Anceaux's Glasses

The Kayapó (Disappearing World Series) Producer: Michael Beckham; Director: Michael Beckham; Camera: Michael Blakeley; Sound: David Woods; Editor: Paul Griffiths‐Davies; Consultant Anthropologist: Terence Turner; Distributor: Enquiries to Granada Television International, 1221 6th Avenue, Suite 3468, New York, NY 10020 USA, (212) 809–8480. 1987, 52 min., color. Original: 16mm, only available for distribution on video. Language: Original Kayapo, Narration English, Subtitles English.

Independent Films and Videos from Latin America: Producers’ Obstacles, National Diversity, and the Cultural Woes of Their Foreign Audiences Review of Democracy in Communication: Popular Film and Video in Latin America. A catalogue compilation of various videotapes. 1988, color. Original language: Spanish, English subtitles. Distributed by: Karen Ranucci, 124 Washington Place, New York, NY, tele. 212–463–9198.     

A waveguide imaging platform for live‐cell TIRF imaging of neurons over large fields of view

Large fields of view (FOVs) in total internal reflection fluorescence microscopy (TIRFM) via waveguides have been shown to be highly beneficial for single molecule localisation microscopy on fixed cells [1,2] and have also been demonstrated for short‐term live‐imaging of robust cell types [3‐5], but not yet for delicate primary neurons nor over extended periods of time. Here, we present a waveguide‐based TIRFM set‐up for live‐cell imaging of demanding samples. Using the developed microscope, referred to as the ChipScope, we demonstrate successful culturing and imaging of fibroblasts, primary rat hippocampal neurons and axons of Xenopus retinal ganglion cells (RGCs). The high contrast and gentle illumination mode provided by TIRFM coupled with the exceptionally large excitation areas and superior illumination homogeneity offered by photonic waveguides have potential for a wide application span in neuroscience applications.