全文获取类型
收费全文 | 224篇 |
免费 | 27篇 |
出版年
2019年 | 2篇 |
2017年 | 5篇 |
2016年 | 10篇 |
2015年 | 14篇 |
2014年 | 17篇 |
2013年 | 11篇 |
2012年 | 14篇 |
2011年 | 9篇 |
2010年 | 7篇 |
2009年 | 9篇 |
2008年 | 5篇 |
2007年 | 4篇 |
2006年 | 10篇 |
2005年 | 10篇 |
2004年 | 4篇 |
2003年 | 3篇 |
2002年 | 3篇 |
2001年 | 8篇 |
2000年 | 9篇 |
1999年 | 8篇 |
1998年 | 7篇 |
1997年 | 3篇 |
1996年 | 10篇 |
1995年 | 3篇 |
1994年 | 2篇 |
1992年 | 2篇 |
1990年 | 2篇 |
1988年 | 3篇 |
1987年 | 5篇 |
1981年 | 2篇 |
1980年 | 4篇 |
1979年 | 3篇 |
1978年 | 2篇 |
1977年 | 3篇 |
1974年 | 3篇 |
1973年 | 2篇 |
1965年 | 3篇 |
1961年 | 2篇 |
1960年 | 2篇 |
1940年 | 2篇 |
1939年 | 1篇 |
1937年 | 1篇 |
1935年 | 3篇 |
1934年 | 2篇 |
1931年 | 1篇 |
1930年 | 3篇 |
1929年 | 1篇 |
1928年 | 1篇 |
1922年 | 1篇 |
1914年 | 1篇 |
排序方式: 共有251条查询结果,搜索用时 15 毫秒
61.
Phylogenetic screening of the human genome: identification of differentially hybridizing repetitive sequence families 总被引:1,自引:0,他引:1
The phi-screen, a method of phylogenetic screening, can be employed to
detect repetitive sequence families that differentially hybridize between
closely related species. Such differences may involve sequence divergence
or variations in copy number, including total presence versus absence of a
family of repeated DNA. We present the results of a phi-screen comparing
the human genome to that of the prosimian, Galago crassicaudatus. Three
human repetitive families that are divergent or not present in galago have
been detected. One of these families is described in detail; it is similar
among the anthropoids but is present in a lower copy number and/or
divergent form in prosimians. The family is clearly related to the
transposon-like human element (THE) described by Paulson et al. (1985).
THEs have long terminal repeats reminiscent of retroviruses but are unique
in that they have no sequence similarity to known mammalian retroviruses.
The sequence of a solo long terminal repeat, found unassociated with THE
internal sequence, is presented. This family member, THE p2, is bordered by
a 5-bp target-site repeat and is interrupted by the insertion of an Alu
element. A solo THE element sequenced by Wiginton et al. (1986) contains an
insertion of Alu at precisely the same position as does THE p2.
相似文献
62.
The alcohol dehydrogenase (Adh) region from five planitibia subgroup
species of Hawaiian picture-wing Drosophila has been cloned. A total of 15
kb of DNA in and around the Adh gene has been compared among the five
species. Genetic distances were calculated to determine evolutionary
relationships. These distances agree with previous distances determined by
protein polymorphism and DNA hybridization techniques and can be
interpreted in terms of specific island colonization and speciation
(founder) events over the past 5 Myr. Examination of the restriction maps
of the cloned Adh region from the five species shows many instances of
small deletions, insertion of a transposable element in D. heteroneura, and
the existence of a highly variable region on the 3' side of the Adh gene.
Clustering relationships and rates of DNA change are calculated and
compared with the relationship found for other species of Drosophila.
相似文献
63.
Site-directed mutagenesis was used to generate three mutations in the uncB gene encoding the a-subunit of the F0 portion of the F0F1-ATPase of Escherichia coli. These mutations directed the substitution of Arg-210 by Gln, or of His-245 by Leu, or of both Lys-167 and Lys-169 by Gln. The mutations were incorporated into plasmids carrying all the structural genes encoding the F0F1-ATPase complex and these plasmids were used to transform strain AN727 (uncB402). Strains carrying either the Arg-210 or His-245 substitutions were unable to grow on succinate as sole carbon source and had uncoupled growth yields. The substitution of Lys-167 and Lys-169 by Gln resulted in a strain with growth characteristics indistinguishable from a normal strain. The properties of the membranes from the Arg-210 or His-245 mutants were essentially identical, both being proton impermeable and both having ATPase activities resistant to the inhibitor DCCD. Furthermore, in both mutants, the F1-ATPase activities were inhibited by about 50% when bound to the membranes. The membrane activities of the mutant with the double lysine change were the same as for a normal strain. The results are discussed in relation to a previously proposed model for the F0 (Cox, G.B., Fimmel, A.L., Gibson, F. and Hatch, L. (1986) Biochim. Biophys. Acta 849, 62–69). 相似文献
64.
65.
W. Howitt Hastings 《BMJ (Clinical research ed.)》1930,2(3636):448-449
66.
67.
68.
69.
70.
Wagner Vital Gustavo Lazzaro Rezende Leonardo Abreu Jorge Moraes Francisco JA Lemos Itabajara da Silva VazJr Carlos Logullo 《BMC developmental biology》2010,10(1):25