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11.
Davoud Nouri Inanlou Bagher Yakhchali Hossein Khanahmad Mossa Gardaneh Hesam Movassagh Reza Ahangari Cohan Mehdi Shafiee Ardestani Reza Mahdian Sirous Zeinali 《Biotechnology letters》2010,32(11):1615-1621
We have developed an integrase-defective lentiviral (LV) vector in combination with a gene-targeting approach for gene therapy
of β-thalassemia. The β-globin gene-targeting construct has two homologous stems including sequence upstream and downstream
of the β-globin gene, a β-globin gene positioned between hygromycin and neomycin resistant genes and a herpes simplex virus
type 1 thymidine kinase (HSVtk) suicide gene. Utilization of integrase-defective LV as a vector for the β-globin gene increased the number of selected clones
relative to non-viral methods. This method represents an important step toward the ultimate goal of a clinical gene therapy
for β-thalassemia. 相似文献
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Amir Ahmad Akhavan Ali Khamesipour Yavar Rassi Shaden Kamhawi Mohammad Hossein Arandian Niloufar Jalali-zand Niloufar Shareghi Mohammad Reza Yaghoobi-Ershadi 《Experimental parasitology》2010,126(4):552-556
Many rodent species act as reservoir hosts of zoonotic cutaneous leishmaniasis in endemic areas. In the present study a simple and reliable assay based on nested PCR was developed for the detection and identification of Leishmania parasites from rodent skin samples. We designed Leishmania-specific primers that successfully amplified ITS regions of Leishmania major, Leishmania gerbilli and Leishmania turanica using nested PCR. Out of 95 field collected Rhombomys opimus, 21 were positive by microscopic examination and 48 by nested PCR. The percentage of gerbils infected with L. major, L. gerbilli and L. turanica was 3.2%, 1.1% and 27.4%, respectively. In 15.8% of the rodents, we found mixed natural infections by L. major and L. turanica, 1.1% by L. major and L. gerbilli, and 2.1% by the three species. We concluded that this method is simple and reliable for detecting and identifying Leishmania species circulating in rodent populations. 相似文献
13.
Soni Pullamsetti Stefanie Krick Hüseyin Yilmaz Hossein Ardeschir Ghofrani Christian Schudt Norbert Weissmann Beate Fuchs Werner Seeger Friedrich Grimminger Ralph Theo Schermuly 《Respiratory research》2005,6(1):128
Background
The aim of the study was to assess the chronic effects of combined phosphodiesterase 3/4 inhibitor tolafentrine, administered by inhalation, during monocrotaline-induced pulmonary arterial hypertension (PAH) in rats.Methods
CD rats were given a single subcutaneous injection of monocrotaline to induce PAH. Four weeks after, rats were subjected to inhalation of tolafentrine or sham nebulization in an unrestrained, whole body aerosol exposure system. In these animals (i) the acute pulmonary vasodilatory efficacy of inhaled tolafentrine (ii) the anti-remodeling effect of long-term inhalation of tolafentrine (iii) the effects of tolafentrine on the expression profile of 96 genes encoding cell adhesion and extracellular matrix regulation were examined. In addition, the inhibitory effect of tolafentrine on ex vivo isolated pulmonary artery SMC cell migration was also investigated.Results
Monocrotaline injection provoked severe PAH (right ventricular systolic pressure increased from 25.9 ± 4.0 to 68.9 ± 3.2 after 4 weeks and 74.9 ± 5.1 mmHg after 6 weeks), cardiac output depression and right heart hypertrophy. The media thickness of the pulmonary arteries and the proportion of muscularization of small precapillary resistance vessels increased dramatically, and the migratory response of ex-vivo isolated pulmonary artery smooth muscle cells (PASMC) was increased. Micro-arrays and subsequent confirmation with real time PCR demonstrated upregulation of several extracellular matrix regulation and adhesion genes, such as matrixmetalloproteases (MMP) 2, 8, 9, 10, 11, 12, 20, Icam, Itgax, Plat and serpinb2. When chronically nebulized from day 28 to 42 (12 daily aerosol maneuvers), after full establishment of severe pulmonary hypertension, tolafentrine reversed about 60% of all hemodynamic abnormalities, right heart hypertrophy and monocrotaline-induced structural lung vascular changes, including the proportion of pulmonary artery muscularization. The upregulation of extracellular matrix regulation and adhesion genes was reduced by nearly 80% by inhalation of the tolafentrine. When assessed in vitro, tolafentrine blocked the enhanced PASMC migratory response.Conclusion
In conclusion, we demonstrate for the first time that inhalation of combined PDE3/4 inhibitor reverses pulmonary hypertension fully developed in response to monocrotaline in rats. This "reverse-remodeling" effect includes structural changes in the lung vascular wall and key molecular pathways of matrix regulation, concomitant with 60% normalization of hemodynamics. 相似文献14.
