensis sp.nov.;新种孢囊为柠檬形,阴门膜孔为两侧单膜孔型,下桥发达有泡状突;二龄幼虫口针基部球前部略凹陷,口孔侧唇外缘融合王头晕第1环纹内,即无明显侧唇,其体侧区有3条侧线与体环纹呈不完全网格状交叉;雄虫侧区有3条侧线与体环纹呈完全网格状 相似文献
64.
西双版纳石灰岩森林的植物区系地理研究 总被引:20,自引:7,他引:13
西双版纳石灰岩森林植物区系经调查有维管束植物153科,640属,1394种及变种,其中,种子植物占129科558属1269种及变种。种子植物的分布区类型组成是热带和主产热带的科占总科数的71.3%;热带分布属占总属数的90.1%;热带分布种超过总种数的90%。热带分布属中又以热带亚洲分布属最多,占总数的35.3%;热带分布种中则以热带亚洲分布及其变型的种类占总种数的64.5%为特点。这表明该石灰岩森林植物区系是热带性质的植物区系,属于热带亚洲区系的一部分。由于特殊的地理位置,西双版纳地区是许多典型热带植物的分布北界,同时又是几种地理成分的交汇地带,这又使该石灰岩区系带有明显热带边缘性质和多种地理成分交汇的特点。 相似文献
65.
栝楼核糖核酸酶(RNase TCS)对U碱基具有高度的专一性,在无脲、pH3.5、50℃时,它几乎都在-NP ↓ U-处裂解RNA.它与RNase T1,U2和有限的碱水解一起,可用于直接的酶法RNA序列分析. 相似文献
66.
根据14丘稻田稻茎毛眼水蝇幼虫调查资料.对其幼虫分布型进行了分析。结果表明;用I、CAm/m、Iσ四种聚集指标法测定,64.3%的田块呈随机分布.28.4%为均匀分布.7.3%为聚集分布。用Iwao平均拥挤度和Taylor幂法则测定.其田间分布型符合随机分布。用频次拟合法测验,50%的田块同时符合渡松、奈曼和负二项分布三种分布型;35.7%的田块同时符合2种分布型。根据该虫田间分布型的特点.制定了相应的序贯抽样技术。 相似文献
67.
A Role for Cdk2 Kinase in Negatively Regulating DNA Replication during S Phase of the Cell Cycle 总被引:17,自引:4,他引:13 下载免费PDF全文
Using cell-free extracts made from Xenopus eggs, we show that cdk2-cyclin E and A kinases play an important role in negatively regulating DNA replication. Specifically, we demonstrate that the cdk2 kinase concentration surrounding chromatin in extracts increases 200-fold once the chromatin is assembled into nuclei. Further, we find that if the cdk2–cyclin E or A concentration in egg cytosol is increased 16-fold before the addition of sperm chromatin, the chromatin fails to initiate DNA replication once assembled into nuclei. This demonstrates that cdk2–cyclin E or A can negatively regulate DNA replication. With respect to how this negative regulation occurs, we show that high levels of cdk2–cyclin E do not block the association of the protein complex ORC with sperm chromatin but do prevent association of MCM3, a protein essential for replication. Importantly, we find that MCM3 that is prebound to chromatin does not dissociate when cdk2– cyclin E levels are increased. Taken together our results strongly suggest that during the embryonic cell cycle, the low concentrations of cdk2–cyclin E present in the cytosol after mitosis and before nuclear formation allow proteins essential for potentiating DNA replication to bind to chromatin, and that the high concentration of cdk2–cyclin E within nuclei prevents MCM from reassociating with chromatin after replication. This situation could serve, in part, to limit DNA replication to a single round per cell cycle. 相似文献
68.
Human immunodeficiency virus types 1 and 2 and simian immunodeficiency virus Nef use distinct but overlapping target sites for downregulation of cell surface CD4. 总被引:3,自引:0,他引:3 下载免费PDF全文
Although the Nef proteins encoded by human immunodeficiency virus type 1 (HIV-1) and simian immuno-deficiency virus (SIV) are known to induce the efficient internalization and degradation of cell surface CD4, it remains unclear whether this process involves a direct interaction between Nef and CD4. Here, we report that CD4 downregulation by HIV-1 and SIV Nef requires distinct but overlapping target sites within the CD4 intracytoplasmic domain. In particular, mutation of a glutamic acid residue located at CD4 residue 405 or of arginine and methionine residues located, respectively, at residue 406 and 407 results in a mutant CD4 protein that is efficiently downregulated by HIV-1 Nef but refractory to downregulation by SIV Nef. However, both HIV-1 and SIV Nef require an isoleucine located at residue 410 and the dileucine motif found at CD4 residues 413 and 414. CD4 downregulation induced by the Nef protein encoded by HIV-2 is shown to require a CD4 target sequence that is similar to, but distinct from, that observed with SIV Nef. These data explain the previous finding that the murine CD4 protein, which has an alanine at residue 405, is refractory to downregulation by SIV, but not HIV-1, Nef (J. L. Foster, S.J. Anderson, A. L. B. Frazier, and J. V. Garcia, Virology 201:373-379, 1994). In addition, these observations provide strong genetic support for the hypothesis that the Nef-mediated downregulation of cell surface CD4 requires a direct Nef-CD4 interaction. 相似文献
69.
Sequence variation and genetic diversity in the giant panda 总被引:3,自引:0,他引:3
ZHANG YapingOliver A. RyderFAN Zhiyong ZHANG HemingHE TingmeiHE Guangxin ZHANG Anju FEI LisongZHONG Shunlong CHEN HongZHANG Chenglin YANG Minghai ZHU Feibing PENG Zhenxin PU Tianchun CHEN Yucun YAO OMinda GUO Wei 《中国科学:生命科学英文版》1997,40(2):210-216
About 336–444 bp mitochondrial D-loop region and tRNA gene were sequenced for 40 individuals of the giant panda which were
collected from Mabian, Meigu, Yuexi, Baoxing, Pingwu, Qingchuan, Nanping and Baishuijiang, respectively. 9 haplotypes were
found in 21 founders. The results showed that the giant panda has low genetic variations, and that there is no notable genetic
isolation among geographical populations. The ancestor of the living giant panda population perhaps appeared in the late Pleistocene,
and unfortunately, might have suffered bottleneck attacks. Afterwards, its genetic diversity seemed to recover to some extent.
Project supported by the “8.5” Key Project of Chinese Academy of Sciences, the Chairman Foundation of Chinese Academy of Sciences,
K. C. Wang Education Foundation, the Applied Basic Research Foundation of Yunnan, the National Natural Science Foundation
of China, the Special Foundation for Returned Chinese Scientists, and Zoological Society of San Diego. 相似文献
70.
The dissociation constants for the binding of ferric enterobactin with FepA and FecA are quantitated with displacement experiments. It is found that K
d for FepA is 12 times lower than the one for FecA. This indicates that FepA is an high-affinity receptor while FecA binds ferric enterobactin with a lower affinity. Monoclonal antibodies specific for binding epitopes of FepA inhibit the binding of ferric enterobactin with purified FepA. These same antibodies do not inhibit the binding of ferric enterobactin with purified FecA. This indicates that the binding epitopes in FecA and FepA are different. 相似文献