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991.
Yong‐Bo Hu Ru‐Jing Ren Yong‐Fang Zhang Yue Huang Hai‐Lun Cui Chao Ma Wen‐Ying Qiu Hao Wang Pei‐Jing Cui Hong‐Zhuan Chen Gang Wang 《Aging cell》2019,18(5)
Rho‐associated coiled‐coil kinase 1 (ROCK1) is proposed to be implicated in Aβ suppression; however, the role for ROCK1 in amyloidogenic metabolism of amyloid precursor protein (APP) to produce Aβ was unknown. In the present study, we showed that ROCK1 kinase activity and its APP binding were enhanced in AD brain, resulting in increased β‐secretase cleavage of APP. Furthermore, we firstly confirmed that APP served as a substrate for ROCK1 and its major phosphorylation site was located at Ser655. The increased level of APP Ser655 phosphorylation was observed in the brain of APP/PS1 mice and AD patients compared to controls. Moreover, blockade of APP Ser655 phosphorylation, or inhibition of ROCK1 activity with either shRNA knockdown or Y‐27632, ameliorated amyloid pathology and improved learning and memory in APP/PS1 mice. These findings suggest that activated ROCK1 targets APP Ser655 phosphorylation, which promotes amyloid processing and pathology. Inhibition of ROCK1 could be a potential therapeutic approach for AD. 相似文献
992.
Xiao‐Xi Pan Cheng‐Chao Ruan Xiu‐Ying Liu Ling‐Ran Kong Yu Ma Qi‐Hong Wu Hai‐Qing Li Yan‐Jun Sun An‐Qing Chen Qiang Zhao Fang Wu Xiu‐Jie Wang Ji‐Guang Wang Ding‐Liang Zhu Ping‐Jin Gao 《Aging cell》2019,18(4)
Aging is an independent risk factor for vascular diseases. Perivascular adipose tissue (PVAT), an active component of the vasculature, contributes to vascular dysfunction during aging. Identification of underlying cell types and their changes during aging may provide meaningful insights regarding the clinical relevance of aging‐related vascular diseases. Here, we take advantage of single‐cell RNA sequence to characterize the resident stromal cells in the PVAT (PVASCs) and identified different clusters between young and aged PVASCs. Bioinformatics analysis revealed decreased endothelial and brown adipogenic differentiation capacities of PVASCs during aging, which contributed to neointimal hyperplasia after perivascular delivery to ligated carotid arteries. Mechanistically, in vitro and in vivo studies both suggested that aging‐induced loss of peroxisome proliferator‐activated receptor‐γ coactivator‐1 α (PGC1α) was a key regulator of decreased brown adipogenic differentiation in senescent PVASCs. We further demonstrated the existence of human PVASCs (hPVASCs) and overexpression of PGC1α improved hPVASC delivery‐induced vascular remodeling. Our finding emphasizes that differentiation capacities of PVASCs alter during aging and loss of PGC1α in aged PVASCs contributes to vascular remodeling via decreased brown adipogenic differentiation. 相似文献
993.
Shuai Yu Chunping Miao Hong Song Yanqing Huang Wei Chen 《International journal of phytoremediation》2019,21(7):643-651
AbstractIncreased nitrogen and phosphorus pollution causes eutrophication in water bodies. Using aquatic plants to remove nutrients from water is an attractive phytoremediation. It is a cost-effective, environment-friendly, and efficient way that reduces water body eutrophication by the plant. It is important to choose suitable macrophytes to remove excess N and P under different nutrient conditions. In this study, six macrophyte species (Polygonum orientale, Juncus effuses, Iris pseudocorus, Phragmites australis, Iris sanguinea, Typha orientalis) were tested. Simulation experiment was conducted under five N and P levels. The removal rate, relative growth rate, and the dynamic nutrition concentration of cultivated solution were investigated. Of all the treatment, a 23–95% reduction in N removal and a 29–92% reduction in P removal were recorded. The results showed I. sanguinea is a promising species to treat various eutrophic waters and the other five species can be used specifically to treat certain types of water. The data provided a theoretical guidance to plant species selection for phytoremediation of polluted water bodies for the purpose of water quality improvement around the different reservoir in northern China. 相似文献
994.
