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41.
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Flemming Jessen Bruce D. Cherksey Thomas Zeuthen Else K. Hoffmann 《The Journal of membrane biology》1989,108(2):139-151
Summary Furosemide-binding proteins were isolated from cholate-solubilized membranes of Ehrlich ascites tumor cells by affinity chromatography, using furosemide as ligand. Solubilized proteins retarded by the affinity material were eluted by furosemide. In reducing and denaturing gels, the major proteins eluted by furosemide were 100 and 45 kDa. In nonreducing, nondenaturing gels, homodimers of both polypeptides were found, whereas no oligomeric proteins containing both polypeptides were seen. It is concluded that the furosemide gel binds two distinct dimeric proteins. The isolated proteins were reconstituted into phospholipid vesicles and the K+ transport activity of these vesicles was assayed by measurement of86Rb+ uptake against a large opposing K+ gradient. The reconstituted system was found to contain a K+ transporting protein, which is sensitive to Ba2+ like the K+ channel previously demonstrated to be activated in intact cells after cell swelling. 相似文献
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1. | Under laboratory conditions complete development of H. sparsutum is shown within a temperature range of-0.7°C to +8°C. Constant temperatures above 10°C are lethal to the population. Larval growth (L1 to L5) is strictly temperature-dependent between-0.7 and +3.5°C, but slightly temperature compensated between +3.5 and +8°C. |
2. | Rate of egg production is highest at an average daily temperature of-0.7°C. |
3. | The sixth larval stage (L6) can be subdivided into a wandering and feeding period of about 40 days (at 7.3°C) and a following prepupal resting stage (PPR) with a high variability in duration, even at one and the same temperature, which ends with pupation. |
4. | Entrance into this resting stage seems to be independent of environmental changes and can occur at every time of the year. Termination of the PPR depends upon light stimuli received during the entire larval period. |
5. | Readiness for pupation exists when the photoperiod in the PPR exceeds that during hatching of first larva. A decreasing photoperiod during PPR triggers pupation in prepupae of various age. |
6. | The diapause stage seems to be a primary factor synchronizing the life cycle with seasonal changes in the environment. |
46.
S Benyhe G Hoffmann E Varga S Hosztafi G Toth A Borsodi M Wollemann 《Life sciences》1989,44(24):1847-1857
Oxymorphazone (at doses of 50-200 mg/kg) was found to be a relatively weak antinociceptive drug in intact frog (Rana esculenta) when acetic acid was used as pain stimulus. Frogs remained analgesic for at least 48 hrs following oxymorphazone (200 mg/kg) administration. The ligand increased the latency of wiping reflex in spinal frogs too. These effects were blocked by naloxone. In equilibrium binding studies (3H)oxymorphazone had high affinity to the opioid receptors of frog brain and spinal cord as well (apparent Kd values were 8.9 and 10.6 nM, respectively). Kinetic experiments show that only 25% of the bound (3H)oxymorphazone is readily dissociable. Preincubation of the membranes with labeled oxymorphazone results in a washing resistant inhibition of the opioid binding sites. At least 70% of the (3H)oxymorphazone specific binding is apparently irreversible after reaction at 5 nM ligand concentration, and this can be enhanced by a higher concentration of tritiated ligand. 相似文献
47.
