全文获取类型
收费全文 | 75篇 |
免费 | 11篇 |
出版年
2021年 | 1篇 |
2019年 | 1篇 |
2018年 | 1篇 |
2017年 | 1篇 |
2016年 | 4篇 |
2015年 | 7篇 |
2014年 | 4篇 |
2011年 | 3篇 |
2010年 | 4篇 |
2009年 | 6篇 |
2008年 | 2篇 |
2007年 | 8篇 |
2006年 | 4篇 |
2005年 | 4篇 |
2004年 | 4篇 |
2003年 | 4篇 |
2001年 | 1篇 |
2000年 | 3篇 |
1998年 | 5篇 |
1997年 | 1篇 |
1996年 | 1篇 |
1995年 | 2篇 |
1994年 | 1篇 |
1992年 | 1篇 |
1990年 | 1篇 |
1989年 | 1篇 |
1988年 | 1篇 |
1987年 | 1篇 |
1986年 | 1篇 |
1981年 | 3篇 |
1979年 | 1篇 |
1977年 | 2篇 |
1976年 | 1篇 |
1968年 | 1篇 |
排序方式: 共有86条查询结果,搜索用时 78 毫秒
81.
Sacha?AFT?van Hijum Anne?de Jong Richard?JS?Baerends Harma?A?Karsens Naomi?E?Kramer Rasmus?Larsen Chris?D?den Hengst Casper?J?Albers Jan?Kok Oscar?P?KuipersEmail author 《Genome biology》2005,6(4):P4
Background
In research laboratories using DNA-microarrays, usually a number of researchers perform experiments, each generating possible sources of error. There is a need for a quick and robust method to assess data quality and sources of errors in DNA-microarray experiments. To this end, a novel and cost-effective validation scheme was devised, implemented, and employed. 相似文献82.
Gwaltney SL O'Connor SJ Nelson LT Sullivan GM Imade H Wang W Hasvold L Li Q Cohen J Gu WZ Tahir SK Bauch J Marsh K Ng SC Frost DJ Zhang H Muchmore S Jakob CG Stoll V Hutchins C Rosenberg SH Sham HL 《Bioorganic & medicinal chemistry letters》2003,13(7):1359-1362
Inhibitors of farnesyltransferase are effective against a variety of tumors in mouse models of cancer. Clinical trials to evaluate these agents in humans are ongoing. In our effort to develop new farnesyltransferase inhibitors, we have discovered a series of aryl tetrahydropyridines that incorporate substituted glycine, phenylalanine and histidine residues. The design, synthesis, SAR and biological properties of these compounds will be discussed. 相似文献
83.
Cell membranes are capable of withstanding significant osmotic stress, the exact amount of which varies with the lipid composition. In this paper, we examine the effect that salt concentration has on the lysis pressure of membranes containing anionic lipids. Vesicles containing varying amounts of phosphatidylcholine and phosphatidylglycerol were osmotically stressed using NaCl as the osmolyte. The lysis pressure was observed to vary linearly with the Debye screening length and the extent of the variation was linear with anionic lipid content. The implications these results have for cells that frequently encounter low solute environments are discussed. 相似文献
84.
Liane FM Finotelo Paulo JS Amaral Julio C Pieczarka Edivaldo HC de Oliveira Alcides Pissinati Michaela Neusser Stephan Müller Cleusa Y Nagamachi 《BMC evolutionary biology》2010,10(1):189
Background
The New World monkey (Platyrrhini) subfamily Pitheciinae is represented by the genera Pithecia, Chiropotes and Cacajao. In this work we studied the karyotypes of Pithecia irrorata (2n = 48) and Cacajao calvus rubicundus (2n = 45 in males and 2n = 46 in females) by G- and C-banding, NOR staining and chromosome painting using human and Saguinus oedipus whole chromosome probes. The karyotypes of both species were compared with each other and with Chiropotes utahicki (2n = 54) from the literature. 相似文献85.
Shechinah Felice Choragudi Ganesh Kumar Veeramachaneni BV Raman Bondili JS 《Bioinformation》2014,10(8):507-511
Endo- β-N-acetylgucosaminidases (ENGases) are the enzymes that catalyze both hydrolysis and
transglycosylation reactions. It is of interest to study ENGases because of their ability to synthesize glycopeptides.
Homology models of Human, Arabidopsis thaliana and Sorghum ENGases were developed and their active sites
marked based on information available from Arthrobacter protophormiae (PDB ID: 3FHQ) ENGase. Further, these
models were docked with the natural substrate GlcNAc-Asn and the inhibitor Man3GlcNAc-thiazoline. The catalytic
triad of Asn, Glu and Tyr (N171, E173 and Y205 of bacteria) were found to be conserved across the phyla. The crucial
Y299F mutation showing 3 times higher transglycosylation activity than in wild type Endo-A is known. The hydrolytic
activity remained unchanged in bacteria, while the transglycosylation activity increased. This Y to F change is found
to be naturally evolved and should be attributing higher transglycosylation rates in human and Arabidopsis thaliana
ENGases. Ligand interactions Ligplots revealed the interaction of amino acids with hydrophobic side chains and polar
uncharged side chain amino acids. Thus, structure based molecular model-ligand interactions provide insights into
the catalytic mechanism of ENGases and assist in the rational engineering of ENGases. 相似文献
86.