全文获取类型
收费全文 | 162篇 |
免费 | 25篇 |
国内免费 | 1篇 |
专业分类
188篇 |
出版年
2021年 | 1篇 |
2018年 | 3篇 |
2017年 | 1篇 |
2016年 | 4篇 |
2015年 | 10篇 |
2014年 | 12篇 |
2013年 | 8篇 |
2012年 | 10篇 |
2011年 | 6篇 |
2010年 | 5篇 |
2009年 | 4篇 |
2008年 | 8篇 |
2007年 | 16篇 |
2006年 | 7篇 |
2005年 | 10篇 |
2004年 | 8篇 |
2003年 | 7篇 |
2002年 | 6篇 |
2001年 | 12篇 |
2000年 | 7篇 |
1999年 | 2篇 |
1998年 | 2篇 |
1997年 | 3篇 |
1996年 | 3篇 |
1995年 | 1篇 |
1994年 | 3篇 |
1993年 | 2篇 |
1992年 | 3篇 |
1991年 | 2篇 |
1990年 | 6篇 |
1989年 | 1篇 |
1988年 | 1篇 |
1987年 | 1篇 |
1984年 | 2篇 |
1982年 | 1篇 |
1978年 | 1篇 |
1974年 | 3篇 |
1973年 | 3篇 |
1972年 | 1篇 |
1926年 | 1篇 |
1910年 | 1篇 |
排序方式: 共有188条查询结果,搜索用时 0 毫秒
31.
32.
Detergents are commonly used for the extraction of hydrophobic proteins and must be removed for sensitive detection of peptides by mass spectrometry. We demonstrate that ethyl acetate is able to extract octylglycoside from a protease digest without loss of peptides or interference with the peptide mass spectral profile. Ethyl acetate extraction was also found to reduce interference by sodium dodecyl sulfate, Nonidet P-40, or Triton X-100 in the mass spectrometry analysis. 相似文献
33.
S Suthutvoravut C J Hogue B Guyer M Anderka M W Oberle 《Journal of biosocial science》1989,21(4):443-451
In birth certificate data for Massachusetts resident births from 1978 to 1982, 12-27% of births purportedly under 31 weeks of gestation were probably misclassified, i.e. had birthweight greater than or equal to 2500 g. Correcting for maldistribution of births removed 34% and 23%, respectively, of black and white births with reported gestational ages less than 36 weeks but with implausible weights. Percentages of unknown and incomplete reports of last menstrual period were also significantly higher for blacks. After adjustment, preterm black infants weighed less than whites at each gestational age. The proportion of infants less than 2500 g born at term (greater than or equal to 37 weeks gestation) was higher (although not significantly) among blacks. These findings are consistent with hypotheses that low socioeconomic status negatively affects the rate of intrauterine growth. 相似文献
34.
Gunaratne J Goh MX Swa HL Lee FY Sanford E Wong LM Hogue KA Blackstock WP Okumura K 《The Journal of biological chemistry》2011,286(20):18093-18103
The tumor suppressor PTEN (phosphatase and tensin homologue) negatively regulates the PI3K pathway through its lipid phosphatase activity and is one of the most commonly lost tumor suppressors in human cancers. Though the tumor suppressive function involves the lipid phosphatase-dependent and -independent activities of PTEN, the mechanism leading to the phosphatase-independent function of PTEN is understood poorly. Some PTEN mutants have lipid phosphatase activity but fail to suppress cell growth. Here, we use a cancer-associated mutant, G20E, to gain insight into the phosphatase-independent function of PTEN by investigating protein-protein interactions using MS-based stable isotope labeling by amino acids in cell culture (SILAC). A strategy named parallel affinity purification (PAP) and SILAC has been developed to prioritize interactors and to compare the interactions between wild-type and G20E PTEN. Clustering of the prioritized interactors acquired by the PAP-SILAC approach shows three distinct clusters: 1) wild-type-specific interactors, 2) interactors unique to the G20E mutant, and 3) proteins common to wild-type and mutant. These interactors are involved mainly in cell migration and apoptosis pathways. We further demonstrate that the wild-type-specific interactor, NUDTL16L1, is required for the regulatory function of wild-type PTEN in cell migration. These findings contribute to a better understanding of the mechanisms of the phosphatase-dependent and -independent functions of PTEN. 相似文献
35.
