全文获取类型
收费全文 | 2541篇 |
免费 | 168篇 |
国内免费 | 1篇 |
出版年
2021年 | 20篇 |
2018年 | 21篇 |
2017年 | 19篇 |
2016年 | 31篇 |
2015年 | 46篇 |
2014年 | 57篇 |
2013年 | 89篇 |
2012年 | 105篇 |
2011年 | 112篇 |
2010年 | 77篇 |
2009年 | 66篇 |
2008年 | 110篇 |
2007年 | 113篇 |
2006年 | 123篇 |
2005年 | 116篇 |
2004年 | 109篇 |
2003年 | 91篇 |
2002年 | 104篇 |
2001年 | 50篇 |
2000年 | 39篇 |
1999年 | 56篇 |
1998年 | 40篇 |
1997年 | 44篇 |
1996年 | 30篇 |
1995年 | 37篇 |
1994年 | 49篇 |
1993年 | 45篇 |
1992年 | 45篇 |
1991年 | 48篇 |
1990年 | 51篇 |
1989年 | 40篇 |
1988年 | 35篇 |
1987年 | 36篇 |
1986年 | 38篇 |
1985年 | 40篇 |
1984年 | 29篇 |
1983年 | 36篇 |
1982年 | 39篇 |
1981年 | 36篇 |
1980年 | 32篇 |
1979年 | 40篇 |
1978年 | 24篇 |
1977年 | 18篇 |
1976年 | 19篇 |
1975年 | 22篇 |
1974年 | 15篇 |
1973年 | 23篇 |
1972年 | 18篇 |
1970年 | 14篇 |
1965年 | 14篇 |
排序方式: 共有2710条查询结果,搜索用时 31 毫秒
151.
152.
Sofía Ruiz-Cruz Andrea Erazo Garzon Philip Kelleher Francesca Bottacini Solvej Østergaard Breum Horst Neve Knut J. Heller Finn K. Vogensen Simon Palussière Pascal Courtin Marie-Pierre Chapot-Chartier Evgeny Vinogradov Irina Sadovskaya Jennifer Mahony Douwe van Sinderen 《Microbial biotechnology》2022,15(12):2875-2889
The first step in phage infection is the recognition of, and adsorption to, a receptor located on the host cell surface. This reversible host adsorption step is commonly followed by an irreversible event, which involves phage DNA delivery or release into the bacterial cytoplasm. The molecular components that trigger this latter event are unknown for most phages of Gram-positive bacteria. In the current study, we present a comparative genome analysis of three mutants of Lactococcus cremoris 3107, which are resistant to the P335 group phage TP901-1 due to mutations that affect TP901-1 DNA release. Through genetic complementation and phage infection assays, a predicted lactococcal three-component glycosylation system (TGS) was shown to be required for TP901-1 infection. Major cell wall saccharidic components were analysed, but no differences were found. However, heterologous gene expression experiments indicate that this TGS is involved in the glucosylation of a cell envelope-associated component that triggers TP901-1 DNA release. To date, a saccharide modification has not been implicated in the DNA delivery process of a Gram-positive infecting phage. 相似文献
153.
154.
155.
Hashemy SI Johansson C Berndt C Lillig CH Holmgren A 《The Journal of biological chemistry》2007,282(19):14428-14436
Glutathione (GSH) is the major intracellular thiol present in 1-10-mm concentrations in human cells. However, the redox potential of the 2GSH/GSSG (glutathione disulfide) couple in cells varies in association with proliferation, differentiation, or apoptosis from -260 mV to -200 or -170 mV. Hydrogen peroxide is transiently produced as second messenger in receptor-mediated growth factor signaling. To understand oxidation mechanisms by GSSG or nitric oxide-related nitrosylation we studied effects on glutaredoxins (Grx), which catalyze GSH-dependent thiol-disulfide redox reactions, particularly reversible glutathionylation of protein sulfhydryl groups. Human Grx1 and Grx2 contain Cys-Pro-Tyr-Cys and Cys-Ser-Tyr-Cys active sites and have three and two additional structural Cys residues, respectively. We analyzed the redox state and disulfide pairing of Cys residues upon GSSG oxidation and S-nitrosylation. Cytosolic/nuclear Grx1 was partly inactivated by both S-nitrosylation and oxidation. Inhibition by nitrosylation was reversible under anaerobic conditions; aerobically it was stronger and irreversible, indicating inactivation by nitration. Oxidation of Grx1 induced a complex pattern of disulfide-bonded dimers and oligomers formed between Cys-8 and either Cys-79 or Cys-83. In addition, an intramolecular disulfide between Cys-79 and Cys-83 was identified, predicted to have a profound effect on the three-dimensional structure. In contrast, mitochondrial Grx2 retains activity upon oxidation, did not form disulfide-bonded dimers or oligomers, and could not be S-nitrosylated. The dimeric iron sulfur cluster-coordinating inactive form of Grx2 dissociated upon nitrosylation, leading to activation of the protein. The striking differences between Grx1 and Grx2 reflect their diverse regulatory functions in vivo and also adaptation to different subcellular localization. 相似文献
156.
157.
Volk A Karbasiyan M Semmler A Todt U Urbach H Klockgether T Linnebank M 《Birth defects research. Part A, Clinical and molecular teratology》2007,79(3):249-251
BACKGROUND: The symptom triad of autosomal dominant Currarino syndrome (CS; MIM #176450) consists of anorectal malformation, a sacral bone defect, and presacral masses. Mutations in the homeoboxHLXB9 gene have already been described in a subset of sacrococcygeal anomalies characterized by partial sacral agenesis. CASE: We report a 28-year-old male patient with Currarino syndrome due to a heterozygous novel frame-shift mutation c.336dupG (p.P113fsX224) in the homeoboxHLXB9 gene. CONCLUSIONS: Molecular diagnostics may be helpful in cases of Hirschsprung's disease accompanied by other symptoms suggestive for Currarino syndrome, since it can lead to major complications such as perianal sepsis, meningitis, and malignant transformation. 相似文献
158.
Ingrid van der Pluijm George A Garinis Renata M. C Brandt Theo G. M. F Gorgels Susan W Wijnhoven Karin E. M Diderich Jan de Wit James R Mitchell Conny van Oostrom Rudolf Beems Laura J Niedernhofer Susana Velasco Errol C Friedberg Kiyoji Tanaka Harry van Steeg Jan H. J Hoeijmakers Gijsbertus T. J van der Horst 《PLoS biology》2007,5(1)
159.
The redox-switch domain of Hsp33 functions as dual stress sensor 总被引:1,自引:0,他引:1
Ilbert M Horst J Ahrens S Winter J Graf PC Lilie H Jakob U 《Nature structural & molecular biology》2007,14(6):556-563
The redox-regulated chaperone Hsp33 is specifically activated upon exposure of cells to peroxide stress at elevated temperatures. Here we show that Hsp33 harbors two interdependent stress-sensing regions located in the C-terminal redox-switch domain of Hsp33: a zinc center sensing peroxide stress conditions and an adjacent linker region responding to unfolding conditions. Neither of these sensors works sufficiently in the absence of the other, making the simultaneous presence of both stress conditions a necessary requirement for Hsp33's full activation. Upon activation, Hsp33's redox-switch domain adopts a natively unfolded conformation, thereby exposing hydrophobic surfaces in its N-terminal substrate-binding domain. The specific activation of Hsp33 by the oxidative unfolding of its redox-switch domain makes this chaperone optimally suited to quickly respond to oxidative stress conditions that lead to protein unfolding. 相似文献
160.