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261.
Design, expression, and characterization of a novel targeted plectasin against methicillin-resistant Staphylococcus aureus 总被引:1,自引:0,他引:1
Ruoyu Mao Da Teng Xiumin Wang Di Xi Yong Zhang Xiaoyuan Hu Yalin Yang Jianhua Wang 《Applied microbiology and biotechnology》2013,97(9):3991-4002
A novel specifically targeted antimicrobial peptide (STAMP) that was especially effective against methicillin-resistant Staphylococcus aureus (MRSA) was designed by fusing the AgrD1 pheromone to the N-terminal end of plectasin. This STAMP was named Agplectasin, and its gene was synthesized and expressed in Pichia pastoris X-33 via pPICZαA. The highest amount of total secreted protein reached 1,285.5 mg/l at 108 h during the 120-h induction. The recombinant Agplectasin (rAgP) was purified by cation exchange chromatography and hydrophobic exchange chromatography; its yield reached 150 mg/l with 94 % purity. The rAgP exhibited strong bactericidal activity against S. aureus but not Staphylococcus epidermidis or other types of tested bacteria. A bactericidal kinetics assay showed that the rAgP killed over 99.9 % of tested S. aureus (ATCC 25923 and ATCC 43300) in both Mueller–Hinton medium and human blood within 10 h when treated with 4× minimal inhibitory concentration. The rAgP caused only approximately 1 % hemolysis of human blood cells, even when the concentration reached 512 μg/ml, making it potentially feasible as a clinical injection agent. In addition, it maintained a high activity over a wide range of pH values (2.0–10.0) and demonstrated a high thermal stability at 100 °C for 1 h. These results suggested that this STAMP has the potential to eliminate MRSA strains without disrupting the normal flora. 相似文献
262.
纤维素降解菌L-06的筛选、鉴定及其产酶条件的分析 总被引:3,自引:0,他引:3
从用于堆肥的水稻秸秆中初筛出一株高效纤维素降解菌L-06, 根据18S rRNA基因序列和菌株形态分析, 初步鉴定该菌为斜卧青霉(Penicillium decumbens)。研究了液体发酵培养基中氮源、碳源、表面活性剂、培养温度、起始pH以及接种量对该菌株各纤维素酶活力的影响。在最适条件下, 该菌的b-葡萄糖苷酶(BGL)、外切纤维素酶(CBH)于培养第3天酶活力分别达到1662 u/mL和2770 u/mL; 内切纤维素酶(EG)、滤纸糖化力(FPase)于培养第4天酶活力分别达到18064 u/mL和4035 u/mL。在产酶优化实验中, 该菌的EG和CBH在pH10的培养条件下分别保持了70%和43%的酶活性; 在50oC培养条件下EG和CBH分别保持了68%和59%的酶活性。表现出了耐热, 耐碱的特性。 相似文献
263.
同地共栖三种鼠耳蝠食性差异及其生态位分化 总被引:1,自引:0,他引:1
2005年9—11月在贵州省安龙县笃山乡暗河村,分析了共栖同一山洞3种鼠耳蝠的形态特征和食性。在体型上,华南水鼠耳蝠体重为(4.46±0.53)g,前臂长为(34.63±1.45)mm;毛腿鼠耳蝠体重为(5.15±1.76)g,前臂长为(35.20±1.07)mm;西南鼠耳蝠体重为(10.94±0.87)g,前臂长为(45.21±1.15)mm。3种鼠耳蝠的体重两两之间差异显著,西南鼠耳蝠与另外2种鼠耳蝠的前臂长相比两两之间差异显著。在食物组成上,华南水鼠耳蝠主要捕食近水面活动的双翅目及其幼虫,体积百分比和频次百分比分别为79.7%和100%;毛腿鼠耳蝠主要捕食双翅目和小型鞘翅目,体积百分比分别占59.6%和28.8%,频次百分比分别为91.3%和80.1%;西南鼠耳蝠的食物组成主要为近地面或在地表活动的鞘翅目步甲科和埋葬虫科昆虫,体积百分比和频次百分比分别为80.8%和100%;3种鼠耳蝠食物组成存在显著差异。结果表明,同地共栖3种鼠耳蝠除了形态结构上出现差异,食物组成也存在明显的差异。据此,推测3种鼠耳蝠可能采取不同的捕食生境和捕食策略,从而导致捕食生态位分离,避免出现激烈竞争,使得3种近缘鼠耳蝠能够同地共栖。 相似文献
264.
目的 通过研究HBME 1在恶性上皮性间皮瘤 (MEM )的表达及其与DNA含量的关系 ,探讨HBME 1在MEM与转移性卵巢腺癌 (OA)和转移性结直肠腺癌 (CA)的鉴别诊断中的意义。方法 采用免疫组化SP法染色 ,观察MEM、OA和CA组织标本中HBME 1的表达 ;同时利用图象分析仪对MEM的细胞DNA含量进行定量分析。结果 HBME 1的表达在MEM与CA两组间的差异有显著性 (P <0 0 1) ,在MEM与OA两组间的差异无显著性 (P <0 0 5 ) ,但着色方式不同 ;在MEM中 ,HBME 1表达阳性组与阴性组之间的DNA含量差异无显著性 (P <0 0 5 )。结论 HBME 1的表达与否与MEM的DNA含量无关 ,HBME 1可作为MEM与CA鉴别的阳性标记物的参考指标 ,在MEM与OA的鉴别中也有一定参考价值。 相似文献
265.
