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排序方式: 共有154条查询结果,搜索用时 31 毫秒
31.
Markus Auer Clemens Gruber Marzia Bellei Katharina F. Pirker Marcel Zamocky Daniela Kroiss Stefan A. Teufer Stefan Hofbauer Monika Soudi Gianantonio Battistuzzi Paul G. Furtmüller Christian Obinger 《The Journal of biological chemistry》2013,288(38):27181-27199
Reconstructing the phylogenetic relationships of the main evolutionary lines of the mammalian
peroxidases lactoperoxidase and myeloperoxidase revealed the presence of novel bacterial heme
peroxidase subfamilies. Here, for the first time, an ancestral bacterial heme peroxidase is shown to
possess a very high bromide oxidation activity (besides conventional peroxidase activity). The
recombinant protein allowed monitoring of the autocatalytic peroxide-driven formation of covalent
heme to protein bonds. Thereby, the high spin ferric rhombic heme spectrum became similar to
lactoperoxidase, the standard reduction potential of the Fe(III)/Fe(II) couple shifted to more
positive values (−145 ± 10 mV at pH 7), and the conformational and thermal stability
of the protein increased significantly. We discuss structure-function relationships of this new
peroxidase in relation to its mammalian counterparts and ask for its putative physiological
role. 相似文献
32.
Kai K. Eberle K. E. Zinsmaier Sigrid Buchner M. Gruhn Mario Jenni Christine Arnold Christian Leibold Dietmar Reisch N. Walter Ernst Hafen A. Hofbauer Gert O. Pflugfelder E. Buchner 《Cell and tissue research》1998,294(2):203-217
The “cysteine string protein” (CSP) genes of higher eukaryotes code for a novel family of proteins characterized by a “J” domain and an unusual cysteine-rich region. Previous studies had localized the proteins in neuropil and synaptic terminals of larval and adult Drosophila and linked the temperature-sensitive paralysis of the mutants described here to conditional failure of synaptic transmission. We now use the null mutants as negative controls in order to reliably detect even low concentrations of CSPs by immunohistochemistry, employing three monoclonal antibodies. In wild-type flies high levels of cysteine string proteins are found not only in apparently all synaptic terminals of the embryonic, larval, and adult nervous systems, but also in the “tall cells” of the cardia, in the follicle cells of the ovary, in specific structures of the female spermatheca, and in the male testis and ejaculatory bulb. In addition, low levels of CSPs appear to be present in all tissues examined, including neuronal perikarya, axons, muscles, Malpighian tubules, and salivary glands. Western blots of isolated tissues demonstrate that of the four isoforms expressed in heads only the largest is found in non-neural organs. The wide expression of CSPs suggests that at least some of the various phenotypes of the null mutants observed at permissive temperatures, such as delayed development, short adult lifespan, modified electroretinogram, and optomotor behavior, may be caused by the lack of CSPs outside synaptic terminals. 相似文献
33.
Tilman D. Rachner Peggy Benad Martina Rauner Claudia Goettsch Shiv K. Singh Michael Schoppet Lorenz C. Hofbauer 《Journal of cellular biochemistry》2009,108(1):106-116
Osteoprotegerin (OPG) is a decoy receptor for receptor activator of NF‐κB ligand (RANKL) and TNF‐related apoptosis‐inducing ligand (TRAIL). While RANKL is essential for osteoclastogenesis and facilitates breast cancer migration into bone, TRAIL promotes breast cancer apoptosis. We analyzed the expression of OPG and TRAIL and its modulation in estrogen receptor‐positive MCF‐7 cells and receptor‐negative MDA‐MB‐231 cells. In both cells, OPG mRNA levels and protein secretion were dose‐ and time‐dependently enhanced by interleukin (IL)‐1β and suppressed by dexamethasone. In contrast to MCF‐7 cells, MDA‐MB‐231 abundantly expressed TRAIL mRNA, which was enhanced by IL‐1β and inhibited by dexamethasone. TRAIL activated pro‐apoptotic caspase‐3, ‐7, and poly‐ADP‐ribose polymerase and decreased cell numbers of MDA‐MB‐231, but had no effect on MCF‐7 cells. Gene silencing siRNA directed against OPG resulted in a 31% higher apoptotic rate compared to non‐target siRNA‐treated MDA‐MB‐231 cells. Furthermore, TRAIL induced significantly less apoptosis in cells cultured in conditioned media (containing OPG) compared to cells exposed to TRAIL in fresh medium lacking OPG (P < 0.01) and these protective effects were reversed by blocking OPG with its specific ligand RANKL (P < 0.05). The association between cancer cell survival and OPG production by MDA‐MB‐231 cells was further supported by the finding, that modulation of OPG secretion using IL‐1β or dexamethasone prior to TRAIL exposure resulted in decreased and increased rate of apoptosis, respectively (P < 0.05). Thus, OPG secretion by breast cancer cells is modulated by cytokines and dexamethasone, and may represent a critical resistance mechanism that protects against TRAIL‐induced apoptosis. J. Cell. Biochem. 108: 106–116, 2009. © 2009 Wiley‐Liss, Inc. 相似文献
34.
