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OI Klychnikov AV Drabkin OV Vasilenko YS Pavlov MS Trofimova IN Smolenskaya AA Rozenkranz AS Sobolev AV Babakov 《Biochemistry. Biokhimii?a》1998,63(9):1083-1089
Higher plant plasma membranes carry receptors of different affinity for the phytotoxin fusicoccin. Reception of fusicoccin involves proteins belonging to the highly conserved 14-3-3 family, but the complete structure of the fusicoccin receptor (FCR) is unknown. Using radiation inactivation analysis, we estimated the molecular masses of low-affinity and high-affinity FCR at 63 +/- 7 and 130 +/- 15 kD, respectively. The dose dependences of receptor inactivation indicate that microsomal specimens contain "silent" FCRs of 420 +/- 90 kD in amounts commensurate with that of the active FCRs. Both low- and high-affinity FCRs are inactivated by hydrolytic enzymes from the outer surface of the plasma membrane, and impairment of protoplast integrity causes an irreversible transition of the low-affinity binding site into the high-affinity one. A scheme is proposed for the organization of different types of FCR in the plasma membrane, implying that the membrane affinity for fusicoccin reflects the interaction between proteins in the FCR complex. 相似文献
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OV Salata 《Journal of nanobiotechnology》2007,5(1):5-2
Nanotechnology in Therapeutics: Current Technology and Applications, Edited by Nicholas A. Peppas, J. Zach Hilt and J. Brock
Thomas (Horizon Bioscience, 2007) contains seventeen chapters written by leading specialists in the field of polymeric materials
for drug delivery and holds wealth of background as well as state of the art material divided into four sections: "Intelligent
Therapeutics and Responsive Delivery Systems for Improved Absorption and Delivery", "Therapeutic Micro- and Nanodevices",
"Nanostructured Therapeutic Materials" and "Nanoparticulate Systems in Intelligent Therapy". This newly published volume provides
a stimulating read and a good point of reference to researchers wishing to explore the interdisciplinary fusion of nnanotechnology
and medical therapeutics. The following gives brief summary and critically reviews the book. 相似文献
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RENATA SZYMAŃSKA JERZY KRUK MICHAELA SEDLÁŘOVÁ PAVEL POSPÍŠIL 《Plant, cell & environment》2014,37(2):392-401
In the present study, singlet oxygen (1O2) scavenging activity of tocopherol and plastochromanol was examined in tocopherol cyclase‐deficient mutant (vte1) of Arabidopsis thaliana lacking both tocopherol and plastochromanol. It is demonstrated here that suppression of tocopherol and plastochromanol synthesis in chloroplasts isolated from vte1 Arabidopsis plants enhanced 1O2 formation under high light illumination as monitored by electron paramagnetic resonance spin‐trapping spectroscopy. The exposure of vte1 Arabidopsis plants to high light resulted in the formation of secondary lipid peroxidation product malondialdehyde as determined by high‐pressure liquid chromatography. Furthermore, it is shown here that the imaging of ultra‐weak photon emission known to reflect oxidation of lipids was unambiguously higher in vte1 Arabidopsis plants. Our results indicate that tocopherol and plastochromanol act as efficient 1O2 scavengers and protect effectively lipids against photooxidative damage in Arabidopsis plants. 相似文献