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11.
A total of 548 spiders (Gnaphosidae, Clubionidae, Thomisidae, Philodromidae, Salticidae, Lycosidae, Pisauridae, Linyphiidae) from three sand dune grassland sites were tested serologically for feeding on the grasshoppers, Chorthippus brunneus (Thunberg) and Myrmeleotettix maculatus (Thunberg). Lycosidae were the most commonly tested species and gave the greatest proportion of positive tests. Laboratory observations suggest that predation in the field was predominantly on first instar grasshoppers.
Résumé Afin d'améliorer la connaissance de l'importance des prédateurs dans la biologie des populations de criquets (Orthoptères; Acrididae), les araignées de trois pelouses sur dunes de sable ont été examinées sérologiquement pour estimer leur consommation des populations sympatriques de Chorthippus brunneus Thunberg et Myrmeleotettix maculatus Thunberg. Au total, 548 araignées (Gnaphosidae, Clubionidae, Thomisidae, Philodromidae, Salticidae, Lycosidae, Pisauridae, Linyphiidae) ont été récoltées à la main ou par piège pendant la période d'éclosion des ufs de criquets. Les Lycosidae ont été le plus fréquemment observées (90,5% de toutes les récoltes) et ont donné la plus forte proportion d'individus positifs (jusqu'à 32,3%). Les expériences d'alimentation en laboratoire suggèrent que, dans la nature, les Lycosidae sont les plus actives contre les criquets du premier stade.相似文献
12.
Biochemical characterization of the Drosophila dpp protein, a member of the transforming growth factor beta family of growth factors. 下载免费PDF全文
G E Panganiban K E Rashka M D Neitzel F M Hoffmann 《Molecular and cellular biology》1990,10(6):2669-2677
The decapentaplegic (dpp) gene of Drosophila melanogaster is required for pattern formation in the embryo and for viability of the epithelial cells in the imaginal disks. The dpp protein product predicted from the DNA sequence is similar to members of a family of growth factors that includes transforming growth factor beta (TGF-beta). We have produced polyclonal antibodies to a recombinant dpp protein made in bacteria and used a metallothionein promoter to express a dpp cDNA in Drosophila S2 cells. Similar to other proteins in the TGF-beta family, the dpp protein produced by the Drosophila cells was proteolytically cleaved, and both portions of the protein were secreted from the cells. The amino-terminal 47-kilodalton (kDa) peptide was found in the medium and in the proteins adhering to the plastic petri dish. The carboxy-terminal peptide, the region with sequence similarity to the active ligand portion of TGF-beta, was found extracellularly as a 30-kDa homodimer. Most of the 30-kDa homodimer was in the S2 cell protein adsorbed onto the surface of the plastic dish. The dpp protein could be released into solution by increased salt concentration and nonionic detergent. Under these conditions, the amino-terminal and carboxy-terminal portions of dpp were not associated in a stable complex. 相似文献
13.
A new repetitive protein from Xenopus laevis skin highly homologous to pancreatic spasmolytic polypeptide 总被引:7,自引:0,他引:7
W Hoffmann 《The Journal of biological chemistry》1988,263(16):7686-7690
A cDNA sequence has been used to derive the precursor structure of a highly repetitive protein in Xenopus laevis skin. From the sequence of a whole family of secretory proteins can be predicted containing a classical hydrophobic signal sequence at the NH2-terminal end of the precursor. The proteins contain four domains with high homology to porcine pancreatic spasmolytic polypeptide. These four cysteine-rich, presumably physiologically active domains are separated in the molecule by a repetitive element, locating two such domains to the NH2 terminus of the precursor protein and the remaining two to the COOH-terminal end. The separating spacer consists of very unusual, precise, threonine and proline-rich repeats containing 9 residues which could be targets for extensive O-glycosylation. Additionally, processing at two pairs of basic residues is suggested to liberate two polypeptides ("spasmolysins") and "spasmolysin-glycoprotein." 相似文献
14.
The Ehrlich ascites tumor cell has been used as a model of an unspecialized mammalian cell, in an attempt to disclose the mechanisms involved in the regulation of cellular water and salt content. In hypotonic medium Ehrlich cells initially swell as nearly perfect osmometers, but subsequently recover their volume within about 10 min with an associated net loss of KCl, amino acids, taurine and cell water. The net loss of KCl takes place mainly via separate, conductive K+ and Cl- transport pathways, and the net loss of taurine through a passive leak pathway. Ca2+ and calmodulin appear to be involved in the activation of the K+ and Cl- channels, as well as the taurine leak pathway. In hypertonic medium Ehrlich cells initially shrink as osmometers, but subsequently recover their volume with an associated net uptake of KCl and water. In this case, the net uptake of KCl is the result of the activation of an electroneutral, Na+- and Cl- -dependent cotransport system with subsequent replacement of cellular Na+ by extracellular K+ via the Na+/K+ pump. In the present review we describe the ion and taurine transporting systems which have been identified in the plasma membrane of the Ehrlich ascites tumor cell. We have emphasized the selectivity of these transport pathways and their activation mechanisms. Finally, we propose a model for the activation of the conductive K+ and Cl- transport pathways in Ehrlich cells which includes Ca2+, leukotrienes, and inositol phosphate as intracellular second messengers. 相似文献
15.