Tim van Zutphen Virginia Todde Rinse de Boer Martin Kreim Harald F. Hofbauer Heimo Wolinski Marten Veenhuis Ida J. van der Klei Sepp D. Kohlwein 《Molecular biology of the cell》2014,25(2):290-301
Cytosolic lipid droplets (LDs) are ubiquitous organelles in prokaryotes and eukaryotes that play a key role in cellular and organismal lipid homeostasis. Triacylglycerols (TAGs) and steryl esters, which are stored in LDs, are typically mobilized in growing cells or upon hormonal stimulation by LD-associated lipases and steryl ester hydrolases. Here we show that in the yeast Saccharomyces cerevisiae, LDs can also be turned over in vacuoles/lysosomes by a process that morphologically resembles microautophagy. A distinct set of proteins involved in LD autophagy is identified, which includes the core autophagic machinery but not Atg11 or Atg20. Thus LD autophagy is distinct from endoplasmic reticulum–autophagy, pexophagy, or mitophagy, despite the close association between these organelles. Atg15 is responsible for TAG breakdown in vacuoles and is required to support growth when de novo fatty acid synthesis is compromised. Furthermore, none of the core autophagy proteins, including Atg1 and Atg8, is required for LD formation in yeast. 相似文献
35.
Josef Hofbauer 《Journal of mathematical biology》1996,34(5-6):675-688
We review some properties of the evolutionary dynamics for asymmetric conflicts, give a simplified approach to them, and present
some new results on the stability and bifurcations occurring in these conservative systems. In particular, we compare their
dynamics to those of Hamiltonian systems 相似文献
36.
The dynamic stability of an evolutionarily stable strategy (ESS) is analyzed for a diploid species under individual viability selection. An individual's viability depends on the genotypic frequencies at a single autosomal locus through a payoff matrix determined by phenotypic behaviours (i.e. strategies). It is shown that an ESS of this payoff matrix is dynamically stable if there are at most three alleles — an intuitive result that strengthens the importance of static game-theoretic methods in genetic models.Author for correspondence 相似文献
37.
Summary A procedure for the electron microscopic autoradiography of Al adenosine receptors is described. Fresh tissue slices from rat hippocampus were incubated with the radioactive adenosine analogs: Cyclohexyl[3H]adenosine, 5-N-ethylcarboxamido[3H]adenosine or [125I]-iodohydroxyphenylisopropyladenosine. Various fixation agents were tested with respect to the retention of these ligands by the tissue. While most of the ligands were lost in aldehyde fixation they were retained by osmium tetroxide probably via a crosslinking reaction. The final method of choice was an aldehyde prefixation (in the case of [125I]-iodohydroxyphenylisopropyladenosine with 4% buffered paraformaldehyde) during which more than 90% of the nonspecifically bound ligands were washed out while 40% of the specifically bound ligands remained. Subsequent fixation with osmium tetroxide (1%) allowed a standard protocoll for dehydration and embedding to be used with only minimal (less than 5%) further loss of the ligands. Electron microscopic autoradiography provided evidence for a specific distribution of the binding sites for [125I]-iodohydroxyphenylisopropyladenosine.Abbreviations GA
Glutardialdehyde
- PFA
Paraformaldehyde
- OsO4
Osmiumtetroxide
- CHA
Cyclohexyladenosine
- NECA
N-ethyl-carboxamidoadenosine
- PIA
Phenylisopropyladenosine
- I-HPIA
Iodohydroxyphenylisopropyladenosine
- HPIA
Hydroxyphenylisopropyladenosine 相似文献
38.
Summary The larval and early pupal development of the optic lobes in Drosophila is described qualitatively and quantitatively using [3H]thymidine autoradiography on 2-m plastic sections. The optic lobes develop from 30–40 precursor cells present in each hemisphere of the freshly hatched larva. During the first and second larval instars, these cells develop to neuroblasts arranged in two epithelial optic anlagen. In the third larval instar and in the early pupa these neuroblasts generate the cells of the imaginal optic lobes at discrete proliferation zones, which can be correlated with individual visual neuropils.The different neuropils as well as the repetitive elements of each neuropil are generated in a defined temporal sequence. Cells of the medulla are the first to become postmitotic with the onset of the third larval instar, followed by cells of the lobula complex and finally of the lamina at about the middle of the third instar. The elements of each neuropil connected to the most posterior part of the retina are generated first, elements corresponding to the most anterior retina are generated last.The proliferation pattern of neuroblasts into ganglion mother cells and ganglion cells is likely to include equal as well as unequal divisions of neuroblasts, followed by one or two generations of ganglion mother cells. For the lamina the proliferation pattern and its temporal coordination with the differentiation of the retina are shown. 相似文献
39.
Chondrocytes in situ experience fluctuations in extracellular osmolarity resulting from mechanical loading. The objective of this study was to determine whether hyperosmotic stress causes or exacerbates interleukin-1 (IL-1)-mediated effects in bovine articular cartilage. Disks of cartilage cut from the articular surface of calf radiocarpal joints were incubated for 24h in the presence or absence of IL-1 in Dulbecco's modified Eagle's medium adjusted to various osmolalities with sucrose or NaCl. Cyclooxygenase (COX)-2 levels in the cartilage were examined by Western blot. Culture media were assayed for prostaglandin E(2) (PGE(2)), nitrite as an indicator of nitric oxide (NO) production, and sulfated glycosaminoglycan as an indicator of proteoglycan degradation. We report the osmolality-dependent potentiation of COX-2 and PGE(2) production, and the osmolality-dependent inhibition of NO production and proteoglycan degradation in IL-1-activated cartilage. The data demonstrate that osmotic and cytokine signaling interact to differentially modulate IL-1-stimulated effects in calf articular cartilage. 相似文献