Increased numbers of P63-positive/CD117-positive cells in advanced adenoid cystic carcinoma give a poorer prognosis

ABSTRACT: ObjectivesThis study consisted of two parts. One part was to analyze the survival rates of adenoid cystic carcinoma (ACC) in Chinese and explain the difference between our data and the literature. The other was to analyze the relationship between the expression of CD117 and the histological grade and the prognosis. METHODS: A retrospective study of 80 ACC patients was performed. Clinical data were collected, and p63, CD117 were detected by immunohistochemical staining. RESULTS: Eighty patients received follow-ups 3 to 216 months after initial diagnosis. ACC occurred in the lacrimal gland (26.3%, n = 21), nasal cavity and parasinus (33.8%, n = 27) and other sites (40.0%, n = 33). The 5-year and 10-year survival rates were 66.41% and 10.16%, respectively. Over expression of CD117 was detected in p63-negative cells in 94.3% of cases and in p63-positive cells in 45.8%. The expression of CD117 in p63-positive cells was significantly associated with the histological grade (P<0.001) and prognosis (P = 0.037) in patients in the advanced stage. CONCLUSIONS: ACC had a good 5-year survival but poor 10-year survival in Chinese, which differed from the occidental data. More p63+/CD117+ cells were associated with a higher histological grade and poorer outcome.Virtual slidesThe virtual slide(s) for this article can be found here: http://www.diagnosticpathology.diagnomx.eu/vs/1701457278762097.     

Label-free biosensor: a novel phage-modified Light Addressable Potentiometric Sensor system for cancer cell monitoring

Early diagnosis has become the most important factor influencing the cancer's curing efficiency in clinical medicine. Here we present a new way for detection of cancer markers and cancer cells based on phage-modified Light Addressable Potentiometric Sensor (phage-LAPS). Phages were immobilized on the Si₃N₄ chip surfaces covalently. Using the back-illuminating system, this phage-LAPS is tested to detect human phosphatase of regenerating liver-3 (hPRL-3) in the concentrations of 0.04–400 nM, and the mammary adenocarcinoma cell (MDAMB231) in the concentrations of 0–105 mL. The maximum responding signal is about 10 and 60 μV, respectively. The results demonstrated that the system was more applicable to detection of cancer cell than that of cancer biomarkers. This work might show potential application in clinic assays of cancer and the study of phage–cell interaction.     

一株红树林曲霉菌Aspergillus sp.WHUF0343的次级代谢产物研究

【目的】研究分离自三亚亚龙湾红树林根系土壤的真菌Aspergillus sp. WHUF0343的次级代谢产物及其生物活性。【方法】利用硅胶柱层析、Sephadex LH-20凝胶柱层析和半制备高效液相色谱等技术对该菌的发酵产物进行分离纯化;综合利用核磁共振波谱(NMR)和质谱(MS)等现代波谱技术以及与文献数据对照确定化合物的结构;分别采用肉汤微量稀释检测法和MTS法对化合物进行抗菌和肿瘤细胞毒活性测试。【结果】从真菌Aspergillus sp.WHUF0343的发酵产物共分离并鉴定了10个化合物,分别为isoechinulin A (1)、neoechinulin A (2)、neoechinulin E (3)、preechinulin (4)、neoechinulin D (5)、variecolorin J (6)、dehydroechinulin (7)、questinol (8)、emodin (9)和catenarin (10)。活性评价显示化合物2、9和10对幽门螺杆菌(Helicobacter pylori)和金黄色葡萄球菌(Staphylococcus aure...     

Preoperative T Staging of Potentially Resectable Esophageal Cancer: A Comparison between Free-Breathing Radial VIBE and Breath-Hold Cartesian VIBE,with Histopathological Correlation

