全文获取类型
收费全文 | 17970篇 |
免费 | 1499篇 |
国内免费 | 848篇 |
专业分类
20317篇 |
出版年
2023年 | 151篇 |
2022年 | 369篇 |
2021年 | 679篇 |
2020年 | 397篇 |
2019年 | 528篇 |
2018年 | 502篇 |
2017年 | 383篇 |
2016年 | 583篇 |
2015年 | 1053篇 |
2014年 | 1135篇 |
2013年 | 1321篇 |
2012年 | 1563篇 |
2011年 | 1390篇 |
2010年 | 851篇 |
2009年 | 735篇 |
2008年 | 997篇 |
2007年 | 817篇 |
2006年 | 838篇 |
2005年 | 674篇 |
2004年 | 599篇 |
2003年 | 480篇 |
2002年 | 408篇 |
2001年 | 400篇 |
2000年 | 325篇 |
1999年 | 272篇 |
1998年 | 198篇 |
1997年 | 164篇 |
1996年 | 180篇 |
1995年 | 154篇 |
1994年 | 154篇 |
1993年 | 125篇 |
1992年 | 202篇 |
1991年 | 168篇 |
1990年 | 138篇 |
1989年 | 164篇 |
1988年 | 98篇 |
1987年 | 97篇 |
1986年 | 89篇 |
1985年 | 109篇 |
1984年 | 56篇 |
1983年 | 58篇 |
1982年 | 77篇 |
1980年 | 46篇 |
1979年 | 62篇 |
1978年 | 41篇 |
1977年 | 52篇 |
1976年 | 42篇 |
1975年 | 49篇 |
1974年 | 45篇 |
1973年 | 46篇 |
排序方式: 共有10000条查询结果,搜索用时 15 毫秒
101.
Huanan Wen Jiaxin Zhong Bei Shen Tao Gan Chao Fu Zhihong Zhu Rui Li Xu Yang 《生物学前沿》2013,8(4):444-450
To compare the cytotoxicity on HeLa cells induced by nanosized and microsized tellurium powders, HeLa cells were exposed to different concentrations of tellurium powders (0, 50, 100, 150 and 200 μg/mL) for 12 h. In this study, detection of a series of biomarkers, including reactive oxygen species (ROS), glutathione (GSH), 8-hydroxy-2′-deoxyguanosine (8-OHdG), in addition to DNA and protein crosslink (DPC) and MTTassay, were conducted to evaluate the cytotoxicity. It is indicated that compared with the control group, there was no significant difference in the induced cytotoxicity at concentrations lower than 50 μg/mL for both nanosized and microsized tellurium powders. While there appears a significant difference in the induced cytotoxicity for nanosized tellurium powders when the concentration is higher than 100 μg/mL as well as for microsized tellurium powders when the concentration is higher than 200 μg/mL. Moreover, it is found that the cytotoxicity induced on HeLa cells exhibits a certain dose-effect relationship with the concentration of tellurium powders. A conclusion has been reached that the toxicity on HeLa cells can be induced by both nanosized and microsized tellurium powders, and the toxicity of the nanosized tellurium powders is significantly greater than the microsized one. 相似文献
102.
Measurements on the fluorescence induction of Triton X-100 extracted Photosystem II (PSII) particles confirmed the existence of the two sites of inhibition in PSII for the herbicide DCMU. The two sites were located on the reducing and oxidizing sides of PSII, respectively. The inhibition on the oxidizing side, unlike that on the reducing side which was of the "none or all" type, was found only to slow down the electron donation at low concentrations of DCMU. The results also suggested that the inhibitions of DCMU at these two sites were mutually exclusive, i.e., the binding on one site prevented the binding on the other site. 相似文献
103.
104.
105.
Here a highly sensitive electrochemical method is described for the detection of point mutation in DNA. Polymerization extension reaction is applied to specifically initiate enzymatic electrochemical amplification to improve the sensitivity and enhance the performance of point mutation detection. In this work, 5'-thiolated DNA probe sequences complementary to the wild target DNA are assembled on the gold electrode. In the presence of wild target DNA, the probe is extended by DNA polymerase over the free segment of target as the template. After washing with NaOH solution, the target DNA is removed while the elongated probe sequence remains on the sensing surface. Via hybridizing to the designed biotin-labeled detection probe, the extended sequence is capable of capturing detection probe. After introducing streptavidin-conjugated alkaline phosphatase (SA-ALP), the specific binding between streptavidin and biotin mediates a catalytic reaction of ascorbic acid 2-phosphate (AA-P) substrate to produce a reducing agent ascorbic acid (AA). Then the silver ions in solution are reduced by AA, leading to the deposition of silver metal onto the electrode surface. The amount of deposited silver which is determined by the amount of wild target can be quantified by the linear sweep voltammetry (LSV). The present approach proved to be capable of detecting the wild target DNA down to a detection limit of 1.0×10(-14) M in a wide target concentration range and identifying -28 site (A to G) of the β-thalassemia gene, demonstrating that this scheme offers a highly sensitive and specific approach for point mutation detection. 相似文献
106.
107.
108.
Zhang Hao Qing Zhao Xue Qiang Chen Yi Ling Zhang Ling Yu Shen Ren Fang 《Plant and Soil》2019,439(1-2):293-308
Plant and Soil - Biocrusts are important functional units in dryland ecosystems. Regarded as ecosystem engineers, cyanobacteria in biocrusts contribute several major physico-chemical and biological... 相似文献
109.
110.
Ruizhe Shen Shengjian Qi Shidan Cheng 《Biochemical and biophysical research communications》2010,394(4):1047-38
High levels of SOX4 expression have been found in a variety of human cancers, such as lung, brain and breast cancers. However, the expression of SOX4 in gastric tissues remains unknown. The SOX4 expression was detected using immunohistochemical staining and semi-quantitative RT-PCR, and our results showed that SOX4 was up-regulated in gastric cancer compared to benign gastric tissues. To further elucidate the molecular mechanisms underlying up-regulation of SOX4 in gastric cancers, we analyzed the expression of microRNA-129-2 (miR-129-2) gene, the epigenetic repression of which leads to overexpression of SOX4 in endometrial cancer. We found that up-regulation of SOX4 was inversely associated with the epigenetic silencing of miR-129-2 in gastric cancer, and restoration of miR-129-2 down-regulated SOX4 expression. We also found that inactivation of SOX4 by siRNA and restoration of miR-129-2 induced apoptosis in gastric cancer cells. 相似文献