人α1/158Ⅴ型(α1c型)干扰素基因在大肠杆菌中的表达及其产物的初步纯化

采用DNA聚合酶链反应(PCR)技术,将本实验室从中国人胎肝细胞染色体DNA中发现和分离的IFN-α1/158V基因的原始克隆,改造成适于进行非融合蛋白原核表达的结构形式,并在大肠杆菌中获得高效表达。测得重组IFN-α1/158V的抗病毒活性为1.9×10~7单位/升菌液。随后又采用以单克隆抗体亲和层析为主的纯化流程对表达产物进行初步纯化,获得了在SDS-聚丙烯酰胺凝胶电泳上呈现单一条带的纯化产物。     

越冬期间香蕉果穗套袋的防寒效果及其对产量的影响

冬季香蕉果穗用兰色聚乙烯薄膜套袋之后,可提高袋内温度1～5℃,果实低温伤害卑只有1％左右,而未经套袋的达23％～35％,果指长度和径围增加率分别达12％和9％左右,果实产量比对照增加8％～16％。试验结果表明,兰色聚乙烯薄膜套袋是越冬期间香蕉防寒保果和增产的一项有效措施。     

Further investigation on nuclear transplantation in different orders of teleost: the combination of the nucleus of Tilapia (Oreochromis nilotica) and the cytoplasm of Loach (Paramisgurnus dabryanus).

The nucleus of a blastula cell from Tilapia (Oreochromis nilotica, family Cichlidae, order Perciformes) was transplanted into an enucleated egg of Loach (Paramisgurnus dabryanus, family Cobitidae, order Cypriniformes). From among 3747 nucleo-cytoplasmic hybrid (NCH) eggs two NCH larval fish (0.05%) were obtained; one died on the 6th day and the other died on the 12th day after the operation. Morphological examinations showed that both NCH larval fish had developed normally with an opened mouth except they could not take food after complete utilization of their egg yolk on the 5th day of development. The possible mechanisms for obtaining such inter-order NCH larval fish are discussed. This is the first report indicating that inter-order NCH larval fish can be obtained in spite of their evolutionary divergence.     

American anthropologists looking through Taiwanese culture

Conclusion Anthropologists have too often supported — in their words, in their presences, and in their deeds — the paternalistic claims of rulers suppressing the very cultures they want to study. Much anthropological work legitimates substitution of the language of Beijing for Hakka and Holo, justifies ignoring Taiwanese culture, and subordinates consideration of its specific features to writing about Chinese civilization, just as Taiwanese are economically and politically subordinated to the ethnic minority dictatorship which still rules Taiwan under the fiction of being the government of China. The China that Arthur Wolf and others serve in return for support of their research from what purports to be the Republic of China is an egregious, but unfortunately not unique, example.Stephen O. Murray is with El Instituto Obregón, San Francisco, California. Keelung Hong is with the Cancer Research Institute, University of California, San Francisco.As is normal in anthropology, except for Taiwan, native terms are romanized in the native language (in the first author's recording of the second author's Daidiong idiolect, without indication of the extremely intricate tone differentia), not in the official language of the ethnic oligarchy ruling Taiwan (Beijinghua). Mainland place names are rendered in pinyin. We have followed the convention of treating Chinese and Taiwanese personal names as two words, because books are catalogued with such names. If personal names are considered as two words (an imposition of the hoary dogma that words in Sino-Tibetan languages are monosyllabic), it seems to us that both words should be capitalized. For Taiwanese placenames, we have not separated syllables by dashes or by spaces.     

Plant-microbial interaction under gnotobiotic conditions: A scanning electron microscope study

Inoculation of canola seeds withPseudomonas putida GR12-2 stimulates root elongation under gnotobiotic conditions. Transformation ofP. putida GR12-2 with the broad-host-range plasmid pGSS15 abolishes the enhancement of root elongation. With scanning electron microscopy it was found that both transformed and nontransformedP. putida GR12-2 are capable of binding to canola seed coats. In addition, it was observed that 4 days after the initial inoculation the roots of bothP. putida GR12-2- and GR12-2/pGSS15-treated seedlings were free of adhering bacteria despite the fact that it was subsequently shown that both bacterial strains are capable of binding to roots. Thus, adhesion to roots is not necessary for the initial phase of enhanced root elongation that is induced byP. putida GR12-2 under gnotobiotic conditions.