全文获取类型
收费全文 | 25426篇 |
免费 | 2284篇 |
国内免费 | 2642篇 |
出版年
2024年 | 40篇 |
2023年 | 227篇 |
2022年 | 504篇 |
2021年 | 910篇 |
2020年 | 722篇 |
2019年 | 836篇 |
2018年 | 829篇 |
2017年 | 679篇 |
2016年 | 915篇 |
2015年 | 1434篇 |
2014年 | 1701篇 |
2013年 | 1833篇 |
2012年 | 2336篇 |
2011年 | 2036篇 |
2010年 | 1432篇 |
2009年 | 1313篇 |
2008年 | 1597篇 |
2007年 | 1446篇 |
2006年 | 1361篇 |
2005年 | 1254篇 |
2004年 | 1157篇 |
2003年 | 1051篇 |
2002年 | 888篇 |
2001年 | 638篇 |
2000年 | 531篇 |
1999年 | 497篇 |
1998年 | 316篇 |
1997年 | 237篇 |
1996年 | 218篇 |
1995年 | 196篇 |
1994年 | 167篇 |
1993年 | 128篇 |
1992年 | 144篇 |
1991年 | 121篇 |
1990年 | 97篇 |
1989年 | 94篇 |
1988年 | 63篇 |
1987年 | 70篇 |
1986年 | 57篇 |
1985年 | 31篇 |
1984年 | 20篇 |
1983年 | 38篇 |
1982年 | 19篇 |
1981年 | 20篇 |
1979年 | 18篇 |
1978年 | 14篇 |
1977年 | 13篇 |
1975年 | 10篇 |
1972年 | 12篇 |
1970年 | 11篇 |
排序方式: 共有10000条查询结果,搜索用时 953 毫秒
211.
新疆10种沙生植物旱生结构的解剖学研究 总被引:17,自引:1,他引:16
新疆10种沙生植物的形态解剖研究表明,它们为适应沙生环境形态结构发生变化。叶器官的形态呈三种类型:叶片退化成膜质或鳞片状,而由同化枝执行光合功能;叶片上下都具栅栏组织,表皮角质膜厚,表皮毛发达,气孔下陷,输导组织和机械组织都发达;叶片肉质化,叶肉组织不分化,贮水组织发达而输导组织不发达。轴器官中厚壁组织发达,围绕维管组织,维管组织内部也有发达的厚壁组织。根中普遍具有周皮,一些植物存在异常的维管组织,部分植物还具有粘液细胞或结晶。沙生植物形成各种旱生结构,以不同的方式适应沙生环境。 相似文献
212.
213.
我国自然保护区的生态旅游开发 总被引:42,自引:0,他引:42
我国自然保护区的生态旅游开发袁兴中,刘红,高天刚(山东曲阜师范大学273165)DevelopmentofEcotourisminNaturalReservesofChina¥YuanXingzhong;LiuHong;GaoTiangang(Quf... 相似文献
214.
大鼠背部20%体表面积接触沸水20s,形成Ⅲ度烫伤后,延髓孤束核推挽灌流液中β-内啡肽免疫活性物质的含量显著升高,在烫后1、4h两次达到峰值。烫后立即向弧束核微量注射0.4μlβ-内啡肽抗血清(滴度1:30000),可在一定程度上改善心功能指标,延缓血压和心率的下降,但未能延长动物的存活时间。提示孤束核的β-内啡肽在烫伤休克的病理过程中起作用,其含量的过量升高有抑制心血管功能的作用,从而不利于烫伤休克的恢复。 相似文献
215.
An anticomplementary agent, K-76 monocarboxylic acid: its site and mechanism of inhibition of the complement activation cascade. 总被引:11,自引:0,他引:11
K Hong T Kinoshita W Miyazaki T Izawa K Inoue 《Journal of immunology (Baltimore, Md. : 1950)》1979,122(6):2418-2423
A monocarboxylic acid derivative (K-76 COOH) of K-76, purified from the culture filtrate of Stachybotrys complement I nov. sp. K-76, inhibits complement (C) activity. Its inhibitory action is mainly on C5 step. It strongly inhibits the generation of EAC1,4b,2a,3b,5b from C5 and EAC1,4b,2a,3b, and accelerates the decay of EAC1,4b,2a,3b,5b. It also causes some inhibition of the reactions of the reactions of C2,C3,C6,C7 and C9 with their respective preceding intermediate cells. It has no effect on the generation of EAC1,4b from C4 and EAC1, or of EAC-8 from C8 and EAC-7, and apparently increases the generation of EAC1,4b from C1 and EAC4b probably by inhibiting transfer or turnover of C1. It does not affect the rate of decay of EAC1,4b,2a or the T max of generation of EAC1,4b,2a, and it inhibits immune adherence only at high concentration. K-76 COOH also strongly inhibits hemolysis through the alternative pathway of C activation by cobra venom factor, but it does not seem to inhibit the early steps of the alternative pathway, because it has little affect on the consumption of C3 or the conversion of beta 1C to beta 1A on treatment of C serum with zymosan. K-76 COOH probably combines with C5 molecules, forming the inactive complexes, or it causes the structural alteration of C5. 相似文献
216.
