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841.
Raleigh R. Hood Xinsheng Zhang Patricia M. Glibert Michael R. Roman Diane K. Stoecker 《Harmful algae》2006,5(4):459
A semi-idealized marine ecosystem model, designed as a heuristic tool for exploring the population dynamics of non-inducible versus toxic forms of Pfiesteria is described. The model is based on empirical evidence suggesting that these differing functional types of Pfiesteria also differ substantially in terms of what they eat and how they utilize it to optimize their growth. Non-inducible strains are similar to other mixotrophic dinoflagellates, whereas toxic strains may consume organic matter and detritus, produce toxins and attack fish. In our model formulation we represent these differences in a simplified way: the non-inducible strain is kleptochloroplastidic and it can take up DIN, but it cannot utilize DON, whereas the toxic strain is heterotrophic, it cannot utilize DIN, but it can utilize DON directly. These differences give rise to very different impacts on prey and nutrient concentrations in our model. Under high DIN/DON ratio conditions, the non-inducible cells grew much faster and were therefore more likely to bloom, but this advantage is substantially mitigated when the DIN/DON ratio is low. A turbulence parameterization was also incorporated into our model. The effect of this was to reduce the grazing rate of Pfiesteria when turbulence levels are high. According to our model, increased turbulence is more detrimental to the toxic functional type because it grows more slowly. The further imposition of microzooplankton grazing in the model showed that top-down control effects can be very significant, which is consistent with both laboratory and field studies and the general idea that plankton blooms can only happen in the absence of substantial grazing control. In general, our model results suggest that non-toxic blooms are more likely to occur in more turbulent inorganic-nutrient rich conditions, which are often found in more open coastal and estuarine waters that are subject to high inorganic loading. In contrast, toxic blooms are more likely to occur in calm, organic-nutrient rich conditions, which are often found in shallow, protected tributaries that are subject to high organic nutrient loading. Our model results also support the idea that the absence of strong grazing pressure is a prerequisite to bloom formation for both non-inducible and toxic strains of Pfiesteria. These results are generally consistent with observed patterns of toxic Pfiesteria blooms in Chesapeake Bay, the Neuse River of North Carolina and many other coastal and estuarine environments. 相似文献
842.
Amanda K. Weaver Glen Ray Hood Michael Foster Scott P. Egan 《Ecology and evolution》2020,10(18):10207-10218
Complex interactions within multitrophic communities are fundamental to the evolution of individual species that reside within them. One common outcome of species interactions are fitness trade‐offs, where traits adaptive in some circumstances are maladaptive in others. Here, we identify a fitness trade‐off between fecundity and survival in the cynipid wasp Callirhytis quercusbatatoides that induces multichambered galls on the stem of its host plant Quercus virginiana. We first quantified this trade‐off in natural populations by documenting two relationships: a positive association between the trait gall size and fecundity, as larger galls contain more offspring, and a negative association between gall size and survival, as larger galls are attacked by birds at a higher rate. Next, we performed a field‐based experimental evolution study where birds were excluded from the entire canopy of 11 large host trees for five years. As a result of the five‐year release from avian predators, we observed a significant shift to larger galls per tree. Overall, our study demonstrates how two opposing forces of selection can generate stabilizing selection on a critical phenotypic trait in wild populations, and how traits can evolve rapidly in the predicted direction when conditions change. 相似文献
843.