ABSTRACTThe Caribbean blepharicerid fauna comprises three described species: Paltostoma palominoi Hogue and Garces, 1990, found in eastern Cuba; P. agyrocincta Curran, 1927, widespread in Puerto Rico; and P. schineri Williston, 1896, which occupies most major islands of the Lesser Antilles. In this paper, we describe P. portoricensis Hogue and Courtney, sp. n., a second species from Puerto Rico. We also provide keys to adults, pupae, and larvae of known Caribbean species, re-describe P. argyrocincta and P. schineri, and present a brief discussion of the bionomics and phylogenetic affinities of the net-winged midge fauna. 相似文献
36.
本文报道海拔3417m和4280m地区世居藏族和移居汉族青少年运动状态下心肺功能的对比研究。结果显示:3417m和4280m世居藏族的最大氧耗量、无氧阈值及最大心输出量都明显大于汉族,血氧饱和度(Sao2)随运动负荷的增加而降低。海拔3417m藏、汉族的△Sao2分别为7.46%和10.03%,4280m处为8.57%和13.75%,最大心率随海拔升高而下降。研究提示,藏族青少年有较高的最大有氧能力,反映了他们对低氧环境的适应优势。 相似文献
37.
Coronavirus envelope (E) proteins are small (approximately 75- to 110-amino-acid) membrane proteins that have a short hydrophilic amino terminus, a relatively long hydrophobic membrane domain, and a long hydrophilic carboxy-terminal domain. The protein is a minor virion structural component that plays an important, not fully understood role in virus production. It was recently demonstrated that the protein forms ion channels. We investigated the importance of the hydrophobic domain of the mouse hepatitis coronavirus (MHV) A59 E protein. Alanine scanning insertion mutagenesis was used to examine the effect of disruption of the domain on virus production in the context of the virus genome by using a MHV A59 infectious clone. Mutant viruses exhibited smaller plaque phenotypes, and virus production was significantly crippled. Analysis of recovered viruses suggested that the structure of the presumed alpha-helical structure and positioning of polar hydrophilic residues within the predicted transmembrane domain are important for virus production. Generation of viruses with restored wild-type helical pitch resulted in increased virus production, but some exhibited decreased virus release. Viruses with the restored helical pitch were more sensitive to treatment with the ion channel inhibitor hexamethylene amiloride than were the more crippled parental viruses with the single alanine insertions, suggesting that disruption of the transmembrane domain affects the functional activity of the protein. Overall the results indicate that the transmembrane domain plays a crucial role during biogenesis of virions. 相似文献
38.
Shlomo Grossman John Cobley P.K. Hogue Edna B. Kearney Thomas P. Singer 《Archives of biochemistry and biophysics》1973,158(2):744-753
The disappearance of succinate dehydrogenase activity and of protein-bound histidyl flavin were studied in aerobic yeast cells incubated with high glucose concentrations. The decay of succinate dehydrogenase activity, covalently bound flavin, and of respiration is prevented by cycloheximide but not by chloramphenicol. During this decay there is a large increase in mitochondrial phospholipase D activity; the appearance of this enzyme is also prevented by cycloheximide. It seems possible, therefore, that the formation of phospholipase D may be important in triggering the disappearance of covalently bound flavin, succinate dehydrogenase, and of other mitochondrial enzymes during glucose repression of aerobic yeast cells. 相似文献
39.
M. J. Hogue 《Biotechnic & histochemistry》1926,1(1):35-36
The use of janus green to stain mitochondria has long been known. While using it to study the mitochondria in Trichomonas buccalis it was found to stain the flagella also. This is an easy and quick method of determining the number of anterior flagella of the trichomonads and so of distinguishing the trichomonads from the pentatrichomonads in intestinal infections. This stain proved so useful as a flagella stain that it was applied to numerous protozoa with interesting results. 相似文献
40.
Radek Bukowski Nellie I. Hansen Marian Willinger Uma M. Reddy Corette B. Parker Halit Pinar Robert M. Silver Donald J. Dudley Barbara J. Stoll George R. Saade Matthew A. Koch Carol J. Rowland Hogue Michael W. Varner Deborah L. Conway Donald Coustan Robert L. Goldenberg for the Eunice Kennedy Shriver National Institute of Child Health Human Development Stillbirth Collaborative Research Network 《PLoS medicine》2014,11(4)