全文获取类型
收费全文 | 226篇 |
免费 | 17篇 |
专业分类
243篇 |
出版年
2022年 | 4篇 |
2021年 | 2篇 |
2020年 | 3篇 |
2019年 | 3篇 |
2018年 | 1篇 |
2016年 | 3篇 |
2015年 | 11篇 |
2014年 | 11篇 |
2013年 | 7篇 |
2012年 | 15篇 |
2011年 | 9篇 |
2010年 | 14篇 |
2009年 | 13篇 |
2008年 | 9篇 |
2007年 | 12篇 |
2006年 | 14篇 |
2005年 | 16篇 |
2004年 | 16篇 |
2003年 | 6篇 |
2002年 | 11篇 |
2001年 | 11篇 |
2000年 | 3篇 |
1999年 | 3篇 |
1998年 | 2篇 |
1997年 | 1篇 |
1996年 | 2篇 |
1994年 | 1篇 |
1993年 | 2篇 |
1992年 | 2篇 |
1991年 | 2篇 |
1990年 | 5篇 |
1989年 | 3篇 |
1988年 | 1篇 |
1986年 | 2篇 |
1985年 | 3篇 |
1982年 | 2篇 |
1981年 | 2篇 |
1979年 | 2篇 |
1978年 | 1篇 |
1977年 | 2篇 |
1976年 | 2篇 |
1975年 | 2篇 |
1974年 | 2篇 |
1973年 | 2篇 |
1972年 | 1篇 |
1971年 | 2篇 |
排序方式: 共有243条查询结果,搜索用时 11 毫秒
31.
T. C. Pan C. J. Horng S. R. Lin T. H. Lin C. W. Huang 《Biological trace element research》1993,38(3):233-241
Blackfoot disease (BFD) is an endemic peripheral vascular disorder resulting in gangrene of the lower extremities, especially
the feet, among residents in a limited area on the southwest coast of Taiwan. In the present study, the concentrations of
zinc, cadmium, lead, and copper in urine of BFD patients with matched normal controls are investigated by differential pulse
anodic stripping voltammetry (DPASV) on a hanging mercury drop electrode (HMDE). The analytical results indicate that urinary
copper, cadmium, and lead of the BFD patients are significantly higher than those of the controls. In addition, the patients
showed a significantly lower concentration of zinc in the urine than the normal controls. The possible connection of these
elements with the etiology of the disease is discussed. 相似文献
32.
The inhibitory effects of capillarisin on cell proliferation and invasion of prostate carcinoma cells 下载免费PDF全文
Ke‐Hung Tsui Ying‐Ling Chang Pei‐Shan Yang Chen‐Pang Hou Yu‐Hsiang Lin Bing‐Wei Lin Tsui‐Hsia Feng Horng‐Heng Juang 《Cell proliferation》2018,51(2)
Objectives
Capillarisin (Cap), an active component of Artemisia capillaris root extracts, is characterized by its anti‐inflammatory, anti‐oxidant and anti‐cancer properties. Nevertheless, the functions of Cap in prostate cancer have not been fully explored. We evaluated the potential actions of Cap on the cell proliferation, migration and invasion of prostate carcinoma cells.Materials and methods
Cell proliferation and cell cycle distribution were measured by water‐soluble tetrazolium‐1 and flow cytometry assays. The expression of cyclins, p21, p27, survivin, matrix metallopeptidase (MMP2 and MMP9) were assessed by immunoblotting assays. Effects of Cap on invasion and migration were determined by wound closure and matrigel transmigration assays. The constitutive and interlukin‐6 (IL‐6)‐inducible STAT3 activation of prostate carcinoma cells were determined by immunoblotting and reporter assays.Results
Capillarisin inhibited androgen‐independent DU145 and androgen‐dependent LNCaP cell growth through the induction of cell cycle arrest at the G0/G1 phase by upregulating p21 and p27 while downregulating expression of cyclin D1, cyclin A and cyclin B. Cap decreased protein expression of survivin, MMP‐2, and MMP‐9 and therefore blocked the migration and invasion of DU145 cells. Cap suppressed constitutive and IL‐6‐inducible STAT3 activation in DU145 and LNCaP cells.Conclusions
Our data indicate that Cap blocked cell growth by modulation of p21, p27 and cyclins. The inhibitory effects of Cap on survivin, MMP‐2, MMP‐9 and STAT3 activation may account for the suppression of invasion in prostate carcinoma cells. Our data suggest that Cap might be a therapeutic agent in treating advanced prostate cancer with constitutive STAT3 or IL‐6‐inducible STAT3 activation.33.