Takahashi Mark; Chesley Alan; Freyssenet Damien; Hood David A. 《American journal of physiology. Cell physiology》1998,274(5):C1380
We previouslydemonstrated that subsarcolemmal (SS) and intermyofibrillar (IMF)mitochondrial subfractions import proteins at different rates. Thisstudy was undertaken to investigate1) whether protein import is alteredby chronic contractile activity, which induces mitochondrialbiogenesis, and 2) whether these two subfractions adapt similarly. Using electrical stimulation (10 Hz, 3 h/day for 7 and 14 days) to induce contractile activity, we observedthat malate dehydrogenase import into the matrix of the SS and IMFmitochondia isolated from stimulated muscle was significantly increasedby 1.4- to 1.7-fold, although the pattern of increase differed for eachsubfraction. This acceleration of import may be mitochondrialcompartment specific, since the import of Bcl-2 into the outer membranewas not affected. Contractile activity also modified the mitochondrialcontent of proteins comprising the import machinery, as evident fromincreases in the levels of the intramitochondrial chaperone mtHSP70 aswell as the outer membrane import receptor Tom20 in SS and IMFmitochondria. Addition of cytosol isolated from stimulated or controlmuscles to the import reaction resulted in similar twofold increases inthe ability of mitochondria to import malate dehydrogenase, despiteelevations in the concentration of mitochondrial import-stimulatingfactor within the cytosol of chronically stimulated muscle. Theseresults suggest that chronic contractile activity modifies the extra- and intramitochondrial environments in a fashion that favors the acceleration of precursor protein import into the matrix of the organelle. This increase in protein import is likely an important adaptation in the overall process of mitochondrial biogenesis. 相似文献
844.
845.
Phosphorescent zinc sulfide is a nonradioactive alternative for making orientation and identification markings on autoradiograms. Measurements with a luminometer show that light emission is linear with respect to ZnS concentration. A minimum activation time of 5 s has been determined, using an incandescent lamp as a light source. Emission decay kinetics show light emissions reached background levels within minutes, depending on the ZnS concentration. This time period is sufficient for X-ray films to be permanently marked. Because of its efficiency and nontoxicity, this autoradiogram marker could be extremely useful in many protocols, including high-throughput radioactive DNA sequencing. This nonradioactive marker will also be useful in protocols utilizing nonradioactive detection systems, such as those calling for biotinylated and chemiluminescent probes. 相似文献
846.
847.
We have devised a reliable procedure for the separation of three beta-lactamases of isoelectric focusing points (pI), 5.4, 6.5, and 7.9 by Fast Protein Liquid Chromatography (FPLC System). All of these enzymes were transferable and originated from a ceftazidime and cefotaxime resistant Klebsiella pneumoniae isolated in Bombay, India. The complete separation of the enzymes, achievable by this method, allowed each of the different individual beta-lactamases to be characterized biochemically. This analysis revealed that the enzymes of pI 6.5 and pI 7.9 hydrolysed ceftazidime and cefotaxime, and were responsible for the resistance of K. pneumoniae, and its Escherichia coli J53-2 transconjugant to third generation cephalosporins. The enzyme of pI 5.4 was the TEM-1 beta-lactamase. The beta-lactamase of pI 7.9 appears quite different from any previously reported third generation cephalosporin hydrolysing beta-lactamase, and consequently given the preliminary designation DJP-1. This is also the first example of extended spectrum hydrolysing beta-lactamases found in Asia. 相似文献
848.
Ultrastructure of scorpion spermatozoa with atypical axonemes 总被引:2,自引:0,他引:2
849.
850.
Reed L. Wadley Carol J. Pierce Colfer Ian G. Hood 《Human ecology: an interdisciplinary journal》1997,25(2):243-271
Hunting by Iban forest farmers in West Kalimantan, Indonesia, is an important part of their subsistence economy, and as such became a focus of study as part of a conservation project in the Danau Sentarum Wildlife Reserve. In this paper, we examine Iban hunting of nonhuman primates with comparison to other large mammals. We analyze rates of encounter and capture, comparing encounters, hunting trips, and animal numbers. Information on habitats hunted shows the importance of secondary and old growth forest. Also examined are Iban attitudes, game preferences, and taboos. The significance of these findings is discussed with regard to the threats to wildlife from increases in the use of shotguns, human population, and habitat destruction, showing that conservation may be aided by promoting or enhancing certain aspects of the traditional Iban agroforestry system. 相似文献