Bernhard Hoffmann Isolde Nagel Wolfgang Clauss 《Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology》1990,160(4):381-388
Summary Regulation of the paracellular pathway in rabbit distal colon by the hormone aldosterone was investigated in vitro in Ussing chambers by means of transepithelial and microelectrode techniques. To evaluate the cellular and paracellular resistances an equivalent circuit analysis was used. For the analysis the apical membrane resistance was altered using the antibiotic nystatin. Under control conditions two groups of epithelia were found, each clearly dependent on the light: dark regime. Low-transporting epithelia (LT) were observed in the morning and high-transporting epithelia (HT) in the afternoon. Na+ transport was about 3-fold higher in HT than in LT epithelia. Incubating epithelia of both groups with 0.1 mol·1-1 aldosterone on the serosal side nearly doubled in LT epithelia the short circuit current and transepithelial voltage but the transepithelial resistance was not influenced. Maximal values were reached after 4–5 h of aldosterone treatment. In HT epithelia due to the effect of aldosterone all three transepithelial parameters remained constant over time. Evaluation of the paracellular resistance revealed a significant increase after aldosterone stimulation in both epithelial groups. This increase suggests that tight junctions might have been regulated by aldosterone. The hormonal effect on electrolyte transport was also dependent on the physiological state of the rabbit colon. Since net Na+ absorption in distal colon is, in addition to transcellular absorption capacity, also dependent on the permeability of the paracellular pathway, the regulation of tight junctions by aldosterone may be a potent mechanism for improving Na+ absorption during hormone-stimulated ion transport.Abbreviations
V
t
transepithelial potential difference (mV)
-
R
t
transepithelial resistance (·cm2)
-
G
t
transepithelial conductance (mS·cm-2)
- Isc
calculated short circuit current (A·cm-2)
-
V
a
apical membrane potential difference (mV)
-
V
bl
basolateral membrane potential difference (mV)
-
voltage divider ratio
-
R
a
apical membrane resistance (·cm2)
-
R
bl
basolateral membrane resistance (·cm2)
-
R
c
cellular resistance ( of apical and basolateral resistance) (·cm2)
-
R
p
resistance of the paracellular pathway (·cm2)
-
G
a
apical membrane conductance (mS·cm-2)
-
G
bl
basolateral membrane conductance (mS·cm-2)
-
G
p
paracellular conductance (mS·cm-2)
-
G
t
transepithelial conductance (mS·cm-2)
-
HT
contr
high transporting control epithelia
-
LT
contr
low transporting control epithelia
-
HT
aldo
aldosterone incubated high transporting epithelia
-
LT
aldo
aldosterone incubated low transporting epithelia 相似文献
48.
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Do muscle fiber properties commonly associated with fiber types in adult animals and the population distribution of these properties require normal activation patterns to develop? To address this issue, the activity of an oxidative [succinic dehydrogenase (SDH)] and a glycolytic [alpha-glycerophosphate dehydrogenase (GPD)] marker enzyme, the characteristics of myosin adenosinetriphosphatase (myosin ATPase, alkaline preincubation), and the cross-sectional area of single fibers were studied. The soleus and medial gastrocnemius of normal adult cats were compared with cats that 6 mo earlier had been spinally transected at T12-T13 at 2 wk of age. In control cats, SDH activity was higher in dark than light ATPase fibers in the soleus and higher in light than dark ATPase fibers in the medial gastrocnemius. After transection, SDH activity was similar to control in both muscles. GPD activity appeared to be elevated in some fibers in each fiber type in both muscles after transection. The cross-sectional areas most affected by spinal transection were light ATPase fibers of the soleus and dark ATPase fibers of the medial gastrocnemius, the predominant fiber type in each muscle. These data demonstrate that although the muscle fibers of cats spinalized at 2 wk of age presumably were never exposed to normal levels of activation, the activity of an oxidative marker enzyme was maintained or elevated 6 mo after spinal transection. Furthermore, although the absolute enzyme activities in some fibers were elevated by transection, three functional protein systems commonly associated with fiber types, i.e., hydrolysis of ATP by myosin ATPase and glycolytic (GPD) and oxidative (SHD) metabolism, developed in a coordinated manner typical of normal adult muscles. 相似文献
50.
Matthew Sorati Miriam Newman Ary A. Hoffmann 《Entomologia Experimentalis et Applicata》1996,78(3):283-290
Inbreeding effects and incompatibility relationships were examined in strains of the egg parasitoid Trichogramma nr brassicae (Hymenoptera: Trichogrammatidae) from southeastern Australia. Crosses between strains provided weak evidence of incompatibility in a few cases. However sex ratio in crosses within strains tended to be more female-biased than in crosses between strains. Inbreeding was imposed for four generations (F>0.59) of sib mating. The fitness of inbred strains was compared to that of outbred strains generated by crossing the inbred strains. No effects of inbreeding were found for any of the four female traits examined (fecundity, body length, head width and hind tibia length), indicating that T. nr. brassicae is not subjected to inbreeding depression. Inbreeding effects were also not found for male mating success as expected for the haploid sex. There were differences among strains for all traits apart from fecundity, indicating heritable variation. Strain differences for fitness measures were uncorrelated with wasp size. The potential use of inbreeding in the quality control of Trichogramma for mass-release is discussed. Inbreeding may be a useful tool in minimising the effects of laboratory adaptation, thereby extending the useful life of a strain. 相似文献