全文获取类型
收费全文 | 32143篇 |
免费 | 2849篇 |
国内免费 | 22篇 |
专业分类
35014篇 |
出版年
2023年 | 162篇 |
2022年 | 371篇 |
2021年 | 784篇 |
2020年 | 423篇 |
2019年 | 563篇 |
2018年 | 670篇 |
2017年 | 544篇 |
2016年 | 974篇 |
2015年 | 1648篇 |
2014年 | 1719篇 |
2013年 | 2027篇 |
2012年 | 2674篇 |
2011年 | 2718篇 |
2010年 | 1653篇 |
2009年 | 1365篇 |
2008年 | 2042篇 |
2007年 | 2011篇 |
2006年 | 1953篇 |
2005年 | 1681篇 |
2004年 | 1708篇 |
2003年 | 1504篇 |
2002年 | 1461篇 |
2001年 | 299篇 |
2000年 | 226篇 |
1999年 | 293篇 |
1998年 | 311篇 |
1997年 | 221篇 |
1996年 | 209篇 |
1995年 | 171篇 |
1994年 | 158篇 |
1993年 | 158篇 |
1992年 | 137篇 |
1991年 | 157篇 |
1990年 | 125篇 |
1989年 | 116篇 |
1988年 | 97篇 |
1987年 | 88篇 |
1986年 | 99篇 |
1985年 | 107篇 |
1984年 | 109篇 |
1983年 | 109篇 |
1982年 | 122篇 |
1981年 | 120篇 |
1980年 | 97篇 |
1979年 | 71篇 |
1978年 | 53篇 |
1976年 | 54篇 |
1975年 | 50篇 |
1974年 | 52篇 |
1973年 | 51篇 |
排序方式: 共有10000条查询结果,搜索用时 0 毫秒
121.
122.
Ewa Nizankowska Angelita Q. Sheridan Marie H. Maile Carol J. Cross Rafal Nizankowski Krystyna Prochowska Andrew Szczeklik 《Prostaglandins & other lipid mediators》1985,29(3):349-362
We evaluated in a double-blind study the bronchodilatory properties of 2-decarboxy-2-hydroxymethyl prostaglandin E1 (PGE1-carbinol), described recently as a nonirritant bronchodilator in animals. Fifteen asthmatic patients received by inhalation single doses of 1, 10, and 30 μg PGE1-carbinol, 55 μg PGE2, and placebo (10% ethanol in normal saline, which was also used as diluent for the PGs). Such pulmonary function tests as forced expiratory volume in 1 second, forced vital capacity, and maximal expiratory flow were monitored during 2 hours following inhalation of each compound. 10 and 30 μg PGE1-carbinol produced significant but short-acting bronchodilation, similar to that caused by 55 μg PGE2. One-third of the patients reported mild cough and throat irritation during and shortly after inhalation of 30 μg PGE1-carbinol or 55 μg PGE2. Placebo and 1 μg PGE1-carbinol produced minimal side effects, but neither agent caused bronchodilation. In an adjunctive, unblinded trial, the same patients received 400 μg fenoterol. Fenoterol caused greater bronchodilation 15 and 30 minutes after inhalation than did the PGs in the double-blind study. 相似文献
123.
Ability to raise and lower heart rate (HR) on instruction was tested before and after unidirectional biofeedback training in two groups of 10 male volunteers. Instructional control was assessed in 2-min trials before training, and after 5 and 10 biofeedback trials of increasing (Group I) and decreasing (Group D) HR. The magnitude of HR elevations produced by Group D diminished following training, while modifications in Group I were unchanged. This negative transfer effect is discussed in relation to whether voluntary speeding and slowing HR reflect distinct capacities. 相似文献
124.
The major factors thought to control the distribution of associations on the sea cliffs in Britain are discussed in relation to the zonation of cliff vegetation, and some experiments to investigate the effects of these factors are described.The seeds of the maritime cliff species are able to germinate in higher salinities than those of closely related inland species.Relative growth rates of maritime cliff species indicate stimulation at low salinities over non-saline conditions, and less reduction in growth at higher salinities than those of closely related inland species.Increasing salinity reduces both photosynthesis and dark respiration in Lavatera arborea. Mesophyll and stomatal resistances are increased while transpiration is reduced.The distribution of ions within Lavatera arborea grown at different salinities indicates differential accumulation between young leaves, old leaves and roots.Nomenclature follows Clapham, Tutin & Warburg (1962). 相似文献
125.
