全文获取类型
收费全文 | 553篇 |
免费 | 45篇 |
出版年
2021年 | 11篇 |
2020年 | 4篇 |
2019年 | 11篇 |
2018年 | 9篇 |
2017年 | 7篇 |
2016年 | 15篇 |
2015年 | 14篇 |
2014年 | 16篇 |
2013年 | 20篇 |
2012年 | 21篇 |
2011年 | 27篇 |
2010年 | 18篇 |
2009年 | 17篇 |
2008年 | 21篇 |
2007年 | 15篇 |
2006年 | 22篇 |
2005年 | 16篇 |
2004年 | 14篇 |
2003年 | 21篇 |
2002年 | 25篇 |
2001年 | 10篇 |
2000年 | 18篇 |
1999年 | 10篇 |
1998年 | 8篇 |
1997年 | 4篇 |
1996年 | 4篇 |
1995年 | 7篇 |
1992年 | 10篇 |
1991年 | 10篇 |
1990年 | 9篇 |
1989年 | 7篇 |
1988年 | 7篇 |
1987年 | 7篇 |
1986年 | 9篇 |
1985年 | 11篇 |
1984年 | 14篇 |
1983年 | 13篇 |
1982年 | 6篇 |
1981年 | 7篇 |
1980年 | 4篇 |
1979年 | 6篇 |
1977年 | 8篇 |
1975年 | 5篇 |
1974年 | 6篇 |
1972年 | 8篇 |
1971年 | 6篇 |
1970年 | 4篇 |
1968年 | 5篇 |
1967年 | 4篇 |
1966年 | 4篇 |
排序方式: 共有598条查询结果,搜索用时 15 毫秒
111.
The factors influencing the uptake of a 14 C-labelled Vibrio anguillarum vaccine in direct immersion experiments with rainbow trout, Salmo gairdneri were studied. Immersion times of longer than 10 s did not increase vaccine uptake. A pre-immersion dip in a hyperosmotic solution had no effect on uptake, even at lower vaccine bath concentrations. It appeared that the head end of the fish was implicated in uptake. Vaccine uptake decreased at lower temperatures, whereas the use of an adjuvanted vaccine and a soluble vaccine preparation led to increased uptake. Larger fish took up more vaccine. 相似文献
112.
Effect of Competence Induction on Macromolecular Synthesis in a Group H Streptococcus 总被引:6,自引:2,他引:4
下载免费PDF全文
![点击此处可从《Journal of bacteriology》网站下载免费的PDF全文](/ch/ext_images/free.gif)
The effects of competence induction by competence factor (CF) on macromolecular synthesis in group H streptococcus strain Wicky were investigated. CF preparations (culture filtrates from competent group H streptococcus strain Challis) were either heated or partially purified to remove a bacteriocin. These preparations did not inhibit growth, although they induced high levels of competence in strain Wicky. The action of the CF preparations did not affect the overall rates of deoxyribonucleic acid and protein synthesis, but caused a reduction in the rates of ribonucleic acid (RNA) and peptidoglycan synthesis. When competence induction by CF was prevented, no alterations in RNA or peptidoglycan synthesis were observed, indicating that these changes are in fact related to the development of competence. 相似文献
113.
Solubilization and hydrodynamic characterization of the dihydropyridine receptor from rat ventricular muscle 总被引:3,自引:0,他引:3
The dihydropyridine receptor-calcium channel complex, prelabeled with (+)-[3H]PN200-110, was solubilized from rat heart membranes with a detergent mixture of digitonin and Triton X-100. The dissociation of (+)-[3H]PN200-110 was slow enough to permit the hydrodynamic characterization of the complex by means of sucrose gradient sedimentation and gel filtration. The hydrodynamic properties of the complex were determined in several detergents, including Tween 80, 3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonic acid (CHAPS), and digitonin. S20,w values of 12.5, 15.4, and 21.0 S were obtained in sucrose gradients prepared in Tween 80, CHAPS, and digitonin, respectively. A Stokes radius of 86-87 A was obtained in each of the three detergents. Determination of the partial specific volume of the protein-detergent complex in each case revealed that the differences in S20,w values could be explained by the differences in the properties of the bound detergent species. Partial specific volumes of 0.796, 0.730, and 0.730 ml/g, corresponding to molecular weights of 595,000, 540,000, and 740,000 were obtained for the complex in Tween 80, CHAPS, and digitonin, respectively. This indicated that Tween 80 readily exchanged for the solubilizing mixture of digitonin and Triton X-100, whereas CHAPS did not. Detergent exchange with Tween 80 made it possible to determine the fractional contribution of the receptor protein to the molecular weight of the protein-detergent complex. The molecular weight of the dihydropyridine receptor-calcium channel complex was estimated to be 370,000. The protein-detergent complex was found to have a frictional coefficient of 1.39, consistent with a large transmembrane protein. 相似文献
114.