The residues of phenothiazines and benzodiazepines in foods of animal origin are dangerous to consumers. For inspection of their abuses, this study for the first time reported on the use of a chemiluminescence array sensor for the simultaneous determination of four phenothiazines and five benzodiazepines in pig urine. Two molecularly imprinted polymers were coated in different wells of a conventional 96‐well microtiter plate as the recognition reagents. After sample loading, the absorbed analytes were initiated directly by using an imidazole enhanced bis(2,4,6‐trichlorophenyl)oxalate–hydrogen peroxide system to emit light. The assay process consisted of only one sample‐loading step prior to data acquisition, so one test was finished within 10 min. The limits of detection for the nine drugs in the pig urine were in a range of 0.1 to 0.6 pg/mL, and the recoveries from the fortified blank urine samples were in a range of 80.3 to 95%. Furthermore, the sensor could be reused six times. Therefore, this sensor could be used as a simple, rapid, sensitive and reusable tool for routine screening for residues of phenothiazines and benzodiazepines in pig urine. 相似文献
995.
996.
Wei Wang Kun Lv Ji‐Rui Wang Jing Zhou Jian‐Qiang Gu Guo‐Xin Zhou Zhi‐Hong Xu 《Entomological Research》2019,49(3):113-122
In the present study, partial sequences of the mitochondrial cytochrome oxidase subunit I (COI) gene of 22 island populations of the springtail Homidia socia in the Thousand Island Lake were sequenced. Across all sequences, 37 haplotypes were identified for the 510‐bp mitochondrial (mt) DNA COI gene. Haplotype 2 was the most common, and was distributed in the most of the 22 island populations. Haplotype diversity ranged from 0.065 to 0.733, and the total genetic diversity was 0.56216. The genetic characteristics of the 22 island populations were analyzed using the fixation index and gene flow, with values of 0.00043–0.94900 and 0.02703–703.72540, respectively. Comparison between (island area and isolations) with population genetic diversity revealed that there were no significant correlations between them, except for a significant correlation between the number of haplotypes and island area. Mantel tests showed that there was no significant correlation between geographic distance and genetic distance among various groups. All the results indicated that there were no obvious relationships between island characteristics and the genetic diversity of the springtails. We consider that the low dispersal capacity of springtails and the island patches surrounded by water in the Thousand Island Lake are the major factors affecting the genetic diversity of H. socia. 相似文献
997.
Jill K. Olofsson Isabel Cantera Cline Van de Paer Cynthia Hong‐Wa Loubab Zedane Luke T. Dunning Adriana Alberti Pascal‐Antoine Christin Guillaume Besnard 《Molecular ecology resources》2019,19(4):877-892
Species trees have traditionally been inferred from a few selected markers, and genome‐wide investigations remain largely restricted to model organisms or small groups of species for which sampling of fresh material is available, leaving out most of the existing and historical species diversity. The genomes of an increasing number of species, including specimens extracted from natural history collections, are being sequenced at low depth. While these data sets are widely used to analyse organelle genomes, the nuclear fraction is generally ignored. Here we evaluate different reference‐based methods to infer phylogenies of large taxonomic groups from such data sets. Using the example of the Oleeae tribe, a worldwide‐distributed group, we build phylogenies based on single nucleotide polymorphisms (SNPs) obtained using two reference genomes (the olive and ash trees). The inferred phylogenies are overall congruent, yet present differences that might reflect the effect of distance to the reference on the amount of missing data. To limit this issue, genome complexity was reduced by using pairs of orthologous coding sequences as the reference, thus allowing us to combine SNPs obtained using two distinct references. Concatenated and coalescence trees based on these combined SNPs suggest events of incomplete lineage sorting and/or hybridization during the diversification of this large phylogenetic group. Our results show that genome‐wide phylogenetic trees can be inferred from low‐depth sequence data sets for eukaryote groups with complex genomes, and histories of reticulate evolution. This opens new avenues for large‐scale phylogenomics and biogeographical analyses covering both the extant and the historical diversity stored in museum collections. 相似文献
998.
999.
Ines Pires da Silva Isabella C. Glitza Lauren E. Haydu Romany Johnpulle Patricia D. Banks George D. Grass Simone M. A. Goldinger Jessica L. Smith Ashlyn S. Everett Peter Koelblinger Rachel Roberts‐Thomson Michael Millward Victoria G. Atkinson Alexander Guminski Rony Kapoor Robert M. Conry Matteo S. Carlino Wei Wang Mark J. Shackleton Zeynep Eroglu Serigne Lo Angela M. Hong Georgina V. Long Douglas B. Johnson Alexander M. Menzies 《Pigment cell & melanoma research》2019,32(4):553-563
1000.
Zhao Jianlin Peng Wei Ran Yuxin Ge Huisheng Zhang Chen Zou Hong Ding Yubin Qi Hongbo 《Journal of physiology and biochemistry》2019,75(4):475-487
Journal of Physiology and Biochemistry - Preeclampsia (PE) is a hypertensive disease associated with increased endothelial cell dysfunction caused by systemic oxidative stress. Alpha-actinin-4... 相似文献