Purpose: To compare the T staging of potentially resectable esophageal cancer using free-breathing radial VIBE (r-VIBE) and breath-hold Cartesian VIBE (C-VIBE), with pathologic confirmation of the T stage. Materials and Methods: Fifty patients with endoscopically proven esophageal cancer and indeterminate T1/T2/T3 stage by CT scan were examined on a 3-T scanner. The MRI protocol included C-VIBE at 150 seconds post–IV contrast, immediately followed by a work-in-progress r-VIBE with identical spatial resolution (1.1 mm × 1.1 mm × 3.0 mm). Two independent readers assigned a T stage on MRI according to the 7th edition of UICC-AJCC TNM Classification, and postoperative pathologic confirmation was considered the gold standard. Interreader agreement was also calculated. Results: The T staging agreement between both VIBE techniques and postoperative pathologic T staging was 52% (26/50) for C-VIBE, 80% (40/50) for r-VIBE for reader 1, and 50% (25/50), 82% (41/50) for reader 2, respectively. For the esophageal cancer with invading lamina propria, muscularis mucosae, or submucosa (T1 stage), r-VIBE achieved 86% (12/14) agreement for both readers 1 and 2. For invasion of muscularis propria (T2 stage), r-VIBE achieved 83% (25/30) for both readers 1 and 2, whereas for the invasion of adventitia (T3 stage), r-VIBE could only achieve agreement in 50% (3/6) and 67% (4/6) for readers 1 and 2, respectively. Conclusion: Contrast-enhanced free-breathing r-VIBE is superior to breath-hold CVIBE in T staging of potentially resectable esophageal cancer, especially for T1 and T2.     

光温因子与杂交水稻生态群体的产量和品质性状的典型相关分析

以54个杂交水稻组合的F₁生态群体为材料,应用典型相关分析方法分析了光温因子与杂交水稻的产量和品质性状的典型相关性.结果表明,光温因子与杂交水稻的产量构成因素、产量性状和品质性状间均有显著典型相关关系,尤其是营养生长期的光温因子与产量性状的第一典型相关系数为λ₁=0.9975^**,其相关信息占两组性状间总相关信息的99.96%,引起的产量变异为99.50%;光温因子主要通过对每穗实粒数、每穗总粒数和结实率的作用而影响杂交水稻的产量;不同生育期起主导作用的光温因子并不相同,营养生长期为积温和有效积温,生殖生长期为极端温差和日照时数;光温因子对杂交水稻品质性状的精米率和整精米率影响明显;不同生育期影响品质性状的主导光温因子不同,灌浆期前是积温,之后是极端温差和日照时数.     

幼苗期不同水稻材料应答盐胁迫的生理差异分析

为了解耐盐水稻HD96-1幼苗期耐盐生理调控特性，该研究以籼型水稻HD96-1(耐盐性强)和93-11(耐盐性弱)为材料，采用营养液水培法，设置3种NaCl盐浓度(0、60、120 mmol·L^-1),对3叶期幼苗进行了7 d盐处理，测定和分析了两个材料的生长参数和生理生化指标。结果表明：(1)在盐胁迫下，水稻幼苗均表现为株高和假茎宽减小，根冠比增加；与93-11比，HD96-1株高和茎宽减小幅度低，根冠比增加幅度高；地上部和根系干重，HD96-1增加，而93-11减少。(2)盐胁迫后，水稻幼苗的丙二醛(MDA)、超氧阴离子(O₂^-)和过氧化氢(H₂O₂)含量均上升，但HD96-1增幅较93-11低。(3)在盐胁迫下，水稻幼苗体内的超氧化物歧化酶(SOD)、过氧化物酶(POD)、过氧化氢酶(CAT)、抗坏血酸过氧化物酶(APX)的活性，以及抗坏血酸(AsA)、谷胱甘肽(GSH)、脯氨酸、可溶性糖和可溶性蛋白的含量均升高，HD96-1增幅高于93-11。综上表明，两个水稻材料幼苗...     

hmChIP: a database and web server for exploring publicly available human and mouse ChIP-seq and ChIP-chip data

hmChIP is a database of genome-wide chromatin immunoprecipitation (ChIP) data in human and mouse. Currently, the database contains 2016 samples from 492 ChIP-seq and ChIP-chip experiments, representing a total of 170 proteins and 11 069 914 protein-DNA interactions. A web server provides interface for database query. Protein-DNA binding intensities can be retrieved from individual samples for user-provided genomic regions. The retrieved intensities can be used to cluster samples and genomic regions to facilitate exploration of combinatorial patterns, cell-type dependencies, and cross-sample variability of protein-DNA interactions. AVAILABILITY: http://jilab.biostat.jhsph.edu/database/cgi-bin/hmChIP.pl.