An experiment is described in which the effects of age, previous infection, pregnancy and lactation on some reactions of cattle to infection with Ostertagia ostertagi were studied. It was found that an acquired resistance to the establishment of worms developed more rapidly in 20-month-old heifers than in calves, that it was unaffected by pregnancy of the host but that it was largely lost by heifers in early lactation. The rate at which populations were turned over, i.e. the mean life-span of worms through the late 4th and 5th stages was unaffected by the factors studied. Although, in the conditions of the experiment, development of the worms was not arrested in susceptible calves, both age of the host and its previous experience of infection were significant causes of arrest, and in previously infected 20-month-old cattle 86% of the worms of a challenge infection were arrested. Pregnancy did not affect the proportion of worms that was arrested but in lactating heifers only marginally more worms were arrested than in calves. Worms that were not arrested grew more rapidly in calves and in lactating heifers than in empty heifers or those in mid-pregnancy. 相似文献
217.
Electron microscopic and biochemical investigations of developing embryonic mustard cotyledons provided no evidence for the widely accepted hypothesis that oleosomes of fat-storing tissues originate from the endoplasmic reticulum and are surrounded by a unit- or half-unit membrane. In contrast, it was found that the first lipid droplets appear (about 12–14 d after pollination) in the ground cytoplasm near the surface of plastids. Subsequently these nascent lipid droplets, which lack any detectable boundary structure at this stage, become encircled by a cisterna of rough endoplasmic reticulum. At the same time an osmiophilic coat of about 3 nm thickness becomes detectable at the lipid/water interface. In the cotyledon cells of germinating seedlings a centrifugally moving front of fat degradation moves from the central vacuoles(s) towards the cell periphery, leaving behind collapsed coats of oleosomes which are depleted of their lipid contents (saccules). Although saccules appear tripartite in cross section, they are structurally different from endoplasmic reticulum membranes. The oleosome coats can be isolated from oleosome preparations by extracting lipids with organic solvents. The coat material is insoluble in detergents like Triton X-100 or deoxycholate and shows a tripartite, lamellar structure (similar to collapsed saccules) under the electron microscope. Upon dissolution with dodecylsulfate, polyacrylamide gel electrophoresis revealed a polypeptide composition (9 major bands) which is qualitatively different from that of the endoplasmic reticulum membrane. Also the buoyant densities of defatted oleosome coats and defatted endoplasmic reticulum membranes are very different. It is concluded that oleosome lipids accumulate in the ground cytoplasm and are bounded by a lamellar structure originating de novo from proteinaceous elements synthesized by specific regions of the endoplasmic reticulum.Abbreviation ER
endoplasmic reticulum 相似文献
218.
本文通过对枯草芽孢杆菌BR151衍生株与北京棒杆菌1134衍生株的赖氨酸高产融合子Q4413株的形态学、生理生化特性等方面的研究,揭示了融合子与双亲株在这些方面的差异,为Q4413株确系双亲株的重组子或新的融合子增加了佐证. 相似文献
219.
Phosphorylation of the AfsR product, a global regulatory protein for secondary-metabolite formation in Streptomyces coelicolor A3(2). 总被引:3,自引:1,他引:2
下载免费PDF全文
![点击此处可从《Journal of bacteriology》网站下载免费的PDF全文](/ch/ext_images/free.gif)
The AfsR protein is essential for the biosynthesis at the wild-type level of A-factor, actinorhodin, and undecylprodigiosin in Streptomyces coelicolor A3(2) and Streptomyces lividans. Because overexpression of the afsR gene caused some deleterious effect on these strains, a multicopy plasmid carrying the whole afsR gene was introduced into Streptomyces griseus, from which a crude cell lysate was prepared as a protein source. The AfsR protein was purified to homogeneity from the cytoplasmic fraction through several steps of chromatography, including affinity column chromatography with ATP-agarose and use of anti-AfsR antibody for its detection. The molecular weight of AfsR was estimated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and by gel filtration to be 105,300, which is in good agreement with that deduced from the nucleotide sequence of afsR. The purified AfsR protein was found to be phosphorylated through the transfer of the gamma-phosphate group of ATP in the presence of the cell extracts of S. coelicolor A3(2) and S. lividans. This phosphorylation proceeded very rapidly, and no competition was observed with CTP, GTP, UTP, or cyclic AMP. In the cell extract of S. griseus, no activity phosphorylating the AfsR protein was detected, suggesting that this activity is not generally present in Streptomyces spp. but is specific to certain species. It is conceivable that the extent of phosphorylation of the AfsR protein modulates its regulatory activity which, in turn, regulates expression of some target gene(s) involved in the secondary-metabolite formation in S. coelicolor A3(2). 相似文献
220.
T Ohashi C M Hong S Weiler J M Tomich J M Aerts J M Tager J A Barranger 《The Journal of biological chemistry》1991,266(6):3661-3667
Human cDNA was mutagenized to duplicate six naturally occurring mutations in the gene for glucocere-brosidase. The mutant genes were expressed in NIH 3T3 cells. The abnormal human enzymes were purified by immunoaffinity chromatography and characterized. The Asn370----Ser mutant protein differed from normal enzyme in its inhibition by both conduritol B epoxide and glucosphingosine demonstrating that the 370 mutant enzyme has an abnormal catalytic site. In addition, the 370 mutant enzyme is less activated by saposin C, but more stimulated by phosphatidylserine than the wild type enzyme. The Arg463----Cys mutant protein was normal with respect to conduritol B epoxide and glucosphingosine inhibition, but was less activated by both saposin C and phosphatidylserine. The Arg120----Gln mutant protein was catalytically inactive. The Leu444----Pro, the pseudopattern, and the Pro415----Arg mutants appear to have reduced amounts of enzyme protein in cells. The studies demonstrated that mutations in the gene for glucocerebrosidase have different effects on the catalytic activity and stability of the enzyme. 相似文献