In vitro improvement of quail primordial germ cell expansion through activation of TGF‐beta signaling pathway 下载免费PDF全文
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16.
Foroughi Kobra Jahanbani Sarvin Nazarnezhad Simin Khastar Hossein Jafarisani Moslem Tashakori Mersedeh Kazemi Seyedeh Sareh 《International journal of peptide research and therapeutics》2020,26(2):1115-1126
International Journal of Peptide Research and Therapeutics - Survivin is a unique member of the inhibitor of apoptosis protein family. Research has approved Survivin’s ability to interact... 相似文献
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Mojtaba Tabatabaei Yazdi Younes Ghasemi Abdollah Ghasemian Shadman Shokravi Hossein Niknahad Mohsen Amini Ali Dehshahri Mohammad Ali Faramarzi 《World journal of microbiology & biotechnology》2005,21(6-7):811-814
Summary The bioconversion of hydrocortisone by a locally isolated strain of cyanobacterium Fischerella ambigua PTCC 1635 was investigated. Fischerella ambigua had not been previously examined for this potential. The fermentation led to production of 11β,17α, 20β, 21-tetrahydroxypregn-4-en-3-one and 11β-hydroxyandrost-4-en-3,17-dione. The metabolites were isolated and purified by chromatographic methods and identified using
instrumental analyses. 相似文献
19.
Mahdi Yahyazadeh Reza Omidbaigi Rasoul Zare Hossein Taheri 《World journal of microbiology & biotechnology》2008,24(8):1445-1450
The antifungal action of four essential oils of Foeniculum vulgare (fennel), Thymus vulgaris (thyme), Eugenia caryophyllata (Clove) and Salvia officinalis (sage) was tested in vitro against Penicillium digitatum Sacc. Direct contact and vapour phase were used to test the antifungal activity of these essential oils against P. digitatum that is responsible for green mould rot of citrus fruits. The vapour phase and direct contact of clove and thyme essential
oils exhibited the strongest toxicity and totally inhibited the mycelial growth of the test fungus. Thyme and clove essential
oils completely inhibited P. digitatum growth either when added into the medium 600 μl l−1 or by their volatiles with 24 μl per 8 cm diameter Petri dish. In in vitro mycelial growth assay showed fungistatic and fungicidal
activity by clove and thyme essential oils. Sage and fennel oils did not show any inhibitory activity on this fungus. Scanning
electron microscopy (SEM) was done to study the mode of action of clove oil in P. digitatum and it was observed that treatment with the oil leads to large alterations in hyphal morphology. 相似文献
20.
Negin Parsamanesh Maryam Moossavi Tahmineh Tavakkoli Hossein Javdani Tahereh Fakharian Seyedeh Zahra Moossavi Mohsen Naseri 《Journal of cellular physiology》2019,234(9):15044-15047
Gastric cancer is the second cause of cancer-related mortality and the fourth most common cancers worldwide. Owing to the immune modulatory effect of vitamin D in the body, the role of vitamin D receptor gene in vitamin D regulation receives a great deal of research interest. The aim of the current study was to highlight the association between two variants of TaqI and FokI in the vitamin D receptor gene and gastric cancer predisposition in a sample of South Khorasan population. The present investigation consisted of 69 patients affected with gastric cancer and 100 healthy individuals. The genomic DNA was extracted by salting out the protocol from peripheral venous blood. Genotyping of TaqI and FokI variants were performed by PCR-RFLP method. Our findings manifested that TC genotype of TaqI polymorphism was statistically significant between the case and the control groups (p = 0.002). Moreover, the frequency of TC + CC genotypes was statistically significant between the two groups (p = 0.009). Furthermore, we could not find any meaningful association between FokI variant and the participant groups. The present results declared that, in our population, TC genotype of TaqI polymorphism has an association with gastric cancer susceptibility. In addition, more investigation with greater sample sizes is needed to confirm our results. 相似文献