Effect of GPIIb-IIIa complex ligands on calcium ion movement and cytoskeleton organization in activated platelets

We studied the influence of the occupancy of the fibrinogen receptor (GP IIb-IIIa complex) on two early aspects of agonist induced platelet activation: the increase of the intracellular Ca2+ concentration and the cytoskeleton reorganization. A monoclonal antibody, a peptide containing the RGD sequence and fibrinogen purified from human plasma were used as GP IIb-IIIa ligands. The obtained results demonstrated that fibrinogen receptor occupancy inhibits Ca2+ movement and cytoskeleton reorganization caused by low thrombin concentration and ADP.     

Characterization of a Human Apolipoprotein A-I Construct Expressed in a Bacterial System

Apolipoprotein A-I (apoA-I) is the major protein component of high density lipoproteins. This protein has key functions in lipoprotein metabolism and its plasma concentration is inversely correlated with the incidence of atherosclerosis and cardiovascular diseases. There is an increasing need to develop methods for efficient production of recombinant apoA-I for using it in basic research or pharmacological therapy. An apoA-I variant lacking two amino acid residues at the N-terminus can be easily produced by bacterial expression. We report here the characterization of this variant comparing its properties with those of the protein isolated from human serum. The results validate the use of this variant in future assays and investigations.     

Oxidation of substituted benzyl alcohols to carboxylic acids by Nocardia corallina B-276

Whole cells of Nocardia corallina B-276, oxidized 21 substituted benzyl alcohols, at 1 mM scale, to carboxylic acids at 28-30°C, giving yields of products from 5 to 77%.     

The shaping of intrinsic membrane potential oscillations: positive/negative feedback,ionic resonance/amplification,nonlinearities and time scales

The generation of intrinsic subthreshold (membrane potential) oscillations (STOs) in neuronal models requires the interaction between two processes: a relatively fast positive feedback that favors changes in voltage and a slower negative feedback that opposes these changes. These are provided by the so-called resonant and amplifying gating variables associated to the participating ionic currents. We investigate both the biophysical and dynamic mechanisms of generation of STOs and how their attributes (frequency and amplitude) depend on the model parameters for biophysical (conductance-based) models having qualitatively different types of resonant currents (activating and inactivating) and an amplifying current. Combinations of the same types of ionic currents (same models) in different parameter regimes give rise to different types of nonlinearities in the voltage equation: quasi-linear, parabolic-like and cubic-like. On the other hand, combinations of different types of ionic currents (different models) may give rise to the same type of nonlinearities. We examine how the attributes of the resulting STOs depend on the combined effect of these resonant and amplifying ionic processes, operating at different effective time scales, and the various types of nonlinearities. We find that, while some STO properties and attribute dependencies on the model parameters are determined by the specific combinations of ionic currents (biophysical properties), and are different for models with different such combinations, others are determined by the type of nonlinearities and are common for models with different types of ionic currents. Our results highlight the richness of STO behavior in single cells as the result of the various ways in which resonant and amplifying currents interact and affect the generation and termination of STOs as control parameters change. We make predictions that can be tested experimentally and are expected to contribute to the understanding of how rhythmic activity in neuronal networks emerge from the interplay of the intrinsic properties of the participating neurons and the network connectivity.     

Distribution patterns of flora and fauna in southern Chilean Coastal rain forests: Integrating Natural History and GIS

Knowledge of species richness centers is necessary for the design of conservation areas. In this study, we present a GIS analysis of two years of field data on animal and plant diversity distributions in evergreen, coastal rain forests of southern Chile (39°30′–41°25′ S). Despite their high endemism, these forests have remained largely unprotected. Field records were complemented with data from museum collections and scientific literature. We used selected environmental variables (evapotranspiration, altitude) and, in some cases, forest types as predictors of species distributions. Our study focused on the distribution of forest bryophytes, vascular plants, soil invertebrates, amphibians and birds. We generated distributional maps for each taxa based on their field records in the study area, complemented by natural history information, except in the case of bryophytes and soil invertebrates. In general, species richness was lower at 600 m elevation or above for all the taxa studied. Species richness tends to increase in the northern sector of the study area. We observed a greater richness of vascular plants near rivers and streams, and noted important floristic differences between west and east-facing slopes of the Coastal Range, with more species in the oriental side. Because species in high altitude forests are not a subset of those found at lower elevations, we propose that conservation strategies should prioritize the protection of the entire altitudinal gradient of the southern Coastal Range, especially in the more diverse oriental and northern sectors.     

A New Basal Subfamily of mariner Elements in Ceratitis rosa and Other Tephritid Flies

Several copies of highly related transposable elements, Crmar2, Almar1, and Asmar1, are described from the genomes of Ceratitis rosa, Anastrepha ludens, and A. suspensa, respectively. One copy from C. rosa, Crmar2.5, contains a full-length, uninterrupted ORF. All the other copies, from the three species contain a long deletion within the putative ORF. The consensus Crmar2 element has features typical of the mariner/Tc1 superfamily of transposable elements. In particular, the Crmar2 consensus encodes a D,D41D motif, a variant of the D,D34D catalytic domain of mariner elements. Phylogenetic analysis of the relationships of these three elements and other members of the mariner/Tc1 superfamily, based on their encoded amino acid sequences, suggests that they form a new basal subfamily of mariner elements, the rosa subfamily. BLAST analyses identified sequences from other diptera, including Drosophila melanogaster, which appear to be members of the rosa subfamily of mariner elements. Analyses of their molecular evolution suggests that Crmar2 entered the genome of C. rosa in the recent past, a consequence of horizontal transfer.