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61.
P. Ayazi Shamlou S. Stavrinides N. Titchener-Hooker M. Hoare 《Bioprocess and biosystems engineering》1996,14(5):237-243
Experimental data relating to the breakage of isoelectric Soya protein precipitates in a mechanically agitated bioreactor are provided and examined in the light of a proposed mechanistic model which relates the size of the maximum attainable aggregate diameter to the energy dissipation rate in the vessel. The analysis suggests that protein precipitation results in the formation of scale-invariant fractal aggregates with a dimensionality of 2.2. Comparing the fractal dimensionality of the protein precipitates with reported values based on computer simulation studies suggests that the aggregates undergo considerable restructuring during agitation.List of Symbols
A
Hamaker constant (J)
-
D
impeller diameter (m)
-
d
p
primary particle diameter (m)
-
d
f
maximum aggregate diameter (m)
-
G
shear rate (s–1)
-
H
0
separation distance between two primary particles (m)
-
k
constant in Eq. (5)
-
K
constant in Eq. (6)
-
N
impeller speed (rpm or rps)
-
r
radial position in an aggregate, measured from the centre (m)
-
t
time of exposure to shear (mins)
-
T
e
eddy period (s–1)
-
v
f
aggregate volume (m3)
Greek Symbols
aggregate dimensionality constant
-
energy dissipation rate (W/kg)
-
dynamic viscosity of particle-free liquid (kg/ms)
-
kinematic viscosity of particle-free liquid (m2/s)
-
collision probability (–)
-
p
aggregate density (kg/m3)
-
p
continuous phase density (kg/m3)
-
aggregate mechanical strength (N/m2)
-
shear stress (N/m2)
-
particle concentration in an aggregate (m3/m3)
-
(r)
porosity at radial position, r 相似文献
62.
The effect of temperature on non-specific defence parameters of three strains of juvenile Atlantic halibut (Hippoglossus hippoglossus L.) 总被引:2,自引:0,他引:2
Langston AL Hoare R Stefansson M Fitzgerald R Wergeland H Mulcahy M 《Fish & shellfish immunology》2002,12(1):61-76
The effect of temperature (8, 12, 15 and 18 degrees C) on a variety of non-specific defence and haematological parameters was examined in three geographically distinct reared strains (Canadian, Icelandic, Norwegian) of Atlantic halibut. The results indicate that temperature exerts a considerable influence on some blood parameters (packed cell volume and the percentage population of leucocytes in peripheral blood) and on some humoral parameters (serum lysozyme activity and serum protein levels) of halibut. A high temperature of 18 degrees C caused a decrease in the number of circulating blood cells and an increase in serum lysozyme levels; effects consistent with those reported within the literature for stress. The different strains of halibut exhibited differing responses with respect to differential counts of peripheral blood lymphocytes and thrombocytes, and to serum protein concentrations, serum lysozyme activity, serum iron content, unsaturated iron binding capacity of serum and O2- production by kidney macrophages. 相似文献
63.
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D. J. Hoare A. J. W. Ward I. D. Couzin D. P. Croft J. Krause 《Journal of fish biology》2001,59(6):1667-1672
The design and operation of a grid-net are described, which allows the capture of entire shoals of wild fish and preserves information about the two-dimensional structure of shoals and the spatial positions of individual fish within the shoal. This simple technique facilitates investigation of several aspects of individual differences in fish shoaling behaviour in the wild for the first time. 相似文献
65.
Acetate assimilation by Nitrobacter agilis in relation to its "obligate autotrophy" 总被引:7,自引:14,他引:7 下载免费PDF全文
Acetate (1 to 10 mm) had no effect on the rate of nitrite oxidation or exponential growth by Nitrobacter agilis. However, acetate-1-(14)C and -2-(14)C were both assimilated by growing cultures, and acetate carbon contributed 33 to 39% of newly synthesized cell carbon. Carbon from acetate was incorporated into all of the major cell constituents, including most of the amino acids of cell protein and poly-beta-hydroxybutyrate (PHB). Cultures grown in the presence of acetate showed a significant increase in turbidity, attributable in part to protein synthesis and the accumulation of PHB in the "post-exponential phase," when the supply of nitrite was completely exhausted. Cell suspensons of N. agilis assimilated acetate in the absence of bicarbonate and even in the absence of nitrite. However, the addition of nitrite increased the rate of acetate assimilation by cell suspensions. The distribution of (14)C-acetate incorporated by cell suspensions was qualitatively similar to that found with growing cultures. Cell suspensions of N. agilis slowly oxidized acetate to CO(2). Addition of nitrite suppressed CO(2) production from acetate but increased the assimilation of acetate carbon into cell material. N. agilis contained all the enzymes of the tricarboxylic acid cycle. Growth of N. agilis in the presence of acetate did not significantly affect the levels of the enzymes of the tricarboxylic acid cycle, but did result in a 100-fold increase in the specific activity of isocitratase. In contrast, carboxydismutase was partially repressed. N. agilis was grown heterotrophically through seven transfers on a medium containing acetate and casein hydrolysate. The addition of nitrite increased the rate of heterotrophic growth. Heterotrophically grown organisms still retained their ability to grow autotrophically with nitrite. However, these organisms oxidized nitrite at a slower rate. Organisms from autotrophic and heterotrophic cultures were analyzed to determine the mean guanine plus cytosine content of their deoxyribonucleic acid; in both cases this mean was 61.2 +/- 1%. We concluded that N. agilis is not an obligate autotroph; it appears to be a facultative autotroph which resembles the novel facultative autotroph, Thiobacillus intermedius, very closely. 相似文献
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70.
Ravula SB Yu J Tran JA Arellano M Tucci FC Moree WJ Li BF Petroski RE Wen J Malany S Hoare SR Madan A Crowe PD Beaton G 《Bioorganic & medicinal chemistry letters》2012,22(1):421-426
The structure-activity relationships of 2-(piperidin-3-yl)-1H-benzimidazoles, 2-morpholine and 2-thiomorpholin-2-yl-1H-benzimidazoles are described. In the lead optimization process, the pK(a) and/or logP of benzimidazole analogs were reduced either by attachment of polar substituents to the piperidine nitrogen or incorporation of heteroatoms into the piperidine heterocycle. Compounds 9a and 9b in the morpholine series and 10g in the thiomorpholine series demonstrated improved selectivity and CNS profiles compared to lead compound 2 and these are potential candidates for evaluation as sedative hypnotics. 相似文献