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91.
92.
Isao kubo James A. Klocke Shoji Asano 《Bioscience, biotechnology, and biochemistry》2013,77(8):1925-1927
γ-Glutamy Icy steine synthetase was purified from E. coli B. The enzyme had a molecular weight of 5.5 × 104 and required only magnesium ion for activity. The optimal pH and temperature for reaction were 8.5 and 45°C, respectively. The Km values for l-glutamate, l-cysteine, and ATP were 0.50, 0.09, and 0.01 mm, respectively. GTP and UTP were also used as energy sources. The enzyme activity was inhibited by phosphate anions and by various sulfhydryl reagents. Unlike the enzyme from mammalian tissues, the E. coli B enzyme was not inhibited by α-alkyl analogues of methionine. The enzyme was feedback inhibited by reduced glutathione, although oxidized glutathione had no inhibitory effect. 相似文献
93.
Isao Shibuya Hiroko Honda Bunji Maruo 《Bioscience, biotechnology, and biochemistry》2013,77(1):111-114
An NAD linked formate dehydrogenating enzyme which catalyzed the last step of methanol oxidation system was extracted from the methanol-grown Kloeckera sp. No. 2201. The specific activity of the enzyme in the extract of methanol-grown cells was found to be considerably higher than that of the glucose-grown cells. The enzyme was purified about 35-fold from the extract of methanol-grown cells by heat treatment, column chromatographies on DEAE-cellulose and on hydroxylapatite, and Sephadex G-200 gel filtration. The purified enzyme was shown to be homogeneous by analyses with electrophoresis and ultracentrifuga-tion. The purified enzyme was a kind of NAD: formate oxidoreductase (EC, 1.2.1.2) which catalyzed specifically the oxidation of formate to carbon dioxide. The Km values were 22 mm for formate and 0.1 mm for NAD. The enzyme was inactivated by potassium cyanide, sodium azide, and p-chloromercuribenzoate but not by any metal-chelating reagents tested. Other general properties of the enzyme were also investigated. 相似文献
94.
Keiichi Nitta Chikako Takura Isao Yamamoto Yuzuru Yamamoto Junzo Imai Saburo Yamatodani 《Bioscience, biotechnology, and biochemistry》2013,77(12):813-827
The chemical structure of oospolactone which is the metabolic product of Oospora astringenes was confirmed by the synthetical approach. 相似文献
95.
Kazuko Oshima-Oba Isao Sugiura Ikuzo Uritani 《Bioscience, biotechnology, and biochemistry》2013,77(4):586-591
1. It was demonstrated by silica gel thin layer chromatography that leucine-U-14C was incorporated into furanoterpenes, e. g. ipomeamarone, in sweet potato root tissue infected by Ceratocystis fimbriata.2. Further proof for ipomeamarone synthesis from leucine-U-14C was obtained by the constancy of the specific radioactivity of ipomeamarone semicarbazone through repetitive crystallization.3. The synthetic pathway of ipomeamarone from leucine was found to be connected with the synthetic pathway from acetate at least at some steps.4. Leucine-U-14C was incorporated into both saponifiable and non-saponifiable materials in the same way as acetate-2-14C. 相似文献
96.
Changes in the small intestinal mucin contents in rats were evaluated by two methods, viz., a newly established ELISA and a method based on the measurement of O-linked oligosaccharide chains (OSC) as a mucin marker. Significant correlation was observed between the values of ELISA-derived mucins and OSC. The results confirm the usefulness of measurement of OSC as an alternative method for mucin determination. 相似文献
97.
Takashi Nara Isao Kawamoto Masanaru Misawa Shukuo Kinoshita 《Bioscience, biotechnology, and biochemistry》2013,77(8):956-962
Brevibacterium insectiphilium KY 3446 (Steinhous, Breed AHU 1401) was found to accumulate IMP from hypoxanthine and UMP from uracil, respectively. This strain is thus considered to present the fourth example in salvage-type fermentation, in addition to Micrococcus sodonensis, Arthrobacter citreus and Brevibacterium ammoniagenes reported previously.IMP from adenine and UMP from cytosine were also produced by KY 3446, respectively. Further, the addition of inosine and adenosine instead of the bases also caused IMP accumulation.This strain grew well on sucrose medium, and produced IMP and UMP in higher yields on sucrose than on glucose medium.Excessive amounts of Mn2+ stimulated growth, but markedly inhibited IMP production. The optimal concentration of Mn2+ for IMP accumulation induced morphogenetic alterations from normal and small to abnormal and large cells. 相似文献
98.
Yosuke Akiba Akiko Mizuta Yoshito Kakihara Juri Nakata Jun Nihara Isao Saito Hiroshi Egusa Makio Saeki 《Biochemistry and Biophysics Reports》2016
Osteoclasts are multinucleated cells with bone resorption activity that is crucial for bone remodeling. RANK‐RANKL (receptor activator of nuclear factor κB ligand) signaling has been shown as a main signal pathway for osteoclast differentiation. However, the molecular mechanism and the factors regulating osteoclastogenesis remain to be fully understood. In this study, we performed a chemical genetic screen, and identified a Cdks/GSK-3β (cyclin-dependent kinases/glycogen synthase kinase 3β) inhibitor, kenpaullone, and two Cdks inhibitors, olomoucine and roscovitine, all of which significantly enhance osteoclastogenesis of RAW264.7 cells by upregulating NFATc1 (nuclear factor of activated T cells, cytoplasmic 1) levels. We also determined that the all three compounds increase the number of osteoclast differentiated from murine bone marrow cells. Furthermore, the three inhibitors, especially kenpaullone, promoted maturation of cathepsin K, suggesting that the resorption activity of the resultant osteoclasts is also activated. Our findings indicate that inhibition of GSK-3β and/or Cdks enhance osteoclastogenesis by modulating the RANK–RANKL signaling pathway. 相似文献
99.
100.
Sunaho Okada Sadaf A. Raja Jonathan Okerblom Aayush Boddu Yousuke Horikawa Supriyo Ray 《Cell cycle (Georgetown, Tex.)》2019,18(11):1268-1280
Caveolin-1 (Cav-1) is an integral membrane protein that plays an important role in proliferative and terminally differentiated cells. As a structural component of Caveolae, Cav-1 interacts with signaling molecules via a caveolin scaffolding domain (CSD) regulating cell signaling. Recent reports have shown that Cav-1 is a negative regulator in tumor metastasis. Therefore, we hypothesize that Cav-1 inhibits cell migration through its CSD. HeLa cells were engineered to overexpress Cav-1 (Cav-1 OE), Cav-1 without a functional CSD (?CSD), or enhanced green fluorescent protein (EGFP) as a control. HeLa cell migration was suppressed in Cav-1 OE cells while ?CSD showed increased migration, which corresponded to a decrease in the tight junction protein, zonula occludens (ZO-1). The migration phenotype was confirmed in multiple cancer cell lines. Phosphorylated STAT-3 was decreased in Cav-1 OE cells compared to control and ?CSD cells; reducing STAT-3 expression alone decreased cell migration. ?CSD blunted HeLa proliferation by increasing the number of cells in the G2/M phase of the cell cycle. Overexpressing the CSD peptide alone suppressed HeLa cell migration and inhibited pSTAT3. These findings suggest that Cav-1 CSD may be critical in controlling the dynamic phenotype of cancer cells by facilitating the interaction of specific signal transduction pathways, regulating STAT3 and participating in a G2/M checkpoint. Modulating the CSD and targeting specific proteins may offer potential new therapies in the treatment of cancer metastasis. 相似文献