126.
We have reconstituted pigment-protein complexes isolated from Rhodopseudomonas palustris photosynthetic membranes into phospholipid liposomes. The various complexes were tested for their ability to promote adhesion of the liposome membrane in the presence and absence of Mg2+ ions. Samples containing a reaction center (RC)/light-harvesting I (LHI) complex appeared to stack in a manner resembling control thylakoids in 2 and 5 mM Mg2+. We also tested for the effects of Mg2+ on detergent extractablity of pigment-protein complexes from intact membranes. Mg2+ sharply reduced the amount of LHI solubilized from membranes, while having little effect on the extractability of the light harvesting II complex (LHII) and the RC. Based on these results we suggest that LHI is the principal adhesion factor of R. palustris thylakoids.Abbreviations LHC
light harvesting complex
- OG
octyl glucoside
- RC
reaction center
This paper is dedicated to Professor G. Drews on the occasion of his 60th birthday 相似文献
127.
Noni E. MacDonald Susan Collison Norman Wolfish Peter N. McLaine Andrew M. Mackenzie 《CMAJ》1985,133(12):1211-1213
To determine the effectiveness of precleansing with chlorhexidine gluconate-cetrimide in reducing the contamination rate of bagged urine specimens, 62 infants admitted to a children''s hospital were randomly assigned to either receive (32 infants) or not receive (30) cleansing before bag application. Perimeatal swabs were taken before bag application and, in the treated group, after cleansing. Of the specimens from the treated group 69% were found to be contaminated, compared with 73% of those from the no-cleansing group. Chlorhexidine was ineffective in eliminating the perimeatal flora in 75% of the infants. The same organisms were present on the perimeatal swab and in the urine specimen in 95% of the infants in the treated group and 96% of those in the no-cleansing group. To estimate the contamination rate of urine specimens routinely cultured in the laboratory, 200 consecutive specimens (142 midstream and 58 bagged) were cultured. The contamination rate of the midstream urine specimens was 15%, compared with 66% for the bagged speciments. The cost of laboratory processing of contaminated bagged urine specimens at the hospital in 1983 may have been as high as $13 365. Chlorhexidine cleansing does not appear to be cost-effective. Further randomized controlled studies are needed to evaluate the effectiveness of other cleansing agents in reducing the contamination rate of bagged urine specimens. 相似文献
128.
One heavy chain variable region gene segment subfamily in the BALB/c mouse contains 500-1000 or more members 总被引:24,自引:0,他引:24
Nucleic acid hybridization studies suggest that 500-1000 or more heavy chain variable (VH) gene segments related to one VH gene segment (J558) are present in the genome of the BALB/c mouse. The implications of this result regarding the overall size, sequence organization, and evolution of the mouse family of VH gene segments are discussed. 相似文献
129.
The cytochromeb
6/f complex of higher plant chloroplasts is uniformly distributed throughout both appressed and nonappressed thylakoids, in contrast to photosystem II and photosystem I, the other major membrane protein complexes involved in electron transport. We discuss how this distribution is likely to affect interactions of the cytochromeb
6/f complex with other electron transport components because of the resulting local stoichiometries, and how these may affect the regulation of electron transport. 相似文献
130.
Rita Józsa M.D. Sándor Vigh Béla Mess Andrew V. Schally 《Cell and tissue research》1986,244(3):681-685
Summary In chicken embryos of different ages and in young chickens after hatching, neural elements reacting with antibodies generated against synthetic ovine corticotropin-releasing factor (CRF) were studied by means of the peroxidase-anti-peroxidase (PAP) technique at the lightmicroscopic level. CRF-immunoreactivity was first observed in perikarya located in the periventricular part of the hypothalamus on the 14th day of the incubation period. CRF-containing neural elements were detected on the same day of incubation in the external zone of the median eminence, but not in all investigated animals. In extrahypothalamic sites, immunoreactive perikarya were demonstrable in the central gray of the mesencephalon on the 15th day of incubation. Furthermore, immunoreactive cells appeared in other brain regions such as nucleus accumbens and dorsomedial nucleus of the thalamus after hatching. The present observations provide information regarding the functional development of the hypothalamo-hypophyseal-adrenal axis in the chick embryo. 相似文献