Min Lu Bin Liu Hui Xiong Fang Wu Chunhong Hu Ping Liu 《Journal of cellular and molecular medicine》2019,23(4):2431-2441
Despite initial dramatic efficacy of epidermal growth factor receptor (EGFR) tyrosine kinase inhibitors (EGFR‐TKIs) in EGFR‐mutant lung cancer patients, subsequent emergence of acquired resistance is almost inevitable. Resveratrol and its derivatives have been found to exert some effects on EGFR‐TKI resistance in non‐small cell lung cancer (NSCLC), but the underlying mechanisms remain unclear. We screened several NSCLC cell lines with gefitinib resistance by MTT assay and analysed the miR‐345/miR‐498 expression levels. NSCLC cells were pre‐treated with a resveratrol derivative, trans‐3,5,4‐trimethoxystilbene (TMS) and subsequently challenged with gefitinib treatment. The changes in apoptosis and miR‐345/miR‐498 expression were analysed by flow cytometry and q‐PCR respectively. The functions of miR‐345/miR‐498 were verified by CCK‐8 assay, cell cycle analysis, dual‐luciferase reporter gene assay and immunoblotting analysis. Our results showed that the expression of miR‐345 and miR‐498 significantly decreased in gefitinib resistant NSCLC cells. TMS pre‐treatment significantly upregulated the expression of miR‐345 and miR‐498 increasing the sensitivity of NSCLC cells to gefitinib and inducing apoptosis. MiR‐345 and miR‐498 were verified to inhibit proliferation by cell cycle arrest and regulate the MAPK/c‐Fos and AKT/Bcl‐2 signalling pathways by directly targeting MAPK1 and PIK3R1 respectively. The combination of TMS and gefitinib promoted apoptosis also by miR‐345 and miR‐498 targeting the MAPK/c‐Fos and AKT/Bcl‐2 signalling pathways. Our study demonstrated that TMS reduced gefitinib resistance in NSCLCs via suppression of the MAPK/Akt/Bcl‐2 pathway by upregulation of miR‐345/498. These findings would lay the theoretical basis for the future study of TMS for the treatment of EGFR‐TKI resistance in NSCLCs. 相似文献
266.
S K Srivastava S S Singhal X Hu Y C Awasthi P Zimniak S V Singh 《Archives of biochemistry and biophysics》1999,366(1):89-94
Alkylating agents are extensively used in the treatment of cancer. The clinical usefulness of this class of anticancer drugs, however, is often limited by the emergence of drug-resistant tumor cells. Increased glutathione (GSH) conjugation through catalysis by GSH S-transferases (GSTs) is believed to be an important mechanism in tumor cell resistance to alkylating agents. In the present study, we report that the allelic variants of human Pi class GST (hGSTP1-1), which differ in their primary structures at amino acids in positions 104 and/or 113, exhibit significant differences in their activity in the GSH conjugation of alkylating anticancer drug thiotepa. Mass spectrometry revealed that the major product of the reaction between thiotepa and GSH was the monoglutathionyl-thiotepa conjugate. While nonenzymatic formation of monoglutathionyl-thiotepa was negligible, the formation of this conjugate was increased significantly in the presence of hGSTP1-1 protein. The hGSTP1-1-catalyzed GSH conjugation of thiotepa was time and protein dependent and followed Michaelis-Menten kinetics. The catalytic efficiency of hGSTP1-1(I104, A113) variant was approximately 1.9- and 2.6-fold higher compared with hGSTP1-1(V104,A113) and hGSTP1-1(V104,V113) isoforms, respectively. The results of the present study indicate that the hGSTP1-1 polymorphism may be an important factor in GST-mediated tumor cell resistance to thiotepa, and that subjects homozygous for the hGSTP1-1(I104,A113) allele, which is most frequent in human populations, are likely to be at a greater risk for developing GST-mediated resistance to thiotepa than heterozygotes or homozygotes with valine 104 background. 相似文献
267.
268.
真菌种类多,分布广,对人类的生产和生活关系非常密切,因此近来对真菌特别是丝状真菌的研究日益引起高度重视。1981—1990年我们对桂北龙胜县里骆林区、桂中宜山县庆远林区、桂南岑溪县七坪林区和桂西田林县老山林区森林土壤微生物区系进行了分析,其中丝状真菌是我们重点研究的内容之一。在上述森林土壤中共分离出丝状真菌2084株,经鉴定归属于25属。现将广西森林土壤丝状真菌生态分布的研究结果报道如下。 相似文献
269.
自噬(autophagy)是一种溶酶体依赖性的细胞内降解途径,其主要功能是将生物大分子(蛋白质、多糖等)或细胞器(线粒体等)回收至溶酶体中并将其降解为单糖、氨基酸等小分子以重复利用。发现HOPS复合体中的两个基因vps39和vps41的缺失会导致酵母内GFP-ATG8大量积累。进一步研究表明,积累的原因是GFP-ATG8与液泡不能发生融合。而在HOPS复合体中的另外两个基因vps16和vps18缺失的情况下,自噬融合没有受到影响;在vps16和vps18双敲除的菌株中,自噬融合同样没有受到影响。该实验结果为理解HOPS复合体的功能和自噬体与液泡融合的过程提供了新的线索。 相似文献
270.
M. Calomme J. Hu K. Van Den Branden D. A. Vanden Berghe 《Biological trace element research》1995,47(1-3):379-383
Lactic acid bacteria are nonpathogenic bacteria commonly used in food processing. An evaluation was made of the capacity to
concentrate selenium in species of Lactobacillus. A selenium concentration of 1 μg/mL in the culture medium yielded in a bacterial
content of 400 μg/g dry biomass. Dialysis and TCA precipitation experiments of a native intracellular extract proved that
at least 80% of the total selenium is associated with organic molecules. Seleno-cysteine was identified as the only seleno-amino
acid present in the intracellular selenoproteins. This study shows that species of the lactic acid bacteria are able to concentrate
selenium intracellular as seleno-cysteine, which could be applied in supplementation studies. 相似文献