Hsin-Chih Lai Yu-Tze Horng Pen-Fang Yeh Jann-Yuan Wang Chin-Chung Shu Chia-Chen Lu Jang-Jih Lu Jen-Jyh Lee Po-Chi Soo 《Journal of microbiology (Seoul, Korea)》2016,54(11):761-767
Pulmonary tuberculosis (TB) is caused by Mycobacterium tuberculosis. The protein composition of sputum may reflect the immune status of the lung. This study aimed to evaluate the protein profiles in spontaneous sputum samples from patients with active pulmonary TB. Sputum samples were collected from patients with pulmonary TB and healthy controls. Western blotting was used to analyze the amount of interleukin 10 (IL-10), interferon-gamma (IFN-γ), IL-25, IL-17, perforin-1, urease, albumin, transferrin, lactoferrin, adenosine deaminase (also known as adenosine aminohydrolase, or ADA), ADA-2, granzyme B, granulysin, and caspase-1 in sputum. Results of detection of IL-10, IFN-γ, perforin-1, urease, ADA2, and caspase-1, showed relatively high specificity in distinguishing patients with TB from healthy controls, although sensitivities varied from 13.3% to 66.1%. By defining a positive result as the detection of any two proteins in sputum samples, combined use of transferrin and urease as markers increased sensitivity to 73.2% and specificity to 71.1%. Furthermore, we observed that the concentration of transferrin was proportional to the number of acid-fast bacilli detected in sputum specimens. Detection of sputum transferrin and urease was highly associated with pulmonary TB infection. In addition, a high concentration of transferrin detected in sputum might correlate with active TB infection. This data on sputum proteins in patients with TB may aid in the development of biomarkers to assess the severity of pulmonary TB. 相似文献
34.
35.
36.
37.
Li-Ching Wu Hsin-Hao Chen Jorng-Tzong Horng Chen Lin Norden E. Huang Yu-Che Cheng Kuang-Fu Cheng 《PloS one》2010,5(8)
Motivation
Mass spectrometry is a high throughput, fast, and accurate method of protein analysis. Using the peaks detected in spectra, we can compare a normal group with a disease group. However, the spectrum is complicated by scale shifting and is also full of noise. Such shifting makes the spectra non-stationary and need to align before comparison. Consequently, the preprocessing of the mass data plays an important role during the analysis process. Noises in mass spectrometry data come in lots of different aspects and frequencies. A powerful data preprocessing method is needed for removing large amount of noises in mass spectrometry data.Results
Hilbert-Huang Transformation is a non-stationary transformation used in signal processing. We provide a novel algorithm for preprocessing that can deal with MALDI and SELDI spectra. We use the Hilbert-Huang Transformation to decompose the spectrum and filter-out the very high frequencies and very low frequencies signal. We think the noise in mass spectrometry comes from many sources and some of the noises can be removed by analysis of signal frequence domain. Since the protein in the spectrum is expected to be a unique peak, its frequence domain should be in the middle part of frequence domain and will not be removed. The results show that HHT, when used for preprocessing, is generally better than other preprocessing methods. The approach not only is able to detect peaks successfully, but HHT has the advantage of denoising spectra efficiently, especially when the data is complex. The drawback of HHT is that this approach takes much longer for the processing than the wavlet and traditional methods. However, the processing time is still manageable and is worth the wait to obtain high quality data. 相似文献38.
39.
Ming‐Jhan Wu Po‐Yuan Ke John T.‐A. Hsu Chau‐Ting Yeh Jim‐Tong Horng 《Cellular microbiology》2014,16(11):1603-1618
The non‐structural protein 4B (NS4B) of the hepatitis C virus (HCV) is an endoplasmic reticulum (ER) membrane protein comprising two consecutive amphipathic α‐helical domains (AH1 and AH2). Its self‐oligomerization via the AH2 domain is required for the formation of the membranous web that is necessary for viral replication. Previously, we reported that the host‐encoded ER‐associated reticulon 3 (RTN3) protein is involved in the formation of the replication‐associated membranes of (+)RNA enteroviruses during viral replication. In this study, we demonstrated that the second transmembrane region of RTN3 competed for, and bound to, the AH2 domain of NS4B, thus abolishing NS4B self‐interaction and leading to the downregulation of viral replication. This interaction was mediated by two crucial residues, lysine 52 and tyrosine 63, of AH2, and was regulated by the AH1 domain. The silencing of RTN3 in Huh7 and AVA5 cells harbouring an HCV replicon enhanced the replication of HCV, which was counteracted by the overexpression of recombinant RTN3. The synthesis of viral RNA was also increased in siRNA‐transfected human primary hepatocytes infected with HCV derived from cell culture. Our results demonstrated that RTN3 acted as a restriction factor to limit the replication of HCV. 相似文献
40.
Chun-An Chen Meng-Yao Lu Shinn-Forng Peng Kai-Hsin Lin Hsiu-Hao Chang Yung-Li Yang Shiann-Tarng Jou Dong-Tsamn Lin Yen-Bin Liu Herng-Er Horng Hong-Chang Yang Jou-Kou Wang Mei-Hwan Wu Chau-Chung Wu 《PloS one》2014,9(1)