Characterization and localization of cis-diamminedichloro-platinum(II) adducts on a purified oligonucleotide containing the codons 12 and 13 of H-ras proto-oncogene. 下载免费PDF全文
M J Pillaire G Villani J S Hoffmann A M Mazard M Defais 《Nucleic acids research》1992,20(24):6473-6479
The use of substrates containing well defined adducts at precise sites, is required to perform a careful analysis of the toxic and mutagenic potential of a lesion. As a first step in this direction the octamer 5'-d(CCGGCGGT), containing the sequence of the codons 12 d(GGC) and 13 d(GGT) of the human H-ras gene, was reacted with the antitumoral drug cis-diamminedichloroplatinum(II). The platinated products have been purified by HPLC. A first set of experiments, including enzymatic digestions with nuclease P1 followed by alkaline phosphatase and acid-catalysed hydrolysis, allowed us to determine which bases were engaged in the cis-DDP lesions. Our results indicate that only guanine residues were chelated with cisplatin to yield bifunctional adducts. Furthermore, by performing enzymatic digestions with phosphodiesterases, we have located the adducts with respect to the 5' end of the octamer. Among the purified and characterized platinated oligonucleotides, three present a particular interest, since we have shown here that the cis-d(GpG) adduct is precisely situated either at the d(GGC) or at the d(GGT) or at both sites of their sequence. 相似文献
16.
17.
Authors studied an incidence of myocardial infarction in women, specially aged below 50 years. Infarct localisation, complications during the treatment, coronary disease risk factors and mortality were estimated. In the most young women with myocardial infarction 2-3 risk factors were found, simultaneously specially cigarette smoking--all studied women aged below 50 years smoked cigarettes. Hospital course of myocardial infarction was as a rule not complicated. Mortality in young and in all observed women was however twice higher in comparison to control group of males. 相似文献
18.
M. Heinz R. Krattenmacher B. Hoffmann W. Clauss 《Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology》1991,161(4):363-370
Summary Transepithelial electrogenic Na+ transport (INa) was investigated in the coprodeum of 20-days-old chicken embryos in Ussing chambers. Short circuit current (Isc) and transepithelial resistance (Rt) were 14.7±4.8 A · cm-2 (n=12) and 0.53±0.09 k · cm-2 (n=12), respectively. INa was calculated from changes in Isc by substitution of mucosal Na+ by (N-methyl-d-glucamine) (NMDG). Isc inversed during Na+ removal, and INa was found to be 27.8±4.7 A · cm-2 (n=12). Amiloride (100 mol · l-1) inhibited only about 60% of INa. Analysis of Isc fluctuations revealed a Lorentzian component in the power density spectrum with a corner frequency of about 57 Hz. This component was not correlated to INa, and its origin is still unclear. Removal of mucosal Ca2+ increased INa about 2.5-fold due to an increase of the amiloride-insensitive component of INa in additionally investigated adult tissues. The results clearly show that this is due to a non-selective cation channel with an apparent order of selectivity Cs+>Na+=K+>Rb+>Li+. The Ca2+ concentration required to block 50% of the Isc was about 18 mol · l-1. The I
sc
Ca
could also be supressed by other divalent cations such as Mg2+ and Ba2+. Additionally, an INa-linked Lorentzian component occurred which dominated the control spectrum with a significantly higher corner frequency (about 88 Hz). The results indicate that Na+ absorption in the coprodeum of the chicken embryo is more complex than in adult hens. However, the Ca2+ sensitivity of INa is similar to comparable effects described for other epithelia. This possibly reflects the existence of two types of amiloride-insensitive apical cation channels as pathways for Na+ absorption, which may be involved to differing degrees in ontogenetic developments of nonselective channels to Na+-specific ion channels.Abbreviations
DPL
direct-linear-plot method
-
slope of the back-ground noise component
-
EGTA
ethylene glycol-bi(2-amino-ethylether)-N,N,N,N-tetraacetic acid
-
f
frequency
-
f
c
corner frequency of the Lorentzian noise component
-
G
t
transepithelial conductance
-
HEPES
N-hydroxyethylpiperazine-N-ethanesulfonic acid
-
I
sc
short-circuit current
-
I
Na
transepithelial sodium current
-
I
sc
Ca
Ca2+-sensitive short-circuit current
-
K
m
Ca
Michaelis-Menten constant for Ca2+
-
K
B
power density of the background noise component at f=1Hz
-
m
mucosal
-
NMDG
N-methyl-D-glucamine
-
R
t
transepithelial resistance
-
s
serosal
-
SEM
standard error of mean
-
S(f)
power density of the Lorentzian noise component
-
S
o
plateau value of the Lorentzian noise component 相似文献
19.
Identification of a fibroblast growth factor-binding protein in Drosophila melanogaster. 总被引:1,自引:0,他引:1 下载免费PDF全文
As assessed by competitive binding and protein-crosslinking experiments, Drosophila melanogaster cells possess basic fibroblast growth factor (bFGF)-specific binding proteins that are similar to FGF receptors on vertebrate cells in molecular weight and binding affinity; these D. melanogaster cells, however, have no detectable binding proteins for acidic fibroblast growth factor (aFGF). Consistent with the presence of bFGF-specific binding proteins, D. melanogaster cells degrade bFGF but not aFGF. These results indicate the conservation of heparin-binding growth factors and receptors between vertebrates and D. melanogaster. 相似文献
20.
Striking homology of the ''variable'' N-terminal as well as the ''conserved core'' domains of the mouse and human TATA-factors (TFIID). 总被引:18,自引:9,他引:9 下载免费PDF全文
T Tamura K Sumita I Fujino A Aoyama M Horikoshi A Hoffmann R G Roeder M Muramatsu K Mikoshiba 《Nucleic acids research》1991,19(14):3861-3865