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101.
The catchment is the smallest natural unit of the landscape that combines linked terrestrial and aquatic ecosystems. The fluxes of water and elements through the catchment link the various components of the system; biotic and abiotic, terrestrial and aquatic, plants and soils, atmosphere and vegetation, soils and waters. A large amount of empirical evidence now demonstrates that the impacts of natural and anthropogenic changes on the various components of catchment ecosystems are tightly connected. 相似文献
102.
J. L. Chan L. Saénz C. Talavera R. Hornung M. Robert C. Oropeza 《Plant cell reports》1998,17(6-7):515-521
A protocol was developed for coconut regeneration using plumules from mature zygotic embryos as explants, and media with
the synthetic growth regulators 2,4-dichlorophenoxyacetic acid and 6-benzylaminopurine. Evidence for the regeneration process
from these tissues occurring through somatic embryogenesis is presented. The somatic embryos were capable of germination,
subsequent development into plantlets and successful transfer to the nursery. The yields were larger, nearly twofold for calli
and over tenfold for calli bearing somatic embryos, than those previously reported with inflorescence explants. The present
protocol thus represents an improvement in time and yield over previous protocols. Even though plumule explants are not the
ideal tissue source due to possible genetic heterogeneity, the improvements made here may be applicable to tissues from mature
plants. In addition, micropropagation of coconut using plumules is potentially useful when they are obtained from fruit produced
from selected parents of outstanding performance, such as those resistant to diseases.
Received: 19 March 1997 /Revision received: 11 September 1997 / Accepted: 4 October 1997 相似文献
103.
Epithelial-mesenchymal interactions control basement membrane production and differentiation in cultured and transplanted mouse keratinocytes 总被引:10,自引:0,他引:10
Axel Bohnert Jürgen Hornung Ian C. Mackenzie Norbert E. Fusenig 《Cell and tissue research》1986,244(2):413-429
Summary The effects of mesenchyme and substratum on epidermal differentiation and formation of a basement membrane (BM) were analyzed in vitro and in vivo. Primary epidermal cell cultures (PEC) from neonatal mice were grown: (1) on plastic culture dishes; (2) on lifted collagen gels, either alone or (3) in recombination with mesenchyme; (4) after reimplantation in vivo either directly on mesenchyme or (5) on collagen interposed between keratinocytes and mesenchyme. Differentiation of the epithelium and formation of a BM were examined by electron microscopy, and expression of BM constituents (type IV collagen, laminin, fibronectin, bullous pemphigoid antigen, and heparan sulfate proteoglycan) by indirect immunofluorescence. PEC on plastic or on collagen gels showed poor differentiation, a structured BM was not visible, and the expression and deposition of BM constituents was incomplete. Upon reimplantation in vivo, differentiation was normalized, expression of BM components complete and a structured BM reformed. This effect does not depend on immediate contact of epidermal cells with mesenchyme. When PEC on collagen gel were similarly associated with dermal mesenchyme in vitro, epidermal differentiation and expression of BM components were almost normalized, but a structured BM was absent. These findings demonstrate that formation of the BM in epidermis is a function of keratinocytes and, like differentiation is subject to mesenchymal control. A structural BM is not a prerequisite but rather an additional criterion of normal epidermal differentiation.
Send offprint requests to: Norbert E. Fusenig, M.D., Division of Differentiation and Carcinogenesis in vitro, Institute of Biochemistry, German Cancer Research Center (DKFZ), Im Neuenheimer Feld 280, D-6900 Heidelberg, Federal Republic of GermanyPart of the work was performed when I.C. Mackenzie was guest scientist at the DKFZSupported by the Deutsche Forschungsgemeinschaft (Fu 91/2) 相似文献
104.
Mice lacking DNase II display a polyarthritis-like disease phenotype that is driven by translocation of self-DNA into the cytoplasm of phagocytic cells, where it is sensed by pattern recognition receptors. While pro-inflammatory gene expression is non-redundantly linked to the presence of STING in these mice, the contribution of the inflammasome pathway has not been explored. To this end, we studied the role of the DNA-sensing inflammasome receptor AIM2 in this self-DNA driven disease model. Arthritis-prone mice lacking AIM2 displayed strongly decreased signs of joint inflammation and associated histopathological findings. This was paralleled with a reduction of caspase-1 activation and pro-inflammatory cytokine production in diseased joints. Interestingly, systemic signs of inflammation that are associated with the lack of DNase II were not dependent on AIM2. Taken together, these data suggest a tissue-specific role for the AIM2 inflammasome as a sensor for endogenous DNA species in the course of a ligand-dependent autoinflammatory condition. 相似文献
105.