The association of proton movement with galactose transport into subcellular membrane vesicles of Escherichia coli. 总被引:1,自引:1,他引:0
下载免费PDF全文
![点击此处可从《The Biochemical journal》网站下载免费的PDF全文](/ch/ext_images/free.gif)
1. Subcellular membrane vesicles were prepared from a strain of Escherichia coli constitutive for the GalP galactose-transport system. 2. The addition of substrates of the GalP transport system to vesicle suspensions promoted alkaline pH changes, which provided direct evidence for the coupling of sugar and proton transport. 3. Respiration-energized galactose transport was progressively inhibited at pH values above 6.0, and was abolished by agents that render the membrane permeable to protons. 4. The combined effects of valinomycin, the nigericin-like compound A217 and pH on galactose transport suggested that both delta pH and delta psi components of the protonmotive force contributed to energization of galactose transport. 5. These results substantiate the conclusion that the GalP transport system operates by a chemiosmotic mechanism. 相似文献
115.
S H Ong D Horne C K Yeung P Nickolls T Cole 《Analytical and quantitative cytology and histology / the International Academy of Cytology [and] American Society of Cytology》1987,9(5):375-382
A cell analyzer that combines the characteristics of image cytometry and flow cytometry is being designed and constructed at the University of Sydney. This paper describes the image acquisition and processing components and some preliminary applications. Cells stained by a fluorescent dye and suspended in a liquid medium are conveyed by a hydraulic system to a flow channel assembly, where they are detected and illuminated by a laser beam. A two-dimensional charge-coupled device is used to acquire the cell images. Image processing and classification is to be carried out by a special-purpose computer comprising an array of four conventional microprocessors and a highly parallel processor consisting of an array of 32 X 32 processing elements. The analyzer will be capable of using morphologic, immunologic and biochemical information to classify and sort up to 500 cells per second. Because of its unique characteristics, the instrument will be of particular use in tumor heterogeneity studies. 相似文献
116.
W. J. Brad Hanna Jane Anne Horne George H. Renninger 《Journal of comparative physiology. A, Neuroethology, sensory, neural, and behavioral physiology》1988,162(1):127-132
The circadian rhythm in the ERG amplitude of the lateral compound eye ofLimulus can be phase shifted either by general illumination or by illuminating combinations of the photoreceptor organs.
相似文献
1. | For 15-min exposures, light confined to one lateral eye, or to the median ocelli, or to the ventral photoreceptor region resulted in the smallest phase shifts. |
2. | Illuminating combinations of these organs produced larger shifts. The most effective combination tested included the median ocelli, the ventral photoreceptors, and one lateral eye. The phase shift resulting from illumination of this combination was only about one-half of the shift produced by general illumination. |
3. | These results suggest that the circadian clock also receives light information from other, unidentified, photoreceptors located outside the prosoma. |
117.
118.
119.
c-Cbl and Cbl-b Act Redundantly to Protect Osteoclasts from Apoptosis and to Displace HDAC6 from β-Tubulin,Stabilizing Microtubules and Podosomes
下载免费PDF全文
![点击此处可从《Molecular biology of the cell》网站下载免费的PDF全文](/ch/ext_images/free.gif)
Enkhtsetseg Purev Lynn Neff William C. Horne Roland Baron 《Molecular biology of the cell》2009,20(18):4021-4030
c-Cbl and Cbl-b are highly conserved adaptor proteins that participate in integrin signaling, regulating cytoskeletal organization, motility, and bone resorption. Deletion of both c-Cbl and Cbl-b in mice leads to embryonic lethality, indicating that the two proteins perform essential redundant functions. To examine the redundant actions of c-Cbl and Cbl-b in osteoclasts, we depleted c-Cbl in Cbl-b−/− osteoclasts by using a short hairpin RNA. Depleting both Cbl proteins disrupted both the podosome belt and the microtubule network and decreased bone-resorbing activity. Stabilizing the microtubules with paclitaxel or inhibiting histone deacetylase 6 (HDAC6), which destabilizes microtubules by deacetylating β-tubulin, protected both the microtubule network and the podosome belt. Examination of the mechanism involved demonstrated that the conserved four-helix bundle of c-Cbl''s tyrosine kinase binding domain bound to β-tubulin, and both c-Cbl and Cbl-b displaced HDAC6. In addition to the effects on microtubules and the podosome belt, depleting both Cbls significantly increased the levels of the proapoptotic protein Bim and apoptosis relative to the levels induced by eliminating either protein alone. Thus, both c-Cbl and Cbl-b promote bone resorption via the stabilization of microtubules, allowing the formation of the podosome belt in osteoclasts, and by promoting osteoclast survival. 相似文献
120.
The immobilization isotherms of Clostridium thermocellum cells on bituminous coal particles of approximately 0.15- to 0.18-mm diameter were experimentally measured at 60, 45, and 30 degrees C with a pH value of 7.0 and with pH values of 6.0 and 5.0 at 60 degrees C. The immobilization data were correlated into Langmuir forms and their characteristic coefficients were obtained. A method to obtain thermodynamic quantities delta G, delta H, and delta S for the immobilization is also demonstrated. 相似文献