Boehlke C Kotsis F Patel V Braeg S Voelker H Bredt S Beyer T Janusch H Hamann C Gödel M Müller K Herbst M Hornung M Doerken M Köttgen M Nitschke R Igarashi P Walz G Kuehn EW 《Nature cell biology》2010,12(11):1115-1122
The mTOR pathway is the central regulator of cell size. External signals from growth factors and nutrients converge on the mTORC1 multi-protein complex to modulate downstream targets, but how the different inputs are integrated and translated into specific cellular responses is incompletely understood. Deregulation of the mTOR pathway occurs in polycystic kidney disease (PKD), where cilia (filiform sensory organelles) fail to sense urine flow because of inherited mutations in ciliary proteins. We therefore investigated if cilia have a role in mTOR regulation. Here, we show that ablation of cilia in transgenic mice results in enlarged cells when compared with control animals. In vitro analysis demonstrated that bending of the cilia by flow is required for mTOR downregulation and cell-size control. Surprisingly, regulation of cell size by cilia is independent of flow-induced calcium transients, or Akt. However, the tumour-suppressor protein Lkb1 localises in the cilium, and flow results in increased AMPK phosphorylation at the basal body. Conversely, knockdown of Lkb1 prevents normal cell-size regulation under flow conditions. Our results demonstrate that the cilium regulates mTOR signalling and cell size, and identify the cilium-basal body compartment as a spatially restricted activation site for Lkb1 signalling. 相似文献
106.
107.
Stefanie Deinhardt-Emmer Sarah Bttcher Clio Hring Liane Giebeler Andreas Henke Roland Zell Johannes Jungwirth Paul M. Jordan Oliver Werz Franziska Hornung Christian Brandt Mike Marquet Alexander S. Mosig Mathias W. Pletz Michael Schacke Jürgen Rdel Regine Heller Sandor Nietzsche Bettina Lffler Christina Ehrhardt 《Journal of virology》2021,95(10)
108.
A fragmentary cervico-pectoral lateral spine and partial humerus of an ankylosaur from the Early Cretaceous (early Valanginian) of Gronau in Westfalen, northwestern Germany, are described. The spine shows closest morphological similarities to the characteristic cervical and pectoral spines of Hylaeosaurus armatus from the late Valanginian of England. An extensive comparison of distal humeri among thyreophoran dinosaurs supports systematic differences in the morphology of the distal condyli between Ankylosauria and Stegosauria and a referral of the Gronau specimen to the former. The humerus fragment indicates a rather small individual, probably in the size range of H. armatus, and both specimens are determined herein as ?Hylaeosaurus sp.. A short overview of other purported ankylosaur material from the Berriasian-Valanginian of northwest Germany shows that, aside from the material described herein, only tracks can be attributed to this clade with confidence at present. 相似文献
109.
Ferenc Vilisics Péter Sólymos Antal Nagy Roland Farkas Zita Kemencei Elisabeth Hornung 《Biologia》2011,66(3):499-505
We studied abundance and diversity patterns of terrestrial isopod assemblages along a ‘micro-scale’ vertical gradient in sinkholes
in the Aggtelek National Park, Hungary. Time restricted manual sampling yielded ten native species, including endemic and
rare ones. Along the gradient we found no major differences in species richness and -composition, and abundance decreased
from the bottoms to the upper zones of the sinkholes. Species specific habitat preference on a vertical gradient showed two
distinct groups by indicator species analysis: occurrence of habitat “generalists” was irrespective of vertical zones while
“specialists” were restricted to the bottoms of the dolines. The latter group is formed mainly by rare species. We found that
both diversity and evenness of isopod assemblages were highest in the bottom zone. Our results draw the attention to the significance
of such common, yet undiscovered surficial depressions that can provide shelters for rare and specialist species and can provide
shelter for survival of populations under changing climatic conditions. 相似文献
110.
Rudolph M Schlereth A Körner M Feussner K Berndt E Melzer M Hornung E Feussner I 《Journal of experimental botany》2011,62(2):749-760
Oilseed germination is characterized by the mobilization of storage lipids as a carbon and energy source for embryonic growth. In addition to storage lipid degradation in germinating oilseeds via the direct action of a triacylglycerol lipase (TGL) on the storage lipids, a second degradation pathway that is dependent on a specific lipid body trilinoleate 13-lipoxygenase (13-LOX) has been proposed in several plant species. The activity of this specific 13-LOX leads first to the formation of ester lipid hydroperoxides. These hydroperoxy fatty acids are then preferentially cleaved off by a TGL and serve as a substrate for glyoxysomal β-oxidation. As a prerequisite for triacylglycerol (TAG) mobilization, a partial degradation of the phospholipid monolayer and/or membrane proteins of the oil body has been discussed. Evidence has now been found for both processes: partial degradation of the proteins caleosin and oleosin was observed and simultaneously a patatin-like protein together with transient phospholipase (PLase) activity could be detected at the oil body membranes during germination. Moreover, in vitro experiments with isolated oil bodies from mature seeds revealed that the formation of 13-LOX-derived lipid peroxides in lipid body membranes is increased after incubation with the purified recombinant patatin-like protein. These experiments suggest that in vivo the degradation of storage lipids in cucumber cotyledons is promoted by the activity of a specific oil body PLase, which leads to an increased decomposition of the oil body membrane by the 13-LOX and thereby TAGs may be better accessible to LOX and